Results 51 to 60 of about 297,554 (311)

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Technical Note: Determination of the metabolically active fraction of benthic foraminifera by means of Fluorescent In Situ Hybridization (FISH) [PDF]

, 2011
Benthic foraminifera are an important component of the marine biota, but protocols for investigating their viability and metabolism are still extremely limited.
Negri, Alessandra, Danovaro, R., Luna, G, M, Nardelli, M., Negri A., Morigi, C., C. Morigi, Sabbatini A, Nardelli MP, Borrelli, C, Sabbatini, A., Borrelli C, Luna GM, Luna, Gian Marco, Sbaffi, T, M.P. Nardelli, Danovaro R, R. Danovaro, A. Sabbatini, G.M. Luna, Borrelli, Chiara, Luna, G,, Sabbatini, Anna, Negri, A, M. P. Nardelli, Nardelli, Maria Pia, Morigi, Caterina, Danovaro, Roberto, A. Negri, G. M. Luna, T. Sbaffi, C. Borrelli, Sbaffi T, Sbaffi, Tomasa   +33 more
core   +1 more source

Expression of Kisspeptin 1 in the Brain of the Adult Sea Lamprey Petromyzon marinus

Life, 2021
Kisspeptin peptides play major roles in the regulation of reproduction and puberty onset in mammals. While most mammals only have one kisspeptin gene, other jawed vertebrates present two or three genes. Recent data also revealed the presence of two genes
Daniel Sobrido-Cameán, Luis Alfonso Yáñez-Guerra, Alexandre Deber, María Celina Rodicio, Antón Barreiro-Iglesias   +4 more
doaj   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

The origin of human chromosome 2 analyzed by comparative chromosome mapping with a DNA microlibrary [PDF]

, 1994
Fluorescencein situ hybridization (FISH) of microlibraries established from distinct chromosome subregions can test the evolutionary conservation of chromosome bands as well as chromosomal rearrangements that occurred during primate evolution and will ...
Cremer, Thomas, Senger, G., Wienberg, Johannes, Claussen, U., Horsthemke, B., Arnold, N., Lüdecke, H. J., Jauch, Anna, Lengauer, Christoph   +8 more
core   +1 more source

Protocol for detection of glial complement expression in relation to amyloid plaques in mouse brain with combined FISH and IHC

STAR Protocols
Summary: Complement proteins contribute to neurodegeneration in Alzheimer’s disease (AD) and are secreted by glia surrounding beta-amyloid (Aβ) plaques.
Sadhna Rao, Joanna Yung, Melissa Gonzalez Edick, Jesse E. Hanson   +3 more
doaj   +1 more source

Analysis of target mRNAs in the fixed-frozen human brain using a modified BaseScope-ISH Assay protocol

STAR Protocols, 2022
Summary: We describe a modified BaseScope™ Assay protocol (ACDBio) for RNA in situ hybridization on fixed-frozen human brain tissue. The original protocol caused tissue detachment due to harsh tissue pre-treatment.
M. Carla Carisì, Owain W. Howell, Alwena H. Morgan, Jeffrey S. Davies   +3 more
doaj   +1 more source

Towards Quantitative In Situ Hybridization [PDF]

Journal of Histochemistry & Cytochemistry, 1997
In situ hybridization analysis of tissue mRNA concentrations remains to be accepted as a quantitative technique, even though exposure of tissue sections to photographic emulsion is equivalent to Northern blot analysis. Because of the biological importance of in situ quantification of RNA sequences within a morphological context, we
Jonker, A., de Boer, P.A.J., van den Hoff, M.J.B., Lamers, W.H., Moorman, A.F.M.   +4 more
openaire   +5 more sources

Complementarity of Long‐Reads and Optical Mapping in Parkinson's Disease for Structural Variants

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Long‐read sequencing and optical genome mapping technologies have the ability to detect large and complex structural variants. This has led to the discovery of novel pathogenic variants in neurodegenerative movement disorders. Thus, we aimed to systematically compare the SV detection capabilities of OGM and ONT in Parkinson's disease.
André Fienemann, Theresa Lüth, Susen Schaake, Carolin Gabbert, Marius Möller, Hauke Busch, Katja Lohmann, Jonas A. Gustafson, Danny E. Miller, Kensuke Daida, Manabu Funayama, Nobutaka Hattori, Samia Ben Sassi, Faycel Hentati, Matthew J. Farrer, Kristian K. Ullrich, Christine Klein, Joanne Trinh   +17 more
wiley   +1 more source

Quantification and analysis of multiplexed fluorescence in situ hybridization data using open-source tools

STAR Protocols
Summary: Here, we present a protocol to quantify and analyze multiplexed fluorescence in situ hybridization (mFISH) data using two open-source tools, FijiFISH and RUHi.
Kaitlin E. Sullivan, Margarita Kapustina, Brianna N. Bristow, Mark S. Cembrowski   +3 more
doaj   +1 more source