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Selection and optimization strategy for Rap1-targeting single-domain antibodies as platelet activation markers. [PDF]
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Current Protocols in Neuroscience, 1999
AbstractImmunoprecipitation is a technique in which an antigen is isolated by binding to a specific antibody attached to a sedimentable matrix. It is also used to analyze protein fractions separated by other biochemical techniques such as gel filtration or density gradient sedimentation.
J S, Bonifacino +2 more
openaire +7 more sources
AbstractImmunoprecipitation is a technique in which an antigen is isolated by binding to a specific antibody attached to a sedimentable matrix. It is also used to analyze protein fractions separated by other biochemical techniques such as gel filtration or density gradient sedimentation.
J S, Bonifacino +2 more
openaire +7 more sources
Current Protocols in Cell Biology, 1998
AbstractSelective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein‐protein interactions. Clear step‐by‐step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation.
J S, Bonifacino, E C, Dell'Angelica
openaire +2 more sources
AbstractSelective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein‐protein interactions. Clear step‐by‐step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation.
J S, Bonifacino, E C, Dell'Angelica
openaire +2 more sources
2013
Recent studies have elucidated molecular mechanisms underlying the transcriptional control of metabolism in complex metabolic disorders such as metabolic syndrome and atherosclerosis. Chromatin immunoprecipitation (ChIP) is an important technique to study protein-DNA interactions in vivo.
Grant D, Barish, Rajenda K, Tangirala
+6 more sources
Recent studies have elucidated molecular mechanisms underlying the transcriptional control of metabolism in complex metabolic disorders such as metabolic syndrome and atherosclerosis. Chromatin immunoprecipitation (ChIP) is an important technique to study protein-DNA interactions in vivo.
Grant D, Barish, Rajenda K, Tangirala
+6 more sources
Current Protocols in Cell Biology, 2016
AbstractSelective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein‐protein interactions. Clear step‐by‐step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation.
Juan S, Bonifacino +2 more
openaire +2 more sources
AbstractSelective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein‐protein interactions. Clear step‐by‐step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation.
Juan S, Bonifacino +2 more
openaire +2 more sources
2003
Immunoprecipitation allows the investigator to detect and quantitate antigens in a mixture of proteins or characterize a specific antibody response to already well-characterized proteins. Addition of antibodies to proteins, usually radiolabeled, allows formation of antigen-antibody complexes.
K, Johansen, L, Svensson
openaire +2 more sources
Immunoprecipitation allows the investigator to detect and quantitate antigens in a mixture of proteins or characterize a specific antibody response to already well-characterized proteins. Addition of antibodies to proteins, usually radiolabeled, allows formation of antigen-antibody complexes.
K, Johansen, L, Svensson
openaire +2 more sources
2018
Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome.
Julio C, Ayala +2 more
openaire +2 more sources
Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome.
Julio C, Ayala +2 more
openaire +2 more sources