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Developmental Dynamics, 2009
AbstractGene expression fields in embryogenesis are spatially precise and often small, so experimental gene expression often requires similar spatial definition. For in ovo electroporation, typically a gene construct is injected into a natural body cavity in the embryo prior to electroporation.
J E, Simkin, S J, McKeown, D F, Newgreen
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AbstractGene expression fields in embryogenesis are spatially precise and often small, so experimental gene expression often requires similar spatial definition. For in ovo electroporation, typically a gene construct is injected into a natural body cavity in the embryo prior to electroporation.
J E, Simkin, S J, McKeown, D F, Newgreen
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In ovo vaccination technology.
Advances in veterinary medicine, 1999More than 80% of the U.S. broiler industry has converted to the in ovo vaccination process for control of Marek's disease. Providing certain criteria are met, including timing and site of vaccine placement, vaccine mixing, machine sanitization, and hatchery management specifications, this has proven to be an efficacious and convenient method of ...
C A, Ricks +11 more
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2012
Electroporation has been used successfully to introduce macromolecules such as DNA into the chick embryo for at least 15 years. Purified plasmid DNA is microinjected into embryo and then a series of low voltage electrical pulses are applied to the embryo which allows naked DNA to enter cells.
Teri L, Belecky-Adams +2 more
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Electroporation has been used successfully to introduce macromolecules such as DNA into the chick embryo for at least 15 years. Purified plasmid DNA is microinjected into embryo and then a series of low voltage electrical pulses are applied to the embryo which allows naked DNA to enter cells.
Teri L, Belecky-Adams +2 more
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In ovo electroporation of avian somites
Developmental Dynamics, 2004AbstractIn ovo electroporation is a well‐established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high ...
Scaal, Martin +3 more
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In ovo sexing in poultry chicks
2023High laying performance and simultaneous high fattening performance in chicken breeding are mutually exclusive due to negative correlations. Currently, roosters from laying lines can only be raised and marketed economically to a very limited extent. Generally, the majority of male offspring are culled immediately after hatching and marketed as animal ...
Anke Förster, Thomas Bartels
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