Results 61 to 70 of about 370,888 (338)

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

STAR Protocols, 2021
Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development.
Rajendra Kumar Gurumurthy, Naveen Kumar, Cindrilla Chumduri   +2 more
doaj  

An Examination of Aerosol Jet‐Printed Surface Roughness and its Impact on the Performance of High‐Frequency Electronics

Advanced Engineering Materials, EarlyView.
This study explores aerosol jet‐printed (AJP) surface roughness, its effects on the performance of microwave electronics, and its process contributors. First, an electromagnetic model is vetted for AJP's unique roughness signature. Simulations are built which show process‐induced roughness is as significant as conductor resistivity in driving microwave
Christopher Areias, Alkim Akyurtlu
wiley   +1 more source

Double-staining chromogenic in situ hybridization as a useful alternative to split-signal fluorescence in situ hybridization in lymphoma diagnostics

Haematologica, 2010
Background Malignant lymphomas are classified based on morphology, immunophenotype, genetics and clinical features. The pathological diagnosis is generally considered difficult and prone to mistakes.
Anke van Rijk, Tim Svenstroup-Poulsen, Margaret Jones, José Cabeçadas, Juan Cruz Cigudosa, Lorenzo Leoncini, Anja Mottok, Christiane Copie Bergman, Evi Pouliou, Stephen Hamilton Dutoit, Han J. van Krieken   +10 more
doaj   +1 more source

Recent Progress on 2D‐Material‐Based Smart Textiles: Materials, Methods, and Multifunctionality

Advanced Engineering Materials, EarlyView.
Advancements in 2D‐material‐integrated smart textiles are reviewed, with a focus on materials, fabrication methods, and multifunctional applications, including energy harvesting, monitoring, EMI shielding, energy storage, and thermal management. The discussion addresses key challenges and provides insights into the future development of next‐generation
Yong Choi, Jiheon Kim, Jaemin Lee, Xinqi Chen, Byungseok Seo, Wonjoon Choi   +5 more
wiley   +1 more source

Tribology of ZrC Coatings and Spherical Tips Acquired with Extraordinarily Stiff, Metal Atomic Force Microscopy Probes

Advanced Engineering Materials, EarlyView.
In this research, ZrC coatings are evaluated against various counterprobes at the microscale using novel super‐stiff atomic force microscopy cantilevers. The chemical composition of the coating is shown to be an important factor influencing coating hardness and Young's modulus, while surface roughness, counterprobe hardness, and surface energy are the ...
Piotr Jenczyk, Marcin Michałowski, Bogdan Warcholiński, Michał Milczarek, Adam Gilewicz   +4 more
wiley   +1 more source

Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue

STAR Protocols, 2023
Summary: Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive.
Rachel Warren, Aftab Shaik, Lauren Teubner, Handeng Lyu, Stijn De Langhe   +4 more
doaj  

Dual-color dual-hapten in situ hybridization (D-DISH) – Comparison with fluorescence in situ hybridization (FISH) for HER2/neu testing in breast cancer

Indian Journal of Pathology and Microbiology, 2020
Context: HER2/neu testing in breast cancer is a mandate due to availability of trastuzumab, a monoclonal antibody targeted against this biomarker.
Pooja K Gajaria, Sonali Tambe, Trupti Pai, Asawari Patil, Sangeeta B Desai, Tanuja M Shet   +5 more
doaj   +1 more source

Thermokinetic Modeling Approach for Aluminide Diffusion Coatings on Molybdenum Substrates

Advanced Engineering Materials, EarlyView.
The advancement of innovative materials for protective coatings in high‐temperature environments relies on a detailed understanding of diffusion processes within these systems. This article presents findings from modeling approaches, supporting the development and optimization of coating materials by focusing on the prediction of diffusion‐driven ...
Jurica Filipovic, Katharina Beck, Christian Kontermann, Mathias Galetz, Matthias Oechsner   +4 more
wiley   +1 more source

Quantification of virus-infected cells using RNA FISH-Flow

STAR Protocols, 2023
Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren, Arturo Barbachano-Guerrero, Devra Huey, Qing Yang, Emma R. Worden-Sapper, Jens H. Kuhn, Sara L. Sawyer   +6 more
doaj  

Fluorescence In Situ Hybridization Method for Co-Localization of mRNA and GFP

BioTechniques, 2001
Co-localization studies using green fluorescent protein (GFP) and fluorescence immunohistochemistry have become commonplace. However, co-localization studies using GFP and mRNA in situ hybridization are rare, in large part because typical in situ ...
Anthony A. Oliva, John W. Swann
doaj   +1 more source