Results 21 to 30 of about 297,554 (311)

Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization [PDF]

open access: yes, 1990
Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes
Cremer, Thomas   +4 more
core   +1 more source

Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]

open access: yes, 1992
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Thomas Ried   +23 more
core   +1 more source

Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization

open access: yesJournal of Veterinary Internal Medicine, 2023
Background Follicular cystitis is an uncommon inflammatory change in the urinary bladder wall characterized by the formation of tertiary lymphoid structures (TLSs) in the submucosa.
Sanna J. Viitanen   +4 more
doaj   +1 more source

Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers

open access: yesBiological Procedures Online, 2004
In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription ...
Gerhart Jacquelyn   +8 more
doaj   +1 more source

Significance of molecular diagnostics in human papilloma virus (HPV) determination [PDF]

open access: yesArchives of Biological Sciences, 2014
HPV infection is considered to be the most important etiologic factor in cervical cancer development. In this retrospective study, which included the period from 2000 to 2012, the results of two molecular techniques used in the detection of HPV ...
Kovačević G.   +8 more
doaj   +1 more source

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

open access: yesSTAR Protocols, 2021
Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development.
Rajendra Kumar Gurumurthy   +2 more
doaj   +1 more source

Study of pregnant women with high risk of fetus abnormalities by routine cytogenetics method (karyotyping) and molecular method (FISH) by using X and Y probs and comparing the advantages and disadvantages of these methods in the northwest of Iran's patients [PDF]

open access: yesمجله پزشکی دانشگاه علوم پزشکی تبریز, 2021
Background: The health of the fetus during the 9 months of pregnancy is very important for every pregnant couple. Identifying carriers of the genetic diseases and their diagnosis before birth, controls the disease's prevalence and does not impose huge ...
Parisa Vahidi   +2 more
doaj   +1 more source

Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization [PDF]

open access: yes, 1993
Comparative genomic in situ hybridization (CGH) provides a new possibility for searching genomes for imbalanced genetic material. Labeled genomic test DNA, prepared from clinical or tumor specimens, is mixed with differently labeled control DNA prepared ...
Speicher, Michael R.   +9 more
core   +1 more source

Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

open access: yesBiology Open, 2012
Summary Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression
Anne Karine Lagendijk   +2 more
doaj   +1 more source

Quantification of virus-infected cells using RNA FISH-Flow

open access: yesSTAR Protocols, 2023
Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren   +6 more
doaj   +1 more source

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