In Situ Hybridization Status of 568 Cases of HER2 Equivocal Breast Cancer
Objective To compare the differences between 2013 and 2018 ASCO/CAP HER2 testing guidelines in 568 immunohistochemical HER2 equivocal breast cancer patients and to analyze the accuracy and deficiency of dual-color silver enhanced in-situ hybridization ...
JIANG Yi'na +3 more
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In situ hybridization is the hybridization-mediated detection of specific nucleic acid sequences within structurally intact cells or tissues. As such it uniquely provides localization of nucleic acid superimposed on observable cellular and subcellular structural detail, allowing analysis unobtainable by other hybridization techniques.
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Fluorescence In Situ Hybridization [PDF]
Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. It can visualize specific cytogenetic abnormalities (copy number aberrations) such as chromosomal deletion, amplification, and translocation. FISH has been used in prenatal diagnosis and has served both as
Chen, Amy Y.-Y., Chen, Andrew
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Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]
We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Kilian, Karin +6 more
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Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka +8 more
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Molecular and classical cytogenetic analyses demonstrate an apomorphic reciprocal chromosomal translocation in Gorilla gorilla [PDF]
The existence of an apomorphic reciprocal chromosomal translocation in the gorilla lineage has been asserted or denied by various cytogeneticists. We employed a new molecular cytogenetic strategy (chromosomal in situ suppression hybridization) combined ...
Stanyon, Roscoe +11 more
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Distant hybridization enhances interspecific gene flow, facilitating emergence of novel species and polyploid lineages. However, investigating the genetic evolutionary relationships between distantly hybridized vertebrate offspring of different ploidy ...
Yalan Zhang +16 more
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A high-resolution anatomical framework of the neonatal mouse brain for managing gene expression data
This study aims to provide a high-resolution atlas and use it as an anatomical framework to localize the gene expression data for mouse brain on postnatal day 0 (P0).
Jyl Boline, Arthur W Toga
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Fluorescence in situ hybridization in hematology [PDF]
nema
Milošević Ivana +2 more
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Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridization
Summary: Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections.
Yunming Wu, C. Ron Yu
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