Results 61 to 70 of about 114,296,728 (302)

In situ hybridization

Molecular and Cellular Probes, 1987
In situ hybridization is the hybridization-mediated detection of specific nucleic acid sequences within structurally intact cells or tissues. As such it uniquely provides localization of nucleic acid superimposed on observable cellular and subcellular structural detail, allowing analysis unobtainable by other hybridization techniques.
openaire   +2 more sources

PANoptosis in the pathogenesis of myelodysplastic syndromes

Molecular Oncology, EarlyView.
PANoptosis, a combination of three types of programmed cell death, is mediated by a large protein complex called a PANoptosome. In healthy bone marrow hematopoietic cells, PANoptosis is restricted by inhibitory signaling. In MDS, bone marrow cells become sensitive to the PANoptotic stimuli due to the aberrant inactivation of inhibitory signaling or ...
Rohit Thalla, Jyoti Lamichhane, Cameron Lewis, Peter Breslin, Jiwang Zhang   +4 more
wiley   +1 more source

Technical Note: Determination of the metabolically active fraction of benthic foraminifera by means of Fluorescent In Situ Hybridization (FISH) [PDF]

, 2011
Benthic foraminifera are an important component of the marine biota, but protocols for investigating their viability and metabolism are still extremely limited.
Negri, Alessandra, Danovaro, R., Luna, G, M, Nardelli, M., Negri A., Morigi, C., C. Morigi, Sabbatini A, Nardelli MP, Borrelli, C, Sabbatini, A., Borrelli C, Luna GM, Luna, Gian Marco, Sbaffi, T, M.P. Nardelli, Danovaro R, R. Danovaro, A. Sabbatini, G.M. Luna, Borrelli, Chiara, Luna, G,, Sabbatini, Anna, Negri, A, M. P. Nardelli, Nardelli, Maria Pia, Morigi, Caterina, Danovaro, Roberto, A. Negri, G. M. Luna, T. Sbaffi, C. Borrelli, Sbaffi T, Sbaffi, Tomasa   +33 more
core   +2 more sources

Towards Quantitative In Situ Hybridization [PDF]

Journal of Histochemistry & Cytochemistry, 1997
In situ hybridization analysis of tissue mRNA concentrations remains to be accepted as a quantitative technique, even though exposure of tissue sections to photographic emulsion is equivalent to Northern blot analysis. Because of the biological importance of in situ quantification of RNA sequences within a morphological context, we
Jonker, A., de Boer, P.A.J., van den Hoff, M.J.B., Lamers, W.H., Moorman, A.F.M.   +4 more
openaire   +5 more sources

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

Protocol for detection of glial complement expression in relation to amyloid plaques in mouse brain with combined FISH and IHC

STAR Protocols
Summary: Complement proteins contribute to neurodegeneration in Alzheimer’s disease (AD) and are secreted by glia surrounding beta-amyloid (Aβ) plaques.
Sadhna Rao, Joanna Yung, Melissa Gonzalez Edick, Jesse E. Hanson   +3 more
doaj   +1 more source

Expression of Kisspeptin 1 in the Brain of the Adult Sea Lamprey Petromyzon marinus

Life, 2021
Kisspeptin peptides play major roles in the regulation of reproduction and puberty onset in mammals. While most mammals only have one kisspeptin gene, other jawed vertebrates present two or three genes. Recent data also revealed the presence of two genes
Daniel Sobrido-Cameán, Luis Alfonso Yáñez-Guerra, Alexandre Deber, María Celina Rodicio, Antón Barreiro-Iglesias   +4 more
doaj   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]

, 1993
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
Schindler, Detlev, Speicher, Michael R., Riethman, Harold C., Cremer, Thomas, Donis-Keller, Helen, Popp, Susanne, Jauch, Anna, Lengauer, Christoph   +7 more
core   +1 more source

Quantification and analysis of multiplexed fluorescence in situ hybridization data using open-source tools

STAR Protocols
Summary: Here, we present a protocol to quantify and analyze multiplexed fluorescence in situ hybridization (mFISH) data using two open-source tools, FijiFISH and RUHi.
Kaitlin E. Sullivan, Margarita Kapustina, Brianna N. Bristow, Mark S. Cembrowski   +3 more
doaj   +1 more source