Fluorescence in situ hybridization in hematology [PDF]
nema
Ivana Milosevic+2 more
openaire +3 more sources
Fluorescence In Situ Hybridization [PDF]
Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. It can visualize specific cytogenetic abnormalities (copy number aberrations) such as chromosomal deletion, amplification, and translocation. FISH has been used in prenatal diagnosis and has served both as
Amy Y.-Y. Chen, Andrew Chen
openaire +3 more sources
Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]
Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single ...
Jalocha, A.+5 more
core +3 more sources
FISH molecular testing in cytological preparations from solid tumors [PDF]
Many of the exciting new developments in solid tumor molecular cytogenetics impact classical and molecular pathology. Fluorescence in situ hybridization to identify specific DNA target sequences in nuclei of non-dividing cells in solid neoplasms has ...
CARIA, PAOLA, VANNI, ROBERTA
core +1 more source
Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson+15 more
core +1 more source
Localization of the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to 8q22 by in situ hybridization [PDF]
We have localized the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to chromosome 8 using both radioactive in situ hybridization and fluorescence in situ hybridization.
Heng, HHQ+4 more
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Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Anastasi+33 more
core +1 more source
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke+33 more
core +1 more source
Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization [PDF]
Comparative genomic in situ hybridization (CGH) provides a new possibility for searching genomes for imbalanced genetic material. Labeled genomic test DNA, prepared from clinical or tumor specimens, is mixed with differently labeled control DNA prepared ...
Cremer, Thomas+9 more
core +1 more source
Effect of native gastric mucus on in vivo hybridization therapies directed at Helicobacter pylori [PDF]
Helicobacter pylori infects more than 50% of the worldwide population. It is mostly found deep in the gastric mucus lining of the stomach, being a major cause of peptic ulcers and gastric adenocarcinoma. To face the increasing resistance of H.
Azevedo, Nuno F+13 more
core +5 more sources