Results 41 to 50 of about 1,268 (176)

DEVELOPMENT OF MUTANT HUMAN IMMUNOREACTIVE TRYPSINOGEN (IRT) FOR USE IN IMMUNOASSAYS

, 2018
In this thesis, it is aimed to develop recombinant IRT proteins that do not have a proteolytic activity to be used as a standard in Immunoreactive Trypsinogen (IRT) ELISA kits. IRT is a protein used as a marker for the diagnosis of cystic fibrosis (CF) in newborns. In addition to CF; diabetes, pancreatitis, and pancreatic cancer are possible fields for
M Divyapicigil, Ş Ş Pirinçc, T Yağci, F Yücel, E Akçael   +4 more
openaire   +3 more sources

Modifying an enzyme immunoassay of immunoreactive trypsinogen to use time-resolved fluorescence

Clinical Chemistry, 1993
Abstract A coated microtiter-well, enzyme-linked immunometric assay for quantifying immunoreactive trypsinogen in dried blood spots was modified to use time-resolved fluorescence of europium in place of end-point enzymatic color development as the quantification step.
R G, Ryall, E M, Gjerde, R L, Gerace, E, Ranieri   +3 more
openaire   +2 more sources

Cystic fibrosis: current concepts

Boletín Médico del Hospital Infantil de México, 2021
Cystic fibrosis is an autosomal recessive genetic disease, mainly in Caucasian children and young adults. It is caused by pathogenic variants in the CFTR (cystic fibrosis transmembrane conductance regulator) gene, which results in increased viscosity and
Jaime A. López-Valdez, Luis A. Aguilar-Alonso, Vanessa Gándara-Quezada, Gabriel E. Ruiz-Rico, Juana M. Ávila-Soledad, Adriana A. Reyes, Fernando D. Pedroza-Jiménez   +6 more
doaj   +1 more source

How Long are Residual Newborn Screening Specimens Useful for Retesting when Stored in Suboptimal and Uncontrolled Conditions of Temperature and Humidity?

Journal of Inborn Errors of Metabolism and Screening, 2021
Residual DBS specimens from newborns diagnosed with Phenylketonuria, Congenital Hypothyroidism, Cystic Fibrosis, Congenital Adrenal Hyperplasia and Galactosemia collected within 1995-2018, stored in cardboard boxes at ambient temperature in uncontrolled ...
Gustavo J.C. Borrajo, Vanina V. Doña
doaj   +1 more source

Determination of human pancreatic cationic trypsinogen in serum by radioimmunoassay. [PDF]

, 1979
A specific radioimmunoassay has been developed for human pancreatic cationic trypsin. The assay has been employed for the determination of immunoreactive forms of pancreatic cationic trypsin in blood.
J W Brodrick, C Largman, M C Geokas, J H Johnson   +3 more
core   +1 more source

Immunoreactive Trypsinogen Levels in Infants Born to Women with Cystic Fibrosis Taking Elexacaftor-Tezacaftor-Ivacaftor [PDF]

, 2023
Background/Objective:Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene affecting people of every race and ethnicity in the US.
Sanders, Don B., Wesson, Melissa, Chmiel, James F., Lesko, Barb, Patel, Payal, Slaven, James E., Yeley, Jana   +6 more
core   +4 more sources

Neonatal screening strategy for cystic fibrosis using immunoreactive trypsinogen and direct gene analysis [PDF]

International Journal of Gynecology & Obstetrics, 1991
To assess the effectiveness of a two tier neonatal screening strategy for cystic fibrosis, which combines estimation of immunoreactive trypsinogen followed by direct gene analysis in dried blood spot samples collected at age 5 days.Prospective study of two tier screening strategy.
E, Ranieri, R G, Ryall, C P, Morris, P V, Nelson, W F, Carey, A C, Pollard, E F, Robertson   +6 more
openaire   +2 more sources

The first five-year evaluation of cystic fibrosis neonatal screening program in São Paulo State, Brazil [PDF]

Cadernos de Saúde Pública, 2020
The Hospital of the Ribeirão Preto Medical School, University of São Paulo is one of the three screening centers in São Paulo State, Brazil, and has included a test for cystic fibrosis (CF) since February 6, 2010, by a court order. We evaluated the first
Léa Maria Zanini Maciel, Patrícia Künzle Ribeiro Magalhães, Ieda Regina Lopes Del Ciampo, Maria Luísa Barato de Sousa, Maria Inez Machado Fernandes, Regina Sawamura, Roberta Rodrigues Bittar, Greice Andreotti de Molfetta, Wilson Araújo da Silva Júnior   +8 more
doaj   +1 more source

Molecular forms of immunoreactive pancreatic cationic trypsin in pancreatitis patient sera. [PDF]

, 1979
The molecular forms of immunoreactive pancreatic cationic trypsin in sera of patients with acute pancreatic inflammation have been characterized using a radioimmunoassay technique that is capable of detecting trypsinogen as well as trypsin bound to alpha
J W Brodrick, C Largman, M Fassett, M C Geokas, J H Johnson   +4 more
core   +1 more source

The coexistence of two rare diseases thought to use the same pathologic pathway: cystic fibrosis and Niemann-Pick disease

The Turkish Journal of Pediatrics, 2022
Background. Cystic fibrosis (CF) is a multisystemic, autosomal recessive disease, which is caused by a mutation in the transmembrane conduction regulator protein (CFTR) gene.
Aslı İmran Yılmaz, Betül Uğurlu, Gökçen Ünal, Hüseyin Tokgöz, Sevgi Pekcan   +4 more
doaj   +1 more source