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Immunoprecipitation and SDS-PAGE for Cross-Linking Immunoprecipitation (CLIP)
Cold Spring Harbor Protocols, 2018This first part of this protocol is designed to optimize purification of the RNABP by immunoprecipitation for cross-linking immunoprecipitation (CLIP) experiments. The key variables to assess are the quality and quantity of antibody needed to immunoprecipitate most but not quite all of the RNABP (the titration will decrease nonspecific binding), and ...
Jennifer C, Darnell +3 more
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Methyl DNA Immunoprecipitation
2009Epigenetics is the study of heritable changes in gene expression. Chromatin immunoprecipitation (ChIP) and methylation status analysis of genes have been applied to the study of epigenetic modifications, often perturbed in human cancer. ChIP is a technique allowing the analysis of the protein association with specific genomic regions in the context of ...
Juana, Magdalena, Jean-Jacques, Goval
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Methylated DNA Immunoprecipitation (MeDIP)
2009Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine.
Mohn, F +3 more
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Nanobody-Based Chromatin Immunoprecipitation
2012Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale.The success of ChIP largely depends on the quality of antibodies.
NGUYEN DUC, Trong +5 more
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SDS-PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting
2012This report discusses recent methods of sample preparation and gel electrophoresis for 35S immunoprecipitation (IP) and IP western blotting. In both methods, IP is used to obtain purified proteins, and sodium dodecyl sulfate polyacrylamide gel electrophoresis is used to separate the proteins on a gel.
Edward P, Trieu, Ira N, Targoff
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Histone Native Chromatin Immunoprecipitation
2018Chromatin immunoprecipitation (ChIP) is becoming the standard method to study genome-wide distribution of histone variants and histone posttranslational modifications (PTMs). In this chapter, we describe a detailed native ChIP protocol and downstream procedures for the preparation of DNA libraries for next-generation sequencing.
Alicia, Alonso +2 more
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