Advanced Science, EarlyView.Novel ATR/PARP1 dual inhibitors are developed for the first time. Advanced lead B8 effectively reduces cell viability in vitro and suppresses tumor growth in vivo, with better potency than ATRi and PARPi alone or in combination. Mechanistically, B8 induces apoptosis, arrests the cell cycle, and inhibits cancer cells colony formation, migration, and ...
Yuan Gao +13 more
wiley +1 more source
Mass Spectrometry Reviews, EarlyView., 2023 Abstract Analysis of triacylglycerol (TG) and phospholipid sn‐positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods.
Mikael Fabritius, Baoru Yang
wiley +1 more source
Genetic analysis of self-associating immunoglobulin G rheumatoid factors from two rheumatoid synovia implicates an antigen-driven response. [PDF]
, 1992 Although much has been learned about the molecular basis of immunoglobulin M (IgM) rheumatoid factors (RFs) in healthy individuals and in patients with mixed cryoglobulinemia and rheumatoid arthritis, little is known about the genetic origins of the ...
Carson, DA +7 more
core
De novo inference of diversity genes and analysis of non-canonical V(DD)J recombination in immunoglobulins [PDF]
arXiv, 2019 The V(D)J recombination forms the immunoglobulin genes by joining the variable (V), diversity (D), and joining (J) germline genes. Since variations in germline genes have been linked to various diseases, personalized immunogenomics aims at finding alleles of germline genes across various patients.
arxiv
Potent Cross‐neutralizing Antibodies Reveal Vulnerabilities of Henipavirus Fusion Glycoprotein
Advanced Science, EarlyView.Hendra and Nipah viruses (HNVs) pose significant threats to global health. This work reports potent cross‐neutralizing antibodies targeting the fusion glycoprotein (F) and reveals shared features of these antibodies and vulnerabilities of F, thereby guiding the rational design of countermeasures against HNVs and similar pathogens.
Yi Ren +19 more
wiley +1 more source
The partial sequence of two large peptides from the N-terminal half of heavy chains from normal rabbit immunoglobulin G [PDF]
, 1968 John J. Cebra +2 more
openalex +1 more source
Advanced Science, EarlyView.Abnormal mechanical stretch induced by mechanical ventilation (MV) disrupts endothelial junctions via the NOX2/ROS/CaMKII/ERK1/2 axis both in human aortic endothelial cells (HAECs) in vitro and in ventilator‐induced lung injury (VILI) in mice. Quercetin, an antioxidant predicted to bind to NOX2, can effectively prevent mechanical stretch‐induced ...
Tao Jiang +8 more
wiley +1 more source
In-Cell Assembly of scFv from Human Thyroid- Infiltrating B Cells
BioTechniques, 1997 The construction of a large library of single-chain Fv (scFv) antibody fragments involves a random assortment of heavy and light chains. Although useful for the production of recombinant antibodies, this method is not adapted to the study of the ...
N. Chapal +6 more
doaj +1 more source
EVIDENCE FOR MULTIPLE GENE CONTROL OF A SINGLE POLYPEPTIDE CHAIN: THE HEAVY CHAIN OF RABBIT IMMUNOGLOBULIN [PDF]
, 1969 Marian Elliott Koshland +2 more
openalex +1 more source
Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli.
Nucleic Acids Research, 1984 Genes for a murine mu heavy chain and a lambda light chain immunoglobulin have been inserted into bacterial expression plasmids containing the Escherichia coli trp promoter and ribosome binding site.
M. Boss +3 more
semanticscholar +1 more source