Results 41 to 50 of about 458,373 (262)

A high-resolution anatomical framework of the neonatal mouse brain for managing gene expression data

Frontiers in Neuroinformatics, 2007
This study aims to provide a high-resolution atlas and use it as an anatomical framework to localize the gene expression data for mouse brain on postnatal day 0 (P0).
Jyl Boline, Arthur W Toga
doaj   +1 more source

Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridization

STAR Protocols, 2022
Summary: Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections.
Yunming Wu, C. Ron Yu
doaj   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Protocol for detection of glial complement expression in relation to amyloid plaques in mouse brain with combined FISH and IHC

STAR Protocols
Summary: Complement proteins contribute to neurodegeneration in Alzheimer’s disease (AD) and are secreted by glia surrounding beta-amyloid (Aβ) plaques.
Sadhna Rao, Joanna Yung, Melissa Gonzalez Edick, Jesse E. Hanson   +3 more
doaj   +1 more source

Expression of Kisspeptin 1 in the Brain of the Adult Sea Lamprey Petromyzon marinus

Life, 2021
Kisspeptin peptides play major roles in the regulation of reproduction and puberty onset in mammals. While most mammals only have one kisspeptin gene, other jawed vertebrates present two or three genes. Recent data also revealed the presence of two genes
Daniel Sobrido-Cameán, Luis Alfonso Yáñez-Guerra, Alexandre Deber, María Celina Rodicio, Antón Barreiro-Iglesias   +4 more
doaj   +1 more source

Localization of the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to 8q22 by in situ hybridization [PDF]

, 1996
We have localized the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to chromosome 8 using both radioactive in situ hybridization and fluorescence in situ hybridization.
Heng, HHQ, Malaney, S, Robinson, BH, Shi, XM, Tsui, LC   +4 more
core   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

Quantification and analysis of multiplexed fluorescence in situ hybridization data using open-source tools

STAR Protocols
Summary: Here, we present a protocol to quantify and analyze multiplexed fluorescence in situ hybridization (mFISH) data using two open-source tools, FijiFISH and RUHi.
Kaitlin E. Sullivan, Margarita Kapustina, Brianna N. Bristow, Mark S. Cembrowski   +3 more
doaj   +1 more source

Analysis of target mRNAs in the fixed-frozen human brain using a modified BaseScope-ISH Assay protocol

STAR Protocols, 2022
Summary: We describe a modified BaseScope™ Assay protocol (ACDBio) for RNA in situ hybridization on fixed-frozen human brain tissue. The original protocol caused tissue detachment due to harsh tissue pre-treatment.
M. Carla Carisì, Owain W. Howell, Alwena H. Morgan, Jeffrey S. Davies   +3 more
doaj   +1 more source

Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries [PDF]

, 1992
We established chromosomal homologies between all chromosomes of the human karyotype and that of an old world monkey (Macaca fuscata) by chromosomal in situ suppression (CISS) hybridization with human chromosome specific DNA libraries.
Cremer, Thomas, Jauch, Anna, Stanyon, Roscoe, Wienberg, Johannes   +3 more
core   +1 more source