Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84 [PDF]
The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84.
Pearson, P. +17 more
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Fluorescent in situ hybridization (FISH) on the veterinary diagnostic field
Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+) was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative
Ján Dianovský +4 more
doaj +1 more source
Summary: Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive.
Rachel Warren +4 more
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It has long been known that armored scale insects harbor endosymbiotic bacteria inside specialized cells called bacteriocytes. Originally, these endosymbionts were thought to be fungal symbionts but they are now known to be bacterial and have been named ...
Katharina Dittmar +2 more
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Background Triple-negative breast carcinoma (TNBC) patients do not benefit from hormone- or human epidermal growth factor receptor 2- (HER2-) targeted therapies. Accurate testing is pivotal for these patients. Methods TNBC cases that were retested at our
Jose De Jesus, Marilin Rosa
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Differential Analysis of Active and Inactive Genes in Human Neutrophils by Chromosomal In Situ Hybridization [PDF]
In situ hybridization has been widely used for localization of specific genes in interphase cell nuclei. The sites of signals, however, do not represent the activity of the gene transcription in the nuclei.
Masashi Shin +9 more
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Morphological detection of X- and Y-chromosomes in smears and paraffin-embedded tissues using a non-isotopic in situ hybridization technique (NISH) [PDF]
Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which ...
Eisenmenger, Wolfgang +4 more
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A Novel Amplification Method of Nonradioactive In Situ Hybridization Signal for Specific RNA with Biotinylated Tyramine [PDF]
For a better performance of nonradioactive in situ hybridization for specific RNA sequences, signal amplification is sometimes required especially in the use of clinical specimens processed under suboptimal conditions and in the analysis of gene ...
Hoshino, Akiko +7 more
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In Situ Gene Expression in Native Cryofixed Bone Tissue
Bone is a very complex tissue that is constantly changing throughout the lifespan. The precise mechanism of bone regeneration remains poorly understood.
Krisztina Nikovics +6 more
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Fluorescence in situ hybridization to chromosomes as a tool to understand human and primate genome evolution [PDF]
For the last 15 years molecular cytogenetic techniques have been extensively used to study primate evolution. Molecular probes were helpful to distinguish mammalian chromosomes and chromosome segments on the basis of their DNA content rather than solely ...
Wienberg, Johannes, Wienberg, J.
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