In situ visualization of bacterial populations in coral tissues: pitfalls and solutions [PDF]
PeerJ, 2016 In situ visualization of microbial communities within their natural habitats provides a powerful approach to explore complex interactions between microorganisms and their macroscopic hosts.
Naohisa Wada +5 more
doaj +2 more sources
Chromosomal in situ suppression hybridization of immunologically classified mitotic cells in hematologic malignancies [PDF]
, 1992 Chromosomal in situ suppression (CISS) hybridization was performed with library DNA from sorted human chromosomes 8, 9, 15, 17, 21, and 22 on immunologically stained bone marrow cells of four patients with a hematologic neoplasm, including two patients ...
Knuutila, Sakari +15 more
core +1 more source
Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries [PDF]
, 1991 A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphase chromosomes of man and primates including ...
Cremer, Thomas +4 more
core +1 more source
Indian Journal of Pathology and Microbiology, 2012 Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH).
Hemlata S Shirsat +5 more
doaj +1 more source
In situ hybridization in paracoccidioidomycosis [PDF]
Medical Mycology, 1999 In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence.
T, De Brito +7 more
openaire +2 more sources
Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
, 1992 Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Cremer, Thomas +2 more
core +1 more source
Multimodal mapping of cell types and projections in the central nucleus of the amygdala
eLife, 2023 The central nucleus of the amygdala (CEA) is a brain region that integrates external and internal sensory information and executes innate and adaptive behaviors through distinct output pathways. Despite its complex functions, the diversity of molecularly
Yuhan Wang +10 more
doaj +1 more source
Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization [PDF]
, 1993 A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p−, and a 21q+. These derivative chromosomes could not be defined by GTG-banding.
Speicher, Michael R. +5 more
core +1 more source
In situ detection of mature microRNAs by labeled extension on ultramer templates
BioTechniques, 2009 We describe a new method for the in situ detection of a mature microRNA (miRNA) in formalin-fixed, paraffin-embedded tissues. The method involves the labeled extension of miRNA hybridized to an approximately 100-nucleotide–long ultramer template ...
Gerard J. Nuovo +4 more
doaj +1 more source
Journal of Fungi, 2022 Mucormycosis is an invasive fungal infection associated with high mortality, partly due to delayed diagnosis and inadequate empiric therapy. As fungal cultures often fail to grow Mucorales, identification of respective hyphae in tissue is frequently ...
Jill Jasmine Dalimot +7 more
doaj +1 more source