Results 21 to 30 of about 458,373 (262)

Imaging of RNA in situ hybridization by atomic force microscopy [PDF]

, 1996
In this study we investigated the possibility of imaging internal cellular molecules after cytochemical detection with atomic force microscopy (AFM). To this end, rat 9G and HeLa cells were hybridized with haptenized probes for 28S ribosomal RNA, human ...
Corput, M.P.C. van de, Grooth, B.G. de, Kalle, W.H.J., Macville, M.V.E., Raap, A.K., Tanke, H.J.   +5 more
core   +3 more sources

Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers

Biological Procedures Online, 2004
In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription ...
Gerhart Jacquelyn, Baytion Michael, Perlman Jordanna, Neely Christine, Hearon Bridget, Nilsen Thor, Getts Robert, Kadushin James, George-Weinstein Mindy   +8 more
doaj   +1 more source

Significance of molecular diagnostics in human papilloma virus (HPV) determination [PDF]

Archives of Biological Sciences, 2014
HPV infection is considered to be the most important etiologic factor in cervical cancer development. In this retrospective study, which included the period from 2000 to 2012, the results of two molecular techniques used in the detection of HPV ...
Kovačević G., Milošević V., Hrnjaković-Cvjetković I., Petrović V., Stefan-Mikić S., Radovanov J., Jovanović-Galović A., Patić A., Elez I.   +8 more
doaj   +1 more source

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

STAR Protocols, 2021
Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development.
Rajendra Kumar Gurumurthy, Naveen Kumar, Cindrilla Chumduri   +2 more
doaj   +1 more source

Genome composition of 'Elatior'-begonias hybrids analyzed by genomic in situ hybridisation [PDF]

, 2010
Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called 'Elatior'-begonias hybrids (B. x hiemalis Fotsch).
Laak, W.A., Marasek Ciolakowska, A.R., Ramanna, M.S., Tuyl, J.M., van   +3 more
core   +2 more sources

Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

Biology Open, 2012
Summary Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression
Anne Karine Lagendijk, Jon D. Moulton, Jeroen Bakkers   +2 more
doaj   +1 more source

Quantification of virus-infected cells using RNA FISH-Flow

STAR Protocols, 2023
Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren, Arturo Barbachano-Guerrero, Devra Huey, Qing Yang, Emma R. Worden-Sapper, Jens H. Kuhn, Sara L. Sawyer   +6 more
doaj   +1 more source

Study of pregnant women with high risk of fetus abnormalities by routine cytogenetics method (karyotyping) and molecular method (FISH) by using X and Y probs and comparing the advantages and disadvantages of these methods in the northwest of Iran's patients [PDF]

مجله پزشکی دانشگاه علوم پزشکی تبریز, 2021
Background: The health of the fetus during the 9 months of pregnancy is very important for every pregnant couple. Identifying carriers of the genetic diseases and their diagnosis before birth, controls the disease's prevalence and does not impose huge ...
Parisa Vahidi, Seyed Ali Rahmani, Nahid Hadige Rezvan   +2 more
doaj   +1 more source

Chromosomal in situ suppression hybridization of immunologically classified mitotic cells in hematologic malignancies [PDF]

, 1992
Chromosomal in situ suppression (CISS) hybridization was performed with library DNA from sorted human chromosomes 8, 9, 15, 17, 21, and 22 on immunologically stained bone marrow cells of four patients with a hematologic neoplasm, including two patients ...
Arnoldus, Cremer, Cremer, Dauwerse, Edwards, Erber, Hopman, Johnson, Jotterand Bellomo, Knuutila, Knuutila, Larramendy, Larramendy, Lichter, Lichter, Marianne Tiainen, Martine Jotterand Bellomo, Patricia Emmerich, Perez Losada, Pinkel, Pinkel, Popp, Sakari Knuutila, Susanne Popp, Tapani Ruutu, Teerenhovi, Thomas Cremer, Valerie Parlier, Wessman, Willard   +29 more
core   +1 more source

Fluorescent in situ hybridization (FISH) on the veterinary diagnostic field

Acta Fytotechnica et Zootechnica, 2016
Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+) was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative
Ján Dianovský, Beáta Holečková, Monika Drážovská, Katarína Šivíková, Viera Schwarzbacherová   +4 more
doaj   +1 more source