M-FISH Karyotyping - A New Approach Based on Watershed Transform [PDF]
Karyotyping is a process in which chromosomes in a dividing cell are properly stained, identified and displayed in a standard format, which helps geneticist to study and diagnose genetic factors behind various genetic diseases and for studying cancer. M-FISH (Multiplex Fluorescent In-Situ Hybridization) provides color karyotyping.
arxiv +1 more source
Longitudinal study on clinical and microbial analysis of periodontal status in pregnancy
This study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (FISH) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks ...
Fernanda Campos MACHADO+4 more
doaj +1 more source
Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics [PDF]
DNA libraries from sorted human gonosomes were used selectively to stain the X and Y chromosomes in normal and aberrant cultured human cells by chromosomal in situ suppression (CISS-) hybridization.
Cremer, Thomas+5 more
core +1 more source
Clumped Nuclei Segmentation with Adjacent Point Match and Local Shape based Intensity Analysis for Overlapped Nuclei in Fluorescence In-Situ Hybridization Images [PDF]
Highly clumped nuclei clusters captured in fluorescence in situ hybridization microscopy images are common histology entities under investigations in a wide spectrum of tissue-related biomedical investigations. Due to their large scale in presence, computer based image analysis is used to facilitate such analysis with improved analysis efficiency and ...
arxiv
Fishing for Digeorge Syndrome in A 40-YEAR-OLD Man
: Objective: Identify an endocrine developmental syndrome (DiGeorge syndrome, or 22q11 deletion syndrome) that had been previously undiagnosed in an adult patient.Methods: The patient was evaluated in an adult endocrinology clinic.
Christopher J. Hupfeld, MD+2 more
doaj
Upper tract urothelial carcinomas in the proximal ureter are an uncommon disease. We present a case in which it was firstly detected by fluorescence in situ hybridization and not by endoscopy and radiologic imaging.
Guoxian Zhang+5 more
doaj +1 more source
Differences of size and shape of active and inactive X-chromosome domains in human amniotic fluid cell nuclei [PDF]
It is a widely held belief that the inactive X-chromosome (Xi) in female cell nuclei is strongly condensed as compared to the largely decondensed active X-chromosome (Xa).
Barr+39 more
core +1 more source
The cholesterol transfer function of StAR is uniquely integrated into adrenal cells, with mRNA translation and PKA phosphorylation occurring at the mitochondrial outer membrane (OMM).
Jinwoo Lee+5 more
doaj +1 more source
Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries [PDF]
The nuclear topography of splicing snRNPs, mRNA transcripts and chromosome domains in various mammalian cell types are described. The visualization of splicing snRNPs, defined by the Sm antigen, and coiled bodies, revealed distinctly different ...
A Monneron+64 more
core +1 more source
Mutually enhancing light emission between plasmonic nanostructures and fluorescent emitters [PDF]
We demonstrate that the fluorescent emitters can increase light emission from the plasmonic nanostructures in turn. With the help of atomic force microscopy, a hybrid system consisting of a fluorescent nanodiamond and a gold nanoparticle was assembled step-by-step for in situ optical measurements.
arxiv