Results 71 to 80 of about 2,969,693 (257)

Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract

STAR Protocols, 2021
Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development.
Rajendra Kumar Gurumurthy, Naveen Kumar, Cindrilla Chumduri   +2 more
doaj  

Chromosomal in situ suppression hybridization of immunologically classified mitotic cells in hematologic malignancies [PDF]

, 1992
Chromosomal in situ suppression (CISS) hybridization was performed with library DNA from sorted human chromosomes 8, 9, 15, 17, 21, and 22 on immunologically stained bone marrow cells of four patients with a hematologic neoplasm, including two patients ...
Arnoldus, Cremer, Cremer, Dauwerse, Edwards, Erber, Hopman, Johnson, Jotterand Bellomo, Knuutila, Knuutila, Larramendy, Larramendy, Lichter, Lichter, Marianne Tiainen, Martine Jotterand Bellomo, Patricia Emmerich, Perez Losada, Pinkel, Pinkel, Popp, Sakari Knuutila, Susanne Popp, Tapani Ruutu, Teerenhovi, Thomas Cremer, Valerie Parlier, Wessman, Willard   +29 more
core   +1 more source

Double-staining chromogenic in situ hybridization as a useful alternative to split-signal fluorescence in situ hybridization in lymphoma diagnostics

Haematologica, 2010
Background Malignant lymphomas are classified based on morphology, immunophenotype, genetics and clinical features. The pathological diagnosis is generally considered difficult and prone to mistakes.
Anke van Rijk, Tim Svenstroup-Poulsen, Margaret Jones, José Cabeçadas, Juan Cruz Cigudosa, Lorenzo Leoncini, Anja Mottok, Christiane Copie Bergman, Evi Pouliou, Stephen Hamilton Dutoit, Han J. van Krieken   +10 more
doaj   +1 more source

Síndrome de Williams: proposta de sistema de pontuação para diagnóstico clínico [PDF]

, 2007
OBJECTIVE: To develop a scoring system based on clinical findings to assist pediatricians in the diagnosis of William syndrome and to delineate when the fluorescent in-situ hybridization test to detect the microdeletion at 7q11.23 may be needed. METHODS:
Chauffaille, Maria de Lourdes Lopes Ferrari, Kim, Chong Ae, Leone, Cláudio, Okay, Thelma Suely, Sugayama, Sofia Mizuho Miura   +4 more
core   +1 more source

Dynamical investigation of macromolecular hybridization bioassays [PDF]

arXiv, 2002
A novel sensoric technique for the dynamical in situ investigation of a hybridization bio assay is presented, which utilizes a metal bead labeling method. Therein, hybridization results in an increased metal coverage on parts of a substrate, where it takes place.
arxiv  

In Situ Hybridization Pada Kanker Payudara [PDF]

, 2014
Kesulitan yang dijumpai pada penanganan kanker payudara adalah terjadinya kekambuhan atau relaps. Deteksi status HER2 pada pasien merupakan salah satu upaya untuk mendeteksi terjadinya relaps dan juga untuk menentukan jenis terapi yang ada diberikan ...
Diah Witari, Ni Putu
core   +2 more sources

Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue

STAR Protocols, 2023
Summary: Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive.
Rachel Warren, Aftab Shaik, Lauren Teubner, Handeng Lyu, Stijn De Langhe   +4 more
doaj  

Dual-color dual-hapten in situ hybridization (D-DISH) – Comparison with fluorescence in situ hybridization (FISH) for HER2/neu testing in breast cancer

Indian Journal of Pathology and Microbiology, 2020
Context: HER2/neu testing in breast cancer is a mandate due to availability of trastuzumab, a monoclonal antibody targeted against this biomarker.
Pooja K Gajaria, Sonali Tambe, Trupti Pai, Asawari Patil, Sangeeta B Desai, Tanuja M Shet   +5 more
doaj   +1 more source

Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]

, 1992
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Anastasi, Arnoldus, Baldini, Bornkamm, Cremer, Cremer, Cremer, Cremer, Crescenzi, Devilee, Emmerich, Hartl, Heim, Henglein, Hopman, Hopman, Klobeck, Kolluri, Lee, Lengauer, Lengauer, Lichter, Lichter, Lichter, Maniatis, Manolov, Nederlof, Nederlof, Pinkel, Rappold, Tkachuk, Walt, Wiegant, Zech   +33 more
core   +1 more source

Quantification of virus-infected cells using RNA FISH-Flow

STAR Protocols, 2023
Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren, Arturo Barbachano-Guerrero, Devra Huey, Qing Yang, Emma R. Worden-Sapper, Jens H. Kuhn, Sara L. Sawyer   +6 more
doaj