Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations
Highly selective chemistries are required for fabrication and post‐cross–linking modification of cell‐encapsulating hydrogels used in tissue engineering applications. Isonitrile ligation reactions represent a promising class of bioorthogonal chemistries for engineering hydrogel‐based cellular microenvironments. Isonitrile‐based hydrogels are stable and
Ping Zhou+2 more
wiley +1 more source
Fluorescence In Situ Hybridization Method for Measuring Transfection Efficiency
We describe here the use of fluorescence in situ hybridization (FISH) to measure the transfection efficiency of the transient expression vector pCMVcat in lymphoblasts and fibroblasts.
Lie Cheng, Corazon D. Bucana, Qingyi Wei
doaj +1 more source
Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide
Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
doaj +1 more source
Sorting of chromosomes by magnetic separation [PDF]
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
A Oberteufer+30 more
core +1 more source
Fluorine‐functionalized organic spacers (2F, 3F, 5F) are investigated to identify as optimal passivation agents for 3D MAPI perovskite solar cells (PSCs). The study highlights how altering fluorine positioning affects defect passivation, low‐dimensional phase (LDP) formation, and stability, emphasizing its role in molecular interactions and offering ...
Ali Semerci+15 more
wiley +1 more source
A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
A Kallioniemi+31 more
core +1 more source
Structured, Shaped, or Printed Single‐Atom Catalysts and Their Applications
This paper reviews the design and use of structured single‐atom catalysts, which integrate porous architectures with the exceptional reactivity of isolated catalytic sites. It explores fabrication strategies, advanced characterization methods, and support materials that enhance thermal stability, mechanical robustness, and operational efficiency of ...
Jiachengjun Luo+4 more
wiley +1 more source
A Fluorescent Ratiometric Potassium Sensor Based on IPG4-Silica Microparticles for Selective Detection and Fluorescence Imaging of Potassium Cations [PDF]
Potassium cations play many important roles in living organisms, especially in electro-physiology, since they are involved in neurotransmission and muscle contractions. We report the synthesis of a ratiometric fluorescent microsensor for potassium (K+) detection, based on the fluorescent probe ION potassium green 4.
arxiv +1 more source
Aneusomy of chromosomes 7 and 17 predicts the recurrence of transitional cell carcinoma of the urinary bladder [PDF]
Objective To determine if changes in chromosome 7 and 17 copy number can be used to predict recurrence in patients with primary noninvasive (pTa) or superficially invasive (pT1) transitional cell carcinoma (TCC) of the urinary bladder.
A.D. Watters+26 more
core +1 more source
Sono‐Piezo‐Photosynthesis of Ethylene and Acetylene from Bioethanol under Ambient Conditions
A novel sono‐piezo‐photocatalytic strategy enables the conversion of bioethanol to ethylene (C2H4) and acetylene (C2H2) under ambient conditions, addressing the limitations of conventional methods that are high‐temperature and energy‐intensive. By using an advanced graphene oxide/sodium bismuth titanate heterostructure catalyst, this work achieved ...
Yue Jiang+21 more
wiley +1 more source