Results 141 to 150 of about 90,091 (319)

Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]

, 2020
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F., Hunka, Julia, Riley, John T   +2 more
core   +1 more source

Analysis and Functional Consequences of Increased Fab-Sialylation of Intravenous Immunoglobulin (IVIG) after Lectin Fractionation [PDF]

, 2012
It has been proposed that the anti-inflammatory effects of intravenous immunoglobulin (IVIG) might be due to the small fraction of Fc-sialylated IgG.
A Baerenwaldt, A Ephrem, Adrian W. Zuercher, Andreas Hofmann, AS De Groot, AW Zuercher, B Tackenberg, C Ramakrishna, D Leontyev, David J. Boerema, F Nimmerjahn, Fabian Käsermann, HP Hartung, HP Hartung, I Schwab, J Bayry, J Stadlmann, JF Seite, L Padet, LM Araujo, M Ballow, M Basta, M Debre, MD Kazatchkine, Monika Rüegsegger, MS Maddur, NG Karlsson, P Imbach, P Imbach, RM Anthony, RM Anthony, RM Anthony, Robert B. Sim, S Hara, Sandra Wymann, SC Jordan, SV Kaveri, SV Kaveri, Sylvia Miescher, T Guhr, T Tha-In, TV Arumugam, Y Arnson, Y Kaneko   +43 more
core   +2 more sources

Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds

Frontiers in Immunology, 2017
Therapeutic monoclonal antibodies have become molecules of choice to treat autoimmune disorders, inflammatory diseases, and cancer. Moreover, bispecific/multispecific antibodies that target more than one antigen or epitope on a target cell or recruit ...
Hongyan Liu, A. Saxena, S. Sidhu, Donghui Wu   +3 more
semanticscholar   +1 more source

Eosinophils‐Induced Lumican Secretion by Synovial Fibroblasts Alleviates Cartilage Degradation via the TGF‐β Pathway Mediated by Anxa1 Binding

Advanced Science, EarlyView.
Eosinophils play a crucial role in the progression of temporomandibular joint osteoarthritis (TMJOA). This study demonstrates that eosinophils, induced by OVA/IL‐5, promote Lumican secretion in the synovium, which binds to Annexin A1 in chondrocytes, inhibiting transforming growth factor β2 and Smad2/3 phosphorylation.
Wenqian Chen, Yuwei Zhou, Wenxiu Yuan, Yanjing Ou, Hanyu Lin, Kaixun He, Xueshen Qian, Huachen Chen, Chengchaozi Wang, Jie Lu, Weiping Chen, Dexiong Li, Jiang Chen   +12 more
wiley   +1 more source

Intermediate DNA at low added salt: DNA bubbles slow the diffusion of short DNA fragments [PDF]

arXiv, 2010
We report a study of DNA (150 bp fragments) conformations in very low added salt $<0.05$mM, across wide DNA concentration range $0.0015\leq c \leq 8$~mM (bp). We found an intermediate DNA conformation in the region $0.05 < c < 1$~mM, by means of fluorescence correlation spectroscopy (FCS) and UV-absorbance measurements. FCS detected that in this region
arxiv  

Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: implications for B-cell selection

, 2018
Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings have proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic.
Amara, Khaled, Baeten, Dominique, Gabay, Cem, Gronwall, Caroline, Israelsson, Lena, Klareskog, Lars, Lanzavecchia, Antonio, Lloyd, Katy A., Lundberg, Karin, Lundstrom, Susanna L., Malmstrom, Vivianne, Mueller, Daniel L., Piccoli, Luca, Reed, Evan, Steen, Johanna, Titcombe, Philip J., Zhou, Diana, Zubarev, Roman A.   +17 more
core   +1 more source

A Solution‐Based Deposition Method Enabling Pigment Blue Edible Electrochemical Transistors

Advanced Science, EarlyView.
A novel solution‐based method is presented to produce Copper Phthalocyanine fibrous films displaying effective ion permeability and enhanced transconductance in electrolyte‐gated transistors architectures. Using these films, the first instance of a fully edible electrolyte‐gated transistor with volumetric capacitance, targeting in‐body applications, is
Alessandro Luzio, Fabrizio M. Ferrarese, Matteo Butti, Alberto D. Scaccabarozzi, Bojan Petrović, Sanja Kojić, Goran Stojanović, Simone Fiorini Granieri, Shubham Tanwar, Adrica Kyndiah, Mario Caironi   +10 more
wiley   +1 more source

Detection and Characterization of Paraproteinemia in Canine Chronic B‐cell Lymphocytic Leukemia Using Routine and Free Light Chain Immunofixation

Veterinary Clinical Pathology, Volume 51, Issue 4, Page 551-559, December 2022., 2022
Abstract Background Hyperglobulinemia is reported in 26% of canine chronic B‐cell lymphocytic leukemia (B‐CLL) cases. However, few cases have been characterized by protein electrophoresis and immunofixation (IF), and the incidence of a monoclonal protein (M‐protein) is unknown using these techniques.
R. Adam Harris, Erik B. Gary, Emily D. Rout, Anne C. Avery, A. Russell Moore   +4 more
wiley   +1 more source

Generation of Site‐Specifically Labeled Affinity Reagents via Use of a Self‐Labeling Single Domain Antibody

Advanced Science, EarlyView.
Several methods have been used to label antibodies to facilitate visualization of cognate targets. Here, we leverage a new variety of conjugation chemistry, based on a crosslinking nanobody, to label target‐specific nanobody constructs with functionalities of choice.
Stanley Fayn, Swarnali Roy, Chino C. Cabalteja, Woonghee Lee, Hima Makala, Kwamena Baidoo, Divya Nambiar, Julia Sheehan‐Klenk, Joon‐Yong Chung, Jesse Buffington, Mitchell Ho, Freddy E. Escorcia, Ross W. Cheloha   +12 more
wiley   +1 more source

A Low-temperature 1H NMR Study of H2O and D2O Associated Competitively with Immunoglobulin G in Solution [PDF]

arXiv, 2007
An approach has been proposed to characterize the competitive association of D2O and to study the heterogeneity of hydration water adsorbed by the protein, immunoglobulin G, using methodology for determining of non-freezing water in mixed H2O-D2O protein solutions by low-temperature 1H NMR technique. Direct data on the numbers of deuteriums adsorbed by
arxiv