Results 211 to 220 of about 33,947 (245)

Online monitoring of microcarrier based fibroblast cultivations with in situ microscopy

Biotechnology and Bioengineering, 2007
AbstractAnimal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology.
Guido, Rudolph, Patrick, Lindner, Alexander, Gierse, Arne, Bluma, Geovanni, Martinez, Bernd, Hitzmann, Thomas, Scheper   +6 more
openaire   +2 more sources

In-situ determination of sediment oxygen demand in cultivation ponds

Chinese Journal of Oceanology and Limnology, 2000
This paper presents a method for in-situ determining sediment oxygen demand (SOD) in cultivation pond. This method based on sediment surface structure, temperature, and other determining conditions like those in shrimp cultivation environments, overcomes defects of old methods and provides more accurate estimation of SOD's effect on dissolved oxygen in
Sun Yao, Chen Ju-fa, Yin Li, Song Yun-li
openaire   +1 more source

In-situ optimal control of nutrient solution for soilless cultivation

2011 3rd International Conference on Advanced Computer Control, 2011
Soilless cultivation is one of main production modes in precision agriculture. Nutrient solution for soilless cultivation primarily consists of macroelements such as NO 3 −, H 2 PO 4 −, K+, Ca2+, Mg2+, and SO 4 2−. In vegetable-nutrient solution system, there are complicated correlations between greenhouse vegetable growth and nutrient solution, thus ...
null Feng Chen, null Haidong He, null Yongning Tang   +2 more
openaire   +1 more source

In Situ Discrimination and Cultivation of Active Degraders in Soils by Genome-Directed Cultivation Assisted by SIP-Raman-Activated Cell Sorting

Environmental Science & Technology, 2023
The identification and in situ cultivation of functional yet uncultivable microorganisms are important to confirm inferences regarding their ecological functions. Here, we developed a new method that couples Raman-activated cell sorting (RACS), stable-isotope probing (SIP), and genome-directed cultivation (GDC)─namely, RACS-SIP-GDC─to identify, sort ...
Jibing Li, Dayi Zhang, Chunling Luo, Bei Li, Gan Zhang   +4 more
openaire   +2 more sources

Vortex chamber for in situ recovery of the antibiotic myxovirescin A in continuous cultivation

Biotechnology and Bioengineering, 1987
AbstractA vortex chamber for continuous adsorption of the antibiotic Myxovirescin A on XAD resins was developed. In this paper the design and use of the vortex chamber in an external bypass of a continuous process is described. Compared with the normal continuous process, the specific production rate of the antibiotic is four to five times higher when ...
V, Hecht, J, Vorlop, H, Kalbitz, K, Gerth, J, Lehmann   +4 more
openaire   +2 more sources

In situ microscopy for online monitoring of cell concentration in Pichia pastoris cultivations

Journal of Biotechnology, 2016
In situ Microscopy (ISM) is an optical non-invasive technique to monitor cells in bioprocesses in real-time. Pichia pastoris is one of the most promising protein expression systems. This yeast combines fast growth on simple media and important eukaryotic features such as glycosylation.
D. Marquard, A. Enders, G. Roth, U. Rinas, T. Scheper, P. Lindner   +5 more
openaire   +2 more sources

APPLICATION OF IN-SITU-MICROSCOPY AND DIGITAL IMAGE PROCESSING IN YEAST CULTIVATIONS

IFAC Proceedings Volumes, 2007
Abstract In-situ-microscopy is an efficient method to measure cell concentration during cultivations. Furthermore the size distribution of the cells as well as their morphology can be determined on-line. To operate the microscope and acquire sequences of cell images a software system has been developed.
P. Lindner, C. Krabichler, G. Rudolph, T. Scheper, B. Hitzmann   +4 more
openaire   +1 more source

In situ cultivation and incorporation of green manure legumes in coconut basins

Plant and Soil, 1984
Nine species of green manure legumes were tested for their ability to grow and establish in basins under coconut.Pueraria phaseoloides, Mimosa invisa andCalopogonium mucunoides were suitable yielding 19.43, 17.00 and 14.71 kg of green matter per basin.P. phaseoloides andM.
George V. Thomas, M. V. Shantaram
openaire   +1 more source

Non-destructive in situ monitoring of photosynthetic quotient in open microalgae cultivation

Bioresource Technology
Photosynthetic quotient (PQ)-based feedback strategy is emerging as a promising approach for precise optimization the cultivation of microalgae. However, the absence of in situ and non-destructive monitoring methods remains a critical bottleneck in open systems.
Yaxin, Huang, Qianfan, Zhao, Chenghu, Yan, Shumei, Wen, Zhenzhen, Zhou, Wei, Cong   +5 more
openaire   +2 more sources

Ex situ cultivation affects genetic structure and diversity in arable plants

Plant Biology, 2012
AbstractWorldwide, botanical gardens cultivate around 80,000 taxa, corresponding to approximately one‐quarter of all vascular plants. Most cultivated taxa are, however, held in a small number of collections, and mostly only in small populations. Lack of genetic exchange and stochastic processes in small populations make them susceptible to detrimental ...
C, Brütting, I, Hensen, K, Wesche
openaire   +2 more sources