Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries [PDF]
A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported.
B Trask+39 more
core +1 more source
Detection of papillomavirus DNA in sity hybridization technique was perfomed in 29 symptomatic patients (6 males and 23 females) during the period of 1989-1991 at the Clinic for Sexually Transmitted Diseases, Universidade Federal Fluminense, State of rio
L. H. S. Oliveira+4 more
doaj +1 more source
Infantile nodular fasciitis of the hand: A case report and literature review
Pediatric nodular fasciitis is uncommon and has a preference for the head and neck region. Occurrence in other anatomic locations is uncommon. We describe here a case of nodular fasciitis that arose in the hand of a newborn infant who presented with a ...
I Weng Lao+3 more
doaj +1 more source
We established a sensitive non-radioactive in situ hybridization (ISH) method for the detection of chicken IgG &ggr;-chain mRNA in paraffin sections. RNA probes were transcribed in vitro from cloned chicken IgG CH1 nucleotide sequences with SP6/T7 RNA ...
Zheng Weiming+3 more
doaj +1 more source
Comparison of ethylene carbonate and formamide as components of the hybridization mixture in FISH
The protocols for in situ hybridization (ISH) techniques can vary considerably; however, they usually include denaturation and hybridization steps. Denaturing compounds are used to reduce denaturation and hybridization temperature, which keeps the proper
Anna Kalinka+2 more
doaj +1 more source
Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson+15 more
core +1 more source
A modified protocol for the detection of three different mRNAs with a new-generation in situ hybridization chain reaction on frozen sections [PDF]
A new multiple fluorescence in situ hybridization method based on hybridization chain reaction was recently reported, enabling simultaneous mapping of multiple target mRNAs within intact zebrafish and mouse embryos.
Burnstock, G+7 more
core
Comparative study of in situ and ex situ MgB2 films deposited by pulsed laser deposition [PDF]
Two types of MgB2 films were prepared by pulsed laser deposition (PLD) with in situ and ex situ annealing processes respectively. Significant differences in properties between the two types of films were found. The ex situ MgB2 film has a Tc of 38.1K, while the in situ film has a depressed Tc of 34.5K.
arxiv +1 more source
Localization of the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to 8q22 by in situ hybridization [PDF]
We have localized the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to chromosome 8 using both radioactive in situ hybridization and fluorescence in situ hybridization.
Heng, HHQ+4 more
core +1 more source
Understanding the Impact of Synchronous, Asynchronous, and Hybrid In-Situ Techniques in Computational Fluid Dynamics Applications [PDF]
High-Performance Computing (HPC) systems provide input/output (IO) performance growing relatively slowly compared to peak computational performance and have limited storage capacity. Computational Fluid Dynamics (CFD) applications aiming to leverage the full power of Exascale HPC systems, such as the solver Nek5000, will generate massive data for ...
arxiv +1 more source