Results 251 to 260 of about 158,982 (309)

A comparison of strategies to stabilize immunoglobulin Fv-fragments

Biochemistry, 1990
Fv-Fragments of antibodies may dissociate at low protein concentrations and are too unstable for many applications at physiological temperatures. To stabilize Fv-fragments against dissociation, we have tested and compared three different strategies on the Fv-fragment of the well-characterized phosphocholine binding antibody McPC603 expressed and ...
R, Glockshuber, M, Malia, I, Pfitzinger, A, Plückthun   +3 more
openaire   +4 more sources

Fragmentation of Immunoglobulin G

Current Protocols in Immunology, 1997
AbstractThis unit describes procedures for fragmentation of IgG to the monovalent Fab fragment using papain digestion, and to the bivalent F(ab′)2 fragment by pepsin digestion. Alternative methods of fragmentation to F(ab′)2 include use of papain that is preactivated with cysteine and use of the enzyme ficin.
Sarah M, Andrew, Julie A, Titus
openaire   +4 more sources

FRAGMENTATION OF IMMUNOGLOBULIN DURING STORAGE

Canadian Journal of Biochemistry, 1966
Solutions of human γ-globulin undergo changes on storage resulting in the formation of fragments similar to those produced by the digestion of human γ-globulin with the enzymes papain and plasmin. Two fragments have been isolated from the naturally fragmented material which resemble the Fab and Fc fragments resulting from digestion by papain.
G E, Connell, R H, Painter
openaire   +2 more sources

Immunoglobulin genes in DNA restriction fragments

Biochemistry, 1978
We have investigated the organization of immunoglobulin genes in mice. High molecular weight DNA from myelomas and Krebs ascites cells was cleaved with EcoRI restriction endonuclease and fractionated using preparative agarose gel electrophoresis. Each fraction was then hybridized to an immunoglobulin mRNA or a cDNA transcribed from the mRNA.
R, Wilson, A M, Walfield, A, Murakami, U, Storb   +3 more
openaire   +2 more sources

Fragmentation of Therapeutic Human Immunoglobulin Preparations

Vox Sanguinis, 1995
AbstractSome commercial batches of human therapeutic immunoglobulins (Ig) have been found to show evidence of molecular fragmentation when examined by molecular sizing methodologies including sodium dodecyl sulphate polyacrylamide gel electrophoresis [SDS‐PAGE] and size exclusion high performance liquid chromatography (SE‐HPLC).
M, Page, C, Ling, P, Dilger, M, Bentley, T, Forsey, C, Longstaff, R, Thorpe   +6 more
openaire   +2 more sources

FRAGMENTS OF IMMUNOGLOBULINS IN HUMAN FAECES

Acta Pathologica Microbiologica Scandinavica Section C Immunology, 1976
Fab‐fragments of IgG were easily demonstrated in extracts of faeces from healthy infants and children. Employing immunoelectrophoresis with antisera to whole human serum and to Fab‐fragments of IgG, a marked precipitation line, likely to represent such fragments, was evident in the cathodal or γ‐region.
B, Haneberg, C, Endresen
openaire   +2 more sources

Characterization of immunoglobulin G fragments in liquid intravenous immunoglobulin products

Transfusion, 2005
BACKGROUND: Intravenous immunoglobulin (IVIG) products formulated as a liquid instead of a powder have become commercially available. Preferably, such liquid products should not alter after storage outside the refrigerator. Therefore, a thorough characterization of immunoglobulin G (IgG) fragmentation at various storage temperatures is required.STUDY ...
Robert V, Diemel, Hendricus G J, ter Hart, Gerardus J A, Derksen, Anky H L, Koenderman, Rob C, Aalberse   +4 more
openaire   +2 more sources

Aggregation of immunoglobulin fragments in concentrated urine

Clinica Chimica Acta, 1967
Abstract Normal human urine was concentrated by ultrafiltration and chromatographed on a Sephadex G-100 gel column. Fractions containing immunoglobulin subunits, either intact L and H polypeptide chains or fragments thereof were isolated, labelled with 131 I, diluted in the ultrafiltrate and subsequently reconcentrated by ultrafiltration. A portion
J R, Poortmans, K J, Bloch, R W, Jeanloz
openaire   +2 more sources

Bacterial expression of immunoglobulin fragments

Current Opinion in Immunology, 1993
The expression of Ig fragments in Escherichia coli permits rapid access to engineered molecules with antigen-binding properties. While the expression in a functional state by secretion to the periplasm is the standard method for the production of Fv and Fab fragments, single chain Fv fragments are mainly produced by refolding from insoluble aggregates.
openaire   +3 more sources

Immunoglobulin Fab fragment-binding proteins

International Journal of Immunopharmacology, 1994
Five molecules are known to bind the Fab fragments of human immunoglobulins (Ig). Microbial protein A and protein G are primarily Fc-binding molecules but can also bind other structures of the heavy chain, which are located in the variable domain of the third subgroup (VH3) and in the first constant domain of IgG (CH1 gamma), respectively. In contrast,
openaire   +2 more sources