Results 31 to 40 of about 458,373 (262)

Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue

open access: yesSTAR Protocols, 2023
Summary: Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive.
Rachel Warren   +4 more
doaj   +1 more source

Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]

open access: yes, 1992
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Anastasi   +33 more
core   +1 more source

Distribution of the Primary Endosymbiont (Candidatus Uzinura Diaspidicola) Within Host Insects from the Scale Insect Family Diaspididae

open access: yesInsects, 2012
It has long been known that armored scale insects harbor endosymbiotic bacteria inside specialized cells called bacteriocytes. Originally, these endosymbionts were thought to be fungal symbionts but they are now known to be bacterial and have been named ...
Katharina Dittmar   +2 more
doaj   +1 more source

Suboptimal concordance in testing and retesting results of triple-negative breast carcinoma cases among laboratories: one institution experience

open access: yesCancer Cell International, 2019
Background Triple-negative breast carcinoma (TNBC) patients do not benefit from hormone- or human epidermal growth factor receptor 2- (HER2-) targeted therapies. Accurate testing is pivotal for these patients. Methods TNBC cases that were retested at our
Jose De Jesus, Marilin Rosa
doaj   +1 more source

Molecular and classical cytogenetic analyses demonstrate an apomorphic reciprocal chromosomal translocation in Gorilla gorilla [PDF]

open access: yes, 1992
The existence of an apomorphic reciprocal chromosomal translocation in the gorilla lineage has been asserted or denied by various cytogeneticists. We employed a new molecular cytogenetic strategy (chromosomal in situ suppression hybridization) combined ...
Bigoni, F.   +5 more
core   +1 more source

In Situ Gene Expression in Native Cryofixed Bone Tissue

open access: yesBiomedicines, 2022
Bone is a very complex tissue that is constantly changing throughout the lifespan. The precise mechanism of bone regeneration remains poorly understood.
Krisztina Nikovics   +6 more
doaj   +1 more source

Role of dorsomedial striatum neuronal ensembles in incubation of methamphetamine craving after voluntary abstinence [PDF]

open access: yes, 2017
We recently developed a rat model of incubation of methamphetamine craving after choice-based voluntary abstinence. Here, we studied the role of dorsolateral striatum (DLS) and dorsomedial striatum (DMS) in this incubation.
Bossert, Jennifer M.   +6 more
core   +2 more sources

In Situ Hybridization Status of 568 Cases of HER2 Equivocal Breast Cancer

open access: yesZhongliu Fangzhi Yanjiu, 2019
Objective To compare the differences between 2013 and 2018 ASCO/CAP HER2 testing guidelines in 568 immunohistochemical HER2 equivocal breast cancer patients and to analyze the accuracy and deficiency of dual-color silver enhanced in-situ hybridization ...
JIANG Yi'na   +3 more
doaj   +1 more source

Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization

open access: yesFrontiers in Molecular Neuroscience, 2022
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka   +8 more
doaj   +1 more source

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]

open access: yes, 1992
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson   +15 more
core   +1 more source

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