Results 21 to 30 of about 2,801,410 (319)

Real-Time Monitoring of Fluorescence in situ Hybridization (FISH) Kinetics [PDF]

published 2018, 2018
We present a novel method for real-time monitoring and kinetic analysis of fluorescence in situ hybridization (FISH). We implement the method using a vertical microfluidic probe containing a microstructure designed for rapid switching between a probe solution and a non-fluorescent imaging buffer.
arxiv   +1 more source

HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization

Indian Journal of Pathology and Microbiology, 2012
Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH).
Hemlata S Shirsat, Sridhar Epari, Tanuja Shet, Rajani Bagal, Rohini Hawaldar, Sangeeta B Desai   +5 more
doaj   +1 more source

A simplified and rapid in situ hybridization protocol for planarians

BioTechniques, 2023
Whole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting ...
Andrew J Gaetano, Ryan S King
doaj   +1 more source

Fluorescence in situ hybridization improves the detection of 5q31 deletion in myelodysplastic syndromes without cytogenetic evidence of 5q-

Haematologica, 2008
Background More than 50% of patients with myelodysplastic syndromes present cytogenetic aberrations at diagnosis. Partial or complete deletion of the long arm of chromosome 5 is the most frequent abnormality.
Mar Mallo, Leonor Arenillas, Blanca Espinet, Marta Salido, Jesús Mª Hernández, Eva Lumbreras, Mónica del Rey, Eva Arranz, Soraya Ramiro, Patricia Font, Olga González, Mónica Renedo, José Cervera, Esperanza Such, Guillermo F. Sanz, Elisa Luño, Carmen Sanzo, Miriam González, María José Calasanz, José Mayans, Carlos García-Ballesteros, Victoria Amigo, Rosa Collado, Isabel Oliver, Félix Carbonell, Encarna Bureo, Andrés Insunza, Lucrecia Yañez, María José Muruzabal, Elena Gómez-Beltrán, Rafael Andreu, Pilar León, Valle Gómez, Ángeles Sanz, Natalia Casasola, Esperanza Moreno, Adrián Alegre, María Luisa Martín, Carmen Pedro, Sergi Serrano, Lourdes Florensa, Francesc Solé   +41 more
doaj   +1 more source

Multiple fluorescence in situ hybridization [PDF]

Cytometry, 1990
AbstractA method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue.This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation ...
M. van der Ploeg, Joop Wiegant, S. van der Flier, Petra M. Nederlof, Johan S. Ploem, Anton K. Raap, H. J. Tanke   +6 more
openaire   +3 more sources

In Situ Hybridization Pada Kanker Payudara [PDF]

, 2014
Kesulitan yang dijumpai pada penanganan kanker payudara adalah terjadinya kekambuhan atau relaps. Deteksi status HER2 pada pasien merupakan salah satu upaya untuk mendeteksi terjadinya relaps dan juga untuk menentukan jenis terapi yang ada diberikan ...
Diah Witari, Ni Putu
core   +2 more sources

Ion track reconstruction in 3D using alumina-based fluorescent nuclear track detectors [PDF]

Physics in Medicine and Biology 58 (2013), N251, 2013
Fluorescent nuclear track detectors (FNTDs) based on Al2O3:C,Mg single crystal combined with confocal microscopy provide 3D information on ion tracks with a resolution only limited by light diffraction. FNTDs are also ideal substrates to be coated with cells to engineer cell-fluorescent ion track hybrid detectors.
arxiv   +1 more source

Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets [PDF]

, 1992
Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes.
Cremer, Thomas, Popp, Susanne
core   +1 more source

Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes [PDF]

, 1988
Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines ...
A.H.N. Hopman, Atkin, Bigner, Brigati, Burns, Burns, Carine, Cavenee, Comings, Cooke, Cooke, Cremer, D. Tesin, Dale, Devilee, Devilee, Dudin, Durnam, Gosden, Hadlaczky, Hall, Henn, Higgins, Hopman, Hopman, Hopman, Hopman, Hopman, Joseph, Julien, Kinzler, Kohl, L. Manuelidis, Landegent, Landegent, Langer-Safer, Leary, Lund Jorgensen, Maniatis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, McDermid, Müller, Müncke, Pinkel, Pinkel, Rappold, Schardin, Schwab, Shapiro, Siegel, T. Cremer, Therman, Trask, Willard, Wu, Yang   +64 more
core   +1 more source

Fluorescence in situ Hybridization (FISH) in the Microbiological Diagnostic of Deep Sternal Wound Infection (DSWI)

Infection and Drug Resistance, 2021
Nick Spindler,1 Annette Moter,2,3 Alexandra Wiessner,2,3 Tanja Gradistanac,4 Michael Borger,5 Arne C Rodloff,6 Stefan Langer,1 Judith Kikhney2,3 1Department of Orthopedic Surgery, Traumatology and Plastic Surgery, University Hospital Leipzig, Leipzig ...
Spindler N, Moter A, Wiessner A, Gradistanac T, Borger M, Rodloff AC, Langer S, Kikhney J   +7 more
doaj