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β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation) [PDF]
Bio-Protocol, 2013 Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein.
Ralph Boettcher
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A comparison of four technologies for detecting p53 aggregates in ovarian cancer
Frontiers in Oncology, 2022 The tumor suppressor protein p53 is mutated in half of all cancers and has been described to form amyloid-like structures, commonly known from key proteins in neurodegenerative diseases.
Nicole Heinzl +9 more
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Cells, 2023 The metabotropic glutamate receptor 1 (mGlu1) plays a pivotal role in synaptic transmission and neuronal plasticity. Despite the fact that several interacting proteins involved in the mGlu1 subcellular trafficking and intracellular transduction ...
Mahnaz Mansouri +10 more
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Cells, 2022 The detection, manipulation and purification of proteins is key in modern life sciences studies. To achieve this goal, a plethora of epitope tags have been employed in model organisms from bacteria to humans.
Catia Igreja +7 more
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Cold Spring Harbor Protocols, 2017 This immunoprecipitation protocol details individual steps for the enrichment and purification process of specific proteins from a complex cell lysate using an antibody bound to a solid matrix. Purified antigen(s) can be eluted by various methods, and the resultant protein target can be analyzed and/or identified by numerous assays, including the ...
James, DeCaprio, Thomas O, Kohl
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Frontiers in Immunology, 2022 BackgroundAcinetobacter baumannii is one of the most life-threatening multidrug-resistant pathogens worldwide. Currently, 50%–70% of clinical isolates of A. baumannii are extensively drug-resistant, and available antibiotic options against A.
Mustafa Burak Acar +10 more
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Chromatin Immunoprecipitation [PDF]
Cold Spring Harbor Protocols, 2020 Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in vivo, followed by the extraction of chromatin from cross-linked cells and tissues.
James, DeCaprio, Thomas O, Kohl
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Biomolecules, 2021 Epitope tags are widely employed as tools to detect, purify and manipulate proteins in various experimental systems. We recently introduced the ALFA-tag together with two ALFA-specific single-domain antibodies (sdAbs), NbALFA and NbALFAPE, featuring high
Markus Kilisch +5 more
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Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex [PDF]
, 2012 BACKGROUND: Mutations in the chromodomain helicase DNA binding protein 7 gene (CHD7) lead to CHARGE syndrome, an autosomal dominant multiple malformation disorder. Proteins involved in chromatin remodeling typically act in multiprotein complexes.
Batsukh, Tserendulam +7 more
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Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads
Metabolites, 2022 Blood samples are minimally invasive and can be collected repeatedly, but they are far from the site of disease and the target molecules are diluted by the large amount of blood. Therefore, we performed lipidomics using immunoprecipitation as a method to
Suzumi M. Tokuoka +5 more
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