Results 131 to 140 of about 93,106 (305)

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

Advanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

In silico exploration of nitrogenase in Ensifer fredii unraveling structural homology functional and phylogenetic relationships and molecular dynamics

Discover Chemistry
The nitrogenase Fe protein, central to nitrogen fixation, has been extensively studied, However, the X-ray structure of the Nase-Fe protein from Ensifer fredii is essential for understanding its function and stability, yet this structure is absent from ...
Mohamed Hnini, Karim Rabeh
doaj   +1 more source

PRMT1‐Mediated LDHA Methylation Drives STAT3 Lactylation to Orchestrate Intestinal Inflammation and Tumorigenesis

Advanced Science, EarlyView.
This study identifies an immunometabolic axis wherein SAM‐driven PRMT1 methylates LDHA, enhancing its activity. The resultant lactate induces STAT3 K709 lactylation, which stabilizes an active conformation to promote STAT3 phosphorylation and IL‐10 expression.
Hui Wang, Mengyu Zhang, Weipeng Gong, Jiaxuan Wu, Junping Zhang, Wenxiu Zhang, Yue Liu, Kui Wang, Canhua Huang, Jun Zhou, Sijin Wu, Yan Li, Tianliang Li   +12 more
wiley   +1 more source

Modeling of human P450 oxidoreductase structure by in silico mutagenesis and MD simulation

, 2009
P450 oxidoreductase (POR) is the obligate electron donor for microsomal cytochrome P450s and mutations in POR cause several metabolic disorders. We have modeled the structure of human P450 oxidoreductase by in silico amino acid replacements in the rat ...
Mullis, Primus E, Pandey, Amit V, Flück, Christa E   +2 more
core   +1 more source

Computer Assisted Geometry Optimization for in silico [PDF]

Applied Medical Informatics, 2011
This paper aimed to present a methodology for the geometry optimization of molecules in order to analyze structure-activity/property relationships. The main objective was to reduce the probability of carrying out misleading analyses while preparing the ...
Lorentz JÄNTSCHI
doaj  

Cross Kingdom Metabolic Engineering Paradigm Elevating Sustainable Protein Production

Advanced Science, EarlyView.
ABSTRACT Confronting the dual crisis of escalating global protein demand and unsustainable agriculture necessitates transformative solutions. Here, we pioneer evolutionary insights from maize nitrogen optimization via asparagine synthetase (ASNS) to rewire metabolism in Pichia pastoris.
Yuanyuan Du, Changyu Pi, Kai Hong, Le Gao, Xin Wu   +4 more
wiley   +1 more source

In silico evaluation of arrhythmia

, 2017
Iteration between computational and experimental physiology has largely contributed to expanding scientific knowledge in cardiovascular research over the last decades.
Rodriguez, Blanca, Alfonso Bueno-Orovio, Rodriguez, B, Bueno Orovio, Alfonso, Zhou, X, Zhou, Xin, Bueno Orovio, A, Blanca Rodriguez, Xin Zhou   +8 more
core   +1 more source

Functional Mapping of Neurodevelopmental Disease Pathways to Key Neurodevelopmental Processes Represented in the Developmental Neurotoxicity In Vitro Testing Battery

Advanced Science, EarlyView.
Human‐relevant methods are essential for modern chemical safety assessment. This study helps define the capabilities and boundaries of an in vitro testing battery for developmental neurotoxicity by exploring its biological applicability domain. By linking neurodevelopmental disease‐related pathways to key neurodevelopmental processes, the work enhances
Eliska Kuchovska, Kristina Bartmann, Georgea Raad, Mats Schade, Luiz Ladeira, Arif Dönmez, Jördis Klose, Nicolai Görts, Denis Polozij, Lynn‐Christin Saborowski, Farina Bendt, Bernard Staumont, Liesbet Geris, Katharina Koch, Ellen Fritsche   +14 more
wiley   +1 more source

Predicting implant UHMWPE wear in-silico: A robust, adaptable computational-numerical framework for future theoretical models

, 2011
Computational methods for the pre-clinical wear prediction for devices such as hip, knee or spinal implants are valuable both to industry and academia. Archard’s wear model laid the basis for the first generation of theoretical wear estimation algorithms,
M.A. Strickland, Taylor, M., Strickland, M.A., M.R. Dressler, Dressler, M.R., M. Taylor   +5 more
core   +1 more source