Results 271 to 280 of about 2,801,410 (319)

Fluorescent In Situ Hybridization

2008
In situ hybridization (ISH) technique was introduced by Gall and Pardue1 in 1969. At that time the technique was limited by the use of radioactively labelled probes that were subsequently visualized by autoradiography. The development of interphase cytogenetics in the 1980s and fluorescent labels in 19862 has seen the technology applied in a number of ...
Fiona Campbell, John M. S. Bartlett
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Probe Labeling and Fluorescence In Situ Hybridization [PDF]

Current Protocols in Cytometry, 1997
AbstractThis unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post‐hybridization washes. Support protocols for probe labeling cover probe purification and quality control.
A.K. Raap, J. Wiegant
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Fluorescence In Situ Hybridization

2005
Dr. Seuss’s eloquent “One FISH, two FISH, red FISH, blue FISH” (1) could have been describing one of the most significant advancements in clinical cytogenetics, fluorescence in situ hybridization (FISH). The process, as described by Pinkel et al. in 1988 (2), involved fluorescent detection of probe DNA hybridized to chromosomal target sequences.
Stuart Schwartz, Stuart Schwartz, Daynna J. Wolff   +2 more
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Fluorescence in situ hybridization: Uses and limitations

Seminars in Hematology, 2000
The development of molecular hybridization techniques such as fluorescence in situ hybridization (FISH) has had a major Impact on efforts to detect and characterize the genetic changes that give rise to human tumors. With probes designed to Identify specific chromosomes and chromosomal regions, FISH is used routinely by cytogenetics and pathology ...
Gozzetti, A, Le Beau, M M
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Fluorescent In Situ Hybridization (FISH)

2021
In situ hybridization (ISH) is a technique that uses nucleic acid probes to allow for visualization of nucleic acid sequences in cellular/tissue preparations. Both enzymatic and fluorescent ISH studies can be used as adjunct studies, and in some cases are vital to making specific diagnoses. This chapter will focus on fluorescent in situ hybridization.
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Fluorescent In Situ Hybridization

2001
Fluorescence in situ hybridization (FISH) arose from a marriage of classical DNA hybridization in solution to modern molecular biologic techniques, most notably the use of restriction endonucleases and, later, the polymerase chain reaction (PCR). The former has made identification of both genes and relevant interspersed sequences possible, whereas ...
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Fluorescence In situ Hybridization

1996
During the last decade, pathology has progressed remarkably with the incorporation of molecular techniques into the arena of diagnostics. Fluorescence in situ hybridization (FISH) is a molecular histopathological technique that can be readily used to identify DNA or RNA abnormalities at the cellular level with the use of an epifluorescence microscope ...
Victoria Belogolovkin, Victoria Belogolovkin, Jerry A. Varkey, Jerry A. Varkey, Charles T. Ladoulis, Sunny Luke, Sunny Luke   +6 more
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Fluorescence In Situ Hybridization (FISH)

Current Protocols in Cell Biology, 2004
AbstractFluorescence in situ Hybridization (FISH) involves the preparation of two main components: the DNA probe and the target DNA to which the probe will be hybridized. The DNA probe typically comes from cloned sources such as plasmids, cosmids, PACs, YACs, or BACs; where the insert may contain a specific gene or originate from a specific chromosomal
Jeremy A. Squire, Jane Bayani
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Raman-Fluorescence in Situ Hybridization

2014
As with many other molecular microbiology methods, the family of stable isotope probing (SIP) techniques based on the analysis of isotope- labeled nucleic acids, phospholipid fatty acids (PLFAs), or proteins are frequently performed at the level of the community.
Read, Daniel, Whiteley, Andrew
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Advances in fluorescence in situ hybridization

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1998
The techniques of in situ hybridization (ISH) are widely applied for analyzing the genetic make-up and RNA expression patterns of individual cells. This review focusses on a number of advances made over the last 5 years in the fluorescence ISH (FISH) field, i.e., Fiber-FISH, Multi-colour chromosome painting, Comparative Genomic Hybridization, Tyramide ...
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