Results 261 to 270 of about 487,581 (283)
Some of the next articles are maybe not open access.
2016
Chromatin immunoprecipitation (ChIP) is a valuable method to investigate protein-DNA interactions in vivo. Since its discovery it has been indispensable to identify binding sites and patterns of a variety of DNA-interacting proteins, such as transcription factors and regulators, modified histones, and epigenetic modifiers.
Laura, Wiehle, Achim, Breiling
openaire +2 more sources
Chromatin immunoprecipitation (ChIP) is a valuable method to investigate protein-DNA interactions in vivo. Since its discovery it has been indispensable to identify binding sites and patterns of a variety of DNA-interacting proteins, such as transcription factors and regulators, modified histones, and epigenetic modifiers.
Laura, Wiehle, Achim, Breiling
openaire +2 more sources
2022
Co-immunoprecipitation is a well-established technique for determining whether two proteins interact. It is based on the principle that by pulling down one protein, you will also obtain any other proteins that exist in a complex with that protein. It is a relatively simple technique that does not require expensive reagents or materials.
Ian M, Evans, Ketevan, Paliashvili
openaire +2 more sources
Co-immunoprecipitation is a well-established technique for determining whether two proteins interact. It is based on the principle that by pulling down one protein, you will also obtain any other proteins that exist in a complex with that protein. It is a relatively simple technique that does not require expensive reagents or materials.
Ian M, Evans, Ketevan, Paliashvili
openaire +2 more sources
Chromatin Immunoprecipitation (ChIP)
Cold Spring Harbor Protocols, 2009INTRODUCTIONChromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with
Michael F, Carey +2 more
openaire +2 more sources
Chromatin Immunoprecipitation Assays
2004Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain.
Yan, Yan, Haobin, Chen, Max, Costa
openaire +2 more sources
Immunoprecipitation: Denaturing Lysis
Cold Spring Harbor Protocols, 2006INTRODUCTIONFor many sources of antigens, one useful method of lysis is to treat cells with harsh, denaturing solutions to release most of the protein antigens, as described here. The lysates are then diluted to reduce the denaturing conditions to levels that are suitable for the formation of antibody-antigen complexes.
Ed, Harlow, David, Lane
openaire +2 more sources
Immunoprecipitation of chromatin
1996Publisher Summary This chapter discusses the immunoprecipitation of chromatin. The chapter discusses three standard protocols, all of which are used to analyze the role of specific proteins within defined regions of the eukaryotic genome. It analyzes both the DNA and the protein after immunoprecipitation.
L P, O'Neill, B M, Turner
openaire +2 more sources
Making Protein Immunoprecipitates
2004A wide variety of methods used in the study of signal transduction in eukaryotes rely on the ability to precipitate proteins from whole cell extracts. Immunoprecipitation and related methods of affinity purification are routinely used to assess binding partner interactions and enzyme activity in addition to the size of a protein, rates of protein ...
Elaine A, Elion, Yunmei, Wang
openaire +2 more sources
Plant Chromatin Immunoprecipitation
2010Development of multicellular organisms is based on specialized gene expression programs. Because chromatin establishes the environment for transcription, understanding composition and dynamics of chromatin is an important part of developmental biology.
Villar, C., Köhler, C.
openaire +3 more sources
Native Chromatin Immunoprecipitation
2004Chromatin immunoprecipitation (ChIP) is a technique widely used for determining the genomic location of modified histones and other chromatin-associated factors. Here we describe the methodology we have used in our laboratory for the immunoprecipitation of chromatin isolated from cells in the absence of crosslinking.
Thorne, Alan, Myers, Fiona, Hebbes, Tim
openaire +2 more sources
2016
The relevance of RNA-protein interactions in modulating mRNA and noncoding RNA function is increasingly appreciated and several methods have been recently developed to map them. The RNA immunoprecipitation (RIP) is a powerful method to study the physical association between individual proteins and RNA molecules in vivo.
Gagliardi Miriam, Matarazzo Maria R
openaire +3 more sources
The relevance of RNA-protein interactions in modulating mRNA and noncoding RNA function is increasingly appreciated and several methods have been recently developed to map them. The RNA immunoprecipitation (RIP) is a powerful method to study the physical association between individual proteins and RNA molecules in vivo.
Gagliardi Miriam, Matarazzo Maria R
openaire +3 more sources