Results 251 to 260 of about 37,776 (281)

In situ microscopy for online monitoring of cell concentration in Pichia pastoris cultivations

Journal of Biotechnology, 2016
In situ Microscopy (ISM) is an optical non-invasive technique to monitor cells in bioprocesses in real-time. Pichia pastoris is one of the most promising protein expression systems. This yeast combines fast growth on simple media and important eukaryotic features such as glycosylation.
D. Marquard, A. Enders, G. Roth, U. Rinas, T. Scheper, P. Lindner   +5 more
openaire   +2 more sources

Vortex chamber for in situ recovery of the antibiotic myxovirescin A in continuous cultivation

Biotechnology and Bioengineering, 1987
AbstractA vortex chamber for continuous adsorption of the antibiotic Myxovirescin A on XAD resins was developed. In this paper the design and use of the vortex chamber in an external bypass of a continuous process is described. Compared with the normal continuous process, the specific production rate of the antibiotic is four to five times higher when ...
V, Hecht, J, Vorlop, H, Kalbitz, K, Gerth, J, Lehmann   +4 more
openaire   +2 more sources

APPLICATION OF IN-SITU-MICROSCOPY AND DIGITAL IMAGE PROCESSING IN YEAST CULTIVATIONS

IFAC Proceedings Volumes, 2007
Abstract In-situ-microscopy is an efficient method to measure cell concentration during cultivations. Furthermore the size distribution of the cells as well as their morphology can be determined on-line. To operate the microscope and acquire sequences of cell images a software system has been developed.
P. Lindner, C. Krabichler, G. Rudolph, T. Scheper, B. Hitzmann   +4 more
openaire   +1 more source

In situ cultivation and incorporation of green manure legumes in coconut basins

Plant and Soil, 1984
Nine species of green manure legumes were tested for their ability to grow and establish in basins under coconut.Pueraria phaseoloides, Mimosa invisa andCalopogonium mucunoides were suitable yielding 19.43, 17.00 and 14.71 kg of green matter per basin.P. phaseoloides andM.
George V. Thomas, M. V. Shantaram
openaire   +1 more source

Non-destructive in situ monitoring of photosynthetic quotient in open microalgae cultivation

Bioresource Technology
Photosynthetic quotient (PQ)-based feedback strategy is emerging as a promising approach for precise optimization the cultivation of microalgae. However, the absence of in situ and non-destructive monitoring methods remains a critical bottleneck in open systems.
Yaxin, Huang, Qianfan, Zhao, Chenghu, Yan, Shumei, Wen, Zhenzhen, Zhou, Wei, Cong   +5 more
openaire   +2 more sources

Central Asian in situ conservation of wild relatives of cultivated plants

2000
At the present time, taxonomists, historians and botanists tend to regard Central Asia and Kazakhstan as one cohesive region from the points of view of natural history and economy, and thus they refer to the Kazakhstan—Central Asian Region, one of the largest natural regions in the world (Tikhonov and Gerasimova, 1990).
N. Lunyova, T. Ulyanova
openaire   +1 more source

Ex situ cultivation affects genetic structure and diversity in arable plants

Plant Biology, 2012
AbstractWorldwide, botanical gardens cultivate around 80,000 taxa, corresponding to approximately one‐quarter of all vascular plants. Most cultivated taxa are, however, held in a small number of collections, and mostly only in small populations. Lack of genetic exchange and stochastic processes in small populations make them susceptible to detrimental ...
C, Brütting, I, Hensen, K, Wesche
openaire   +2 more sources

In‐situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation

Biotechnology and Bioengineering, 2003
AbstractThe use of in‐situ near infrared spectroscopy (NIRS) as a tool for monitoring four key analytes in a CHO‐K1 animal cell culture was investigated. Previous work using on‐line NIRS to monitor bioprocesses has involved its application ex‐situ where the analyzer is physically outside the fermentor, or to microbial bioprocesses.
Arnold, S. Alison, Crowley, J., Woods, N., Harvey, L.M., McNeil, B.   +4 more
openaire   +4 more sources

Novel bioreactor-system for in Situ-cultivation of artificial tissue

2017
A bioreactor is a device simulating physiological environments for different biotechnological applications. In highly promising research fields like tissue engineering microsized bioreactors were utilized successfully promoting mammalian cells to grow and build 3D cell structures similar to in vivo environments.
Kostova J., Schneider S., Sauer S., Bohme A., Casalboni M., Foitzik A. H.   +5 more
openaire   +1 more source

Long-Term Yeast Cultivation Coupled with In Situ Extraction for High Triterpenoid Production

Journal of Agricultural and Food Chemistry
Ginsenosides are a class of triterpenoids from the ginseng genus, with many medicinal properties. Traditionally, ginsenosides are extracted from ginseng plants to satisfy market demand; however, this approach requires substantial plant biomass and a lengthy six-year growth period before harvest.
Shangkun Qiu, Lars M. Blank
openaire   +2 more sources