A Novel Amplification Method of Nonradioactive In Situ Hybridization Signal for Specific RNA with Biotinylated Tyramine [PDF]
For a better performance of nonradioactive in situ hybridization for specific RNA sequences, signal amplification is sometimes required especially in the use of clinical specimens processed under suboptimal conditions and in the analysis of gene ...
Hoshino, Akiko +7 more
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In Situ Hybridization Status of 568 Cases of HER2 Equivocal Breast Cancer
Objective To compare the differences between 2013 and 2018 ASCO/CAP HER2 testing guidelines in 568 immunohistochemical HER2 equivocal breast cancer patients and to analyze the accuracy and deficiency of dual-color silver enhanced in-situ hybridization ...
JIANG Yi'na +3 more
doaj +1 more source
Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka +8 more
doaj +1 more source
Fluorescence in situ hybridization to chromosomes as a tool to understand human and primate genome evolution [PDF]
For the last 15 years molecular cytogenetic techniques have been extensively used to study primate evolution. Molecular probes were helpful to distinguish mammalian chromosomes and chromosome segments on the basis of their DNA content rather than solely ...
Wienberg, Johannes, Wienberg, J.
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Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]
We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Kilian, Karin +6 more
core +1 more source
Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets [PDF]
Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes.
Cremer, Thomas, Popp, Susanne
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Distant hybridization enhances interspecific gene flow, facilitating emergence of novel species and polyploid lineages. However, investigating the genetic evolutionary relationships between distantly hybridized vertebrate offspring of different ploidy ...
Yalan Zhang +16 more
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A high-resolution anatomical framework of the neonatal mouse brain for managing gene expression data
This study aims to provide a high-resolution atlas and use it as an anatomical framework to localize the gene expression data for mouse brain on postnatal day 0 (P0).
Jyl Boline, Arthur W Toga
doaj +1 more source
Fluorescence in situ hybridization in hematology [PDF]
nema
Milošević Ivana +2 more
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Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridization
Summary: Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections.
Yunming Wu, C. Ron Yu
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