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Fluorescence In Situ Hybridization [PDF]
Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. It can visualize specific cytogenetic abnormalities (copy number aberrations) such as chromosomal deletion, amplification, and translocation. FISH has been used in prenatal diagnosis and has served both as
Amy Y.-Y. Chen, Andrew Chen
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Multiple fluorescence in situ hybridization [PDF]
AbstractA method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue.This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation ...
M. van der Ploeg+6 more
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Fluorescence in situ hybridization in hematology
nema
Ivana Milosevic+2 more
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Fluorescent in Situ Hybridization
Abstract not availableBangladesh J Med Biochem 2017; 10(1): 3 ...
S Akhter, HB Asad, Z Aziz
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Autofluorescence correction for fluorescence in situ hybridization [PDF]
AbstractOptimal sensitivity of fluorescence in situ hybridization (FISH) requires bright signals and low background fluorescence. Use of locus‐specific probes is especially dependent on high sensitivity. Some tissue preparations show high autofluorescence, masking small or dim signals.
Szöllősi, János+3 more
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Fluorescence In Situ Hybridization Probe Preparation
The public human genome sequencing project utilized a hierarchical approach. A large number of BAC/PAC clones, with an insert size approximate from 50 kb to 300 kb, were identified and finely mapped with respect to the Sequence Tagged Site (STS) physical map and with respect to each other.
TOLOMEO, DORON+2 more
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Recent Advances in Fluorescence in situ Hybridization
Fluorescence in situ hybridization (FISH) procedures that directly couple molecular and cytological information allow precise visualization of DNA sequences on metaphase chromosomes and interphase nuclei. These techniques can be used to identify chromosomes, detect chromosomal aberrations, and analyze linear and spatial genome organization.
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Fluorescence in Situ Hybridization in Pathology
Özben Yalçın, Gamze Kulduk
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Fluorescence In Situ Hybridization
2021Fluorescence in situ hybridization (FISH) is a method of using fluorescent probes to detect specific nucleic acid sequences within cells and their location. It can be used to detect a variety of cytogenetic variations, including chromosomal deletions, amplification, and translocation.
Min Hu, Weimin Wu
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Fluorescence In Situ Hybridization
2003In situ hybridization describes the annealing of a labeled nucleic acid to complementary nucleic acid sequences in a fixed target (e.g., chromosomes, free nuclei, nuclei in tissue sections, and DNA) followed by visualisation of the location of the probe.
Rachel L. Ostroff, Barbara A. Tate
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