Results 131 to 140 of about 236,648 (327)

In silico Design of Truncated Omp31 Protein of Brucella melitensis: Its Cloning and High Level Expression in Escherichia coli

Vaccine Research, 2014
Introduction: Omp31 is animmunodominant and protective antigen conserved in Brucella species and a good candidate for vaccine design. Material & methods: The present study aimed at in silico design of the truncated Omp31 (TOmp31) using bioinformatic ...
Maryam Golshani, Payam Zandi, Saeid Bouzari   +2 more
doaj  

Modeling ChIP Sequencing In Silico with Applications

PLoS Computational Biology, 2008
ChIP sequencing (ChIP-seq) is a new method for genomewide mapping of protein binding sites on DNA. It has generated much excitement in functional genomics. To score data and determine adequate sequencing depth, both the genomic background and the binding sites must be properly modeled.
Zhengdong D Zhang, Joel Rozowsky, Michael Snyder, Joseph Chang, Mark Gerstein   +4 more
openaire   +3 more sources

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Computational modeling and simulation in oncology

Clinical and Translational Discovery
Computational modeling and simulation are playing an increasingly important role in oncology, bridging biological research, data science and clinical practice to better understand cancer complexity and inform therapeutic development.
Christian Baumgartner
doaj   +2 more sources

In silico characterization and homology modeling of Nile tilapia (Oreochromis niloticus) Hsp70cBi and Hsp70cBc proteins

Heliyon
The molecular chaperone heat shock proteins 70 (Hsp70) play a pivotal role in preserving cellular integrity and managing stress. This study extensively examined two Hsp70 proteins, On-Hsp70cBi, inducible, and On-Hsp70cBc, constitutively expressed, in ...
Geraldine B. Dayrit, Normela Patricia F. Burigsay, Emmanuel M. Vera Cruz, Mudjekeewis D. Santos   +3 more
doaj   +1 more source

Modeling arterial pulse waves in healthy aging: a database for in silico evaluation of hemodynamics and pulse wave indexes

American Journal of Physiology. Heart and Circulatory Physiology, 2019
The arterial pulse wave (PW) is a rich source of information on cardiovascular (CV) health. It is widely measured by both consumer and clinical devices. However, the physical determinants of the PW are not yet fully understood, and the development of PW ...
P. Charlton, J. Mariscal Harana, S. Vennin, Ye Li, P. Chowienczyk, J. Alastruey   +5 more
semanticscholar   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Exploring the anti-inflammatory effects of bioactive compounds from assam tea clones: in silico and in vitro approaches

International Journal of Secondary Metabolite
The consumption of tea, derived from Camellia sinensis, ranks second globally after water. This research explores the anti-inflammatory properties of tea plant varieties from Assam, including TEEN ALI, TV-17, and TV-22, through a comprehensive approach ...
Hariram Natarajan, Nishanth Pitcham
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source