Results 31 to 40 of about 240,787 (262)

Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue

STAR Protocols, 2023
Summary: Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive.
Rachel Warren, Aftab Shaik, Lauren Teubner, Handeng Lyu, Stijn De Langhe   +4 more
doaj   +1 more source

Distribution of the Primary Endosymbiont (Candidatus Uzinura Diaspidicola) Within Host Insects from the Scale Insect Family Diaspididae

Insects, 2012
It has long been known that armored scale insects harbor endosymbiotic bacteria inside specialized cells called bacteriocytes. Originally, these endosymbionts were thought to be fungal symbionts but they are now known to be bacterial and have been named ...
Katharina Dittmar, Meghan Flarhety, Matthew E. Gruwell   +2 more
doaj   +1 more source

Study of pregnant women with high risk of fetus abnormalities by routine cytogenetics method (karyotyping) and molecular method (FISH) by using X and Y probs and comparing the advantages and disadvantages of these methods in the northwest of Iran's patients [PDF]

مجله پزشکی دانشگاه علوم پزشکی تبریز, 2021
Background: The health of the fetus during the 9 months of pregnancy is very important for every pregnant couple. Identifying carriers of the genetic diseases and their diagnosis before birth, controls the disease's prevalence and does not impose huge ...
Parisa Vahidi, Seyed Ali Rahmani, Nahid Hadige Rezvan   +2 more
doaj   +1 more source

Suboptimal concordance in testing and retesting results of triple-negative breast carcinoma cases among laboratories: one institution experience

Cancer Cell International, 2019
Background Triple-negative breast carcinoma (TNBC) patients do not benefit from hormone- or human epidermal growth factor receptor 2- (HER2-) targeted therapies. Accurate testing is pivotal for these patients. Methods TNBC cases that were retested at our
Jose De Jesus, Marilin Rosa
doaj   +1 more source

In Situ Gene Expression in Native Cryofixed Bone Tissue

Biomedicines, 2022
Bone is a very complex tissue that is constantly changing throughout the lifespan. The precise mechanism of bone regeneration remains poorly understood.
Krisztina Nikovics, Cédric Castellarin, Xavier Holy, Marjorie Durand, Halima Morin, Abdelhafid Bendahmane, Anne-Laure Favier   +6 more
doaj   +1 more source

In Situ Hybridization Status of 568 Cases of HER2 Equivocal Breast Cancer

Zhongliu Fangzhi Yanjiu, 2019
Objective To compare the differences between 2013 and 2018 ASCO/CAP HER2 testing guidelines in 568 immunohistochemical HER2 equivocal breast cancer patients and to analyze the accuracy and deficiency of dual-color silver enhanced in-situ hybridization ...
JIANG Yi'na, WANG Bo, ZHOU Can, WANG Hongyan   +3 more
doaj   +1 more source

Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization

Frontiers in Molecular Neuroscience, 2022
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka, Yusuke Atsumi, Yusuke Atsumi, Aki Makanae, Aki Makanae, Mitsuru Yashiro, Mitsuru Yashiro, Hiromasa Funato, Hiromasa Funato   +8 more
doaj   +1 more source

In situ hybridization

Molecular and Cellular Probes, 1987
In situ hybridization is the hybridization-mediated detection of specific nucleic acid sequences within structurally intact cells or tissues. As such it uniquely provides localization of nucleic acid superimposed on observable cellular and subcellular structural detail, allowing analysis unobtainable by other hybridization techniques.
openaire   +2 more sources

Fluorescence In Situ Hybridization [PDF]

Journal of Investigative Dermatology, 2013
Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. It can visualize specific cytogenetic abnormalities (copy number aberrations) such as chromosomal deletion, amplification, and translocation. FISH has been used in prenatal diagnosis and has served both as
Chen, Amy Y.-Y., Chen, Andrew
openaire   +2 more sources

Revealing chromosomal loci and compositional variations in different ploidy lineages of common carp (♀) × blunt snout bream (♂) via in situ hybridization

Aquaculture Reports
Distant hybridization enhances interspecific gene flow, facilitating emergence of novel species and polyploid lineages. However, investigating the genetic evolutionary relationships between distantly hybridized vertebrate offspring of different ploidy ...
Yalan Zhang, Lan Chen, Hanyu Liu, Yi Fan, Wei Chen, Sijin Fan, Yu Hu, Xinyue Deng, Gaode Zhong, Huan Zhong, Fangzhou Hu, Ming Wen, Wuhui Li, Chang Wu, Qizhi Liu, Shi Wang, Shaojun Liu   +16 more
doaj   +1 more source