Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide
Cells, 2021 Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
doaj +1 more source
A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]
, 2009 Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Cremer, M. +4 more
core +1 more source
Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization [PDF]
, 1993 A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p−, and a 21q+. These derivative chromosomes could not be defined by GTG-banding.
Anna Jauch +26 more
core +1 more source
A pipeline for targeted metagenomics of environmental bacteria. [PDF]
, 2020 BackgroundMetagenomics and single cell genomics provide a window into the genetic repertoire of yet uncultivated microorganisms, but both methods are usually taxonomically untargeted.
Bowers, Robert M +7 more
core +2 more sources
Semantic segmentation of mFISH images using convolutional networks [PDF]
, 2018 Multicolor in situ hybridization (mFISH) is a karyotyping technique used to detect major chromosomal alterations using fluorescent probes and imaging techniques. Manual interpretation of mFISH images is a time consuming step that can be automated using machine learning; in previous works, pixel or patch wise classification was employed, overlooking ...
arxiv +1 more source
PointFISH -- learning point cloud representations for RNA localization patterns [PDF]
arXiv, 2023 Subcellular RNA localization is a critical mechanism for the spatial control of gene expression. Its mechanism and precise functional role is not yet very well understood. Single Molecule Fluorescence in Situ Hybridization (smFISH) images allow for the detection of individual RNA molecules with subcellular accuracy.
arxiv
miniaturized fish for screening of onco hematological malignancies [PDF]
, 2010 Fluorescence in situ hybridization (FISH) represents a major step in the analysis of chromosomal aberrations in cancer. It allows the precise detection of specific rearrangements, both for diagnost..
Andrea Zanardi +7 more
core +1 more source
A Fluorescent Ratiometric Potassium Sensor Based on IPG4-Silica Microparticles for Selective Detection and Fluorescence Imaging of Potassium Cations [PDF]
Journal of Materials Chemistry B 2024,10573Potassium cations play many important roles in living organisms, especially in electro-physiology, since they are involved in neurotransmission and muscle contractions. We report the synthesis of a ratiometric fluorescent microsensor for potassium (K+) detection, based on the fluorescent probe ION potassium green 4.
arxiv +1 more source
Translocation (8;21) in acute nonlymphocytic leukemia delineated by chromosomal in situ suppression hybridization [PDF]
, 1991 In situ suppression hybridization with recombinant bacteriophage DNA libraries for chromosomes 8 and 21 was performed in two cases of acute nonlymphocytic leukemia, type FAB M2.
Anna Jauch +23 more
core +1 more source
An interpretable automated detection system for FISH-based HER2 oncogene amplification testing in histo-pathological routine images of breast and gastric cancer diagnostics [PDF]
arXiv, 2020 Histo-pathological diagnostics are an inherent part of the everyday work but are particularly laborious and associated with time-consuming manual analysis of image data. In order to cope with the increasing diagnostic case numbers due to the current growth and demographic change of the global population and the progress in personalized medicine ...
arxiv