Results 21 to 30 of about 240,787 (262)

Multimodal mapping of cell types and projections in the central nucleus of the amygdala

open access: yeseLife, 2023
The central nucleus of the amygdala (CEA) is a brain region that integrates external and internal sensory information and executes innate and adaptive behaviors through distinct output pathways. Despite its complex functions, the diversity of molecularly
Yuhan Wang   +10 more
doaj   +1 more source

In situ detection of mature microRNAs by labeled extension on ultramer templates

open access: yesBioTechniques, 2009
We describe a new method for the in situ detection of a mature microRNA (miRNA) in formalin-fixed, paraffin-embedded tissues. The method involves the labeled extension of miRNA hybridized to an approximately 100-nucleotide–long ultramer template ...
Gerard J. Nuovo   +4 more
doaj   +1 more source

Identification of Mucormycosis by Fluorescence In Situ Hybridization Targeting Ribosomal RNA in Tissue Samples

open access: yesJournal of Fungi, 2022
Mucormycosis is an invasive fungal infection associated with high mortality, partly due to delayed diagnosis and inadequate empiric therapy. As fungal cultures often fail to grow Mucorales, identification of respective hyphae in tissue is frequently ...
Jill Jasmine Dalimot   +7 more
doaj   +1 more source

Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization

open access: yesJournal of Veterinary Internal Medicine, 2023
Background Follicular cystitis is an uncommon inflammatory change in the urinary bladder wall characterized by the formation of tertiary lymphoid structures (TLSs) in the submucosa.
Sanna J. Viitanen   +4 more
doaj   +1 more source

Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers

open access: yesBiological Procedures Online, 2004
In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription ...
Gerhart Jacquelyn   +8 more
doaj   +1 more source

Significance of molecular diagnostics in human papilloma virus (HPV) determination [PDF]

open access: yesArchives of Biological Sciences, 2014
HPV infection is considered to be the most important etiologic factor in cervical cancer development. In this retrospective study, which included the period from 2000 to 2012, the results of two molecular techniques used in the detection of HPV ...
Kovačević G.   +8 more
doaj   +1 more source

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

open access: yesSTAR Protocols, 2021
Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development.
Rajendra Kumar Gurumurthy   +2 more
doaj   +1 more source

Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

open access: yesBiology Open, 2012
Summary Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression
Anne Karine Lagendijk   +2 more
doaj   +1 more source

Quantification of virus-infected cells using RNA FISH-Flow

open access: yesSTAR Protocols, 2023
Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren   +6 more
doaj   +1 more source

Fluorescent in situ hybridization (FISH) on the veterinary diagnostic field

open access: yesActa Fytotechnica et Zootechnica, 2016
Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+) was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative
Ján Dianovský   +4 more
doaj   +1 more source

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