Sorting of chromosomes by magnetic separation [PDF]
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
A Oberteufer+30 more
core +1 more source
Graphene composites: The materials for the future [PDF]
The exotic physical properties of graphene have led to intense research activities on the synthesis and characterization of graphene composites during the last decade. In this article the methods developed for preparation of such materials and the different application areas are reviewed. The composites discussed are of two types, viz; graphene/polymer
arxiv
Digital Light Processing in a Hybrid Atomic Force Microscope: In situ, Nanoscale Characterization of the Printing Process [PDF]
Stereolithography (SLA) and digital light processing (DLP) are powerful additive manufacturing techniques that address a wide range of applications including regenerative medicine, prototyping, and manufacturing. Unfortunately, these printing processes introduce micrometer-scale anisotropic inhomogeneities due to the resin absorptivity, diffusivity ...
arxiv
Electronic Radio-Frequency Refrigerator [PDF]
We demonstrate experimentally that a hybrid single-electron transistor with superconducting leads and a normal-metal island can be refrigerated by an alternating voltage applied to the gate electrode. The simultaneous measurement of the dc current induced by the rf gate through the device at a small bias voltage serves as an in-situ thermometer.
arxiv +1 more source
Contributions of in situ hybridization of sRNA to the study on spatio-temporal gene expression in Hevea brasiliensis [PDF]
Since the dawn of the genomics era, much research has focused on functional studies of genes of interest. In situ hybridization is a method that can be used to precisely localize the expression of a gene in tissues and cells.
Alemanno, Laurence+6 more
core
Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics [PDF]
DNA libraries from sorted human gonosomes were used selectively to stain the X and Y chromosomes in normal and aberrant cultured human cells by chromosomal in situ suppression (CISS-) hybridization.
Cremer, Thomas+5 more
core +1 more source
Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridization
Summary: Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections.
Yunming Wu, C. Ron Yu
doaj
The Evolution of Senses: My Research Journey into the Nervous System of Cnidaria [PDF]
Our understanding of the evolutionary history of animals is improving, but knowledge of the ancient sensory systems that early animals used to interact with their environments is still largely unknown.
Hartley, Molly
core +2 more sources
Prevalence of human papillomavirus DNA in female cervical lesions from Rio de Janeiro, Brazil
A hundred-sixty paraffin-embedded specimens from female cervical lesions were examined for human papillomavirus (HPV) types 6, 11, 16 and 18 infections by non-isotopic in situ hybridization. The data were compared with histologic diagnosis. Eighty-eight (
S. M. B. Cavalcanti+4 more
doaj +1 more source
Understanding early organogenesis using a simplified in situ hybridization protocol in Xenopus [PDF]
Organogenesis is the study of how organs are specified and then acquire their specific shape and functions during development. The Xenopuslaevis embryo is very useful for studying organogenesis because their large size makes them very suitable for ...
Deimling, Steven J.+4 more
core +2 more sources