Results 71 to 80 of about 121,743 (264)
MethodsX, 2019 A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward +2 more
doaj
A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
Science and Technology of Advanced Materials, 2016 We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target,
Hiromu Kashida +2 more
doaj +1 more source
Advanced Healthcare Materials, EarlyView.This study develops 3D‐printed Mg‐MC/PLGA scaffolds with varying Mg concentrations (0–20%). The 5% Mg scaffold shows optimal cytocompatibility, osteogenic activity in vitro, and significantly enhances bone regeneration in rabbits, improving bone volume and mechanical strength.
Shihang Liu +9 more
wiley +1 more source
Fluorescence In Situ Hybridization
FISH and the related in situ hybridization (ISH) techniques provide complementary genomic information to qPCR, ddPCR, NGS, and NanoString techniques. This technology was initially developed as a physical mapping tool to delineate genes within chromosomes. Its high analytical resolution to a single gene level and high sensitivity and specificity enables
openaire +1 more source
Advanced Healthcare Materials, EarlyView.Granular hydrogels are emerging microporous platforms for cell culture and delivery, showing great potential for replicating the complex, heterogeneous environments found in natural tissues. This review outlines the design principles of granular hydrogels, highlighting critical factors that determine the final physicochemical properties of the entire ...
Shuhan Feng, Kaiyang Chen, Shiqi Wang
wiley +1 more source
Roll‐To‐Roll Production of Smart Dressings for Wound Monitoring
Advanced Healthcare Materials, EarlyView.This study presents a roll‐to‐roll manufactured smart wound dressing capable of visually monitoring key wound biomarkers, including pH, humidity, and temperature. By integrating colorimetric sensors into flexible dressing substrates, the device offers real‐time, equipment‐free insights for infection detection and wound management, representing a ...
Ziheng Wang +14 more
wiley +1 more source
Self‐Assembling Multi‐Antigen T Cell Hybridizers for Precision Immunotherapy of Multiple Myeloma
Advanced Healthcare Materials, EarlyView.A novel modular T‐cell engager platform, MATCH, is developed for personalized multiple myeloma therapy. MATCH uses complementary oligonucleotide‐linked Fab’ fragments to enable flexible targeting of MM antigens and controlled T‐cell activation. The system shows potent, antigen‐specific cytotoxicity in vitro and in vivo, highlighting its potential for ...
Shannuo Li +8 more
wiley +1 more source
Printing and Rerouting of Elastic and Protease Responsive Shape Memory Hydrogel Filaments
Advanced Healthcare Materials, EarlyView.A biofabrication strategy, Rerouting of Free‐Floating Suspended Hydrogel Filaments (REFRESH), is developed enabling the printing of highly elastic, reconfigurable protease‐responsive hydrogel filaments with shape memory properties. The filaments can be manually braided, knotted, and rerouted to create intricate cell‐laden 3D architectures, expanding ...
Philip Lifwergren +10 more
wiley +1 more source
Synthetic Aspects and Characterization Needs in MOF Chemistry – from Discovery to Applications
Advanced Materials, EarlyView.Overcoming the challenges of phase discovery, synthesis optimization and scale‐up, characterization, and computational studies is essential to accelerate the large‐scale application of MOFs. Life‐cycle analyses and techno‐economic analyses need to be performed to realistically assess their potential for industrial relevance.
Bastian Achenbach +4 more
wiley +1 more source
MethodsX, 2019 DNA-fluorescence in situ hybridisation (DNA-FISH) allows visualisation of chromosome organisation and rearrangement. FISH probes are pools of short fluorescently labelled DNA fragments that are often produced from template plasmids that contain large ...
Patrick J. McCoy +4 more
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