Results 71 to 80 of about 249,201 (263)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Low Impact Velocity Modeling of 3D Printed Spatially Graded Elastomeric Lattices. [PDF]

open access: yesPolymers (Basel), 2022
Diosdado-De la Peña JA   +6 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Response of Different Types of Chocolate to Impact Loading – Direct Impact

open access: yesActa Technologica Agriculturae
This study investigates the dynamic response of various chocolate types to direct impact at high strain rates, which has significant implications for practical applications such as production, packaging, and transportation. Five different chocolate types
Kumbár Vojtěch   +9 more
doaj   +1 more source

Analysis of key parameters and study of protective measures for wharf collision accidents

open access: yesShuili Shuiyun Gongcheng Xuebao
Ship impact has become one of the main causes of wharf damage and failure, and the damage reduces the bearing capacity and durability of the wharf structure. In order to clarify the characteristics of wharf collision accidents in China, 54 ship collision
CHEN Jifeng, LU Jun, HE Jianxin, TIAN Qi
doaj   +1 more source

Impact Velocity and Coefficient of Restitution of a Table Tennis Ball

open access: yesInternational Scholastic Journal of Science, 2022
The impact of a table tennis ball upon a paddle was recorded using a high-speed camera. The effect of the ball’s initial impact velocity on its coefficient of restitution was determined for impact velocities ranging from 1.4 ms-1 to 20.2 ms-1.
Krin Muangsiri
doaj  

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

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