Results 51 to 60 of about 2,151,594 (302)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

Physalis angulata L. propagation in vitro

open access: yesRevista Ceres, 2019
Physalis angulata L. is a plant with great fruit production potential, mainly in family farms, due to its versatility and valuable products such as jellies and juice concentrates.
João Antônio Ramos de Oliveira   +4 more
doaj   +1 more source

Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System [PDF]

open access: yesInternational Journal of Fertility and Sterility, 2010
Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality ...
Mohsen Forouzanfar   +5 more
doaj   +1 more source

Tracing blastomere fate choices of early embryos in single cell culture [PDF]

open access: yes, 2010
Blastomeres of early vertebrate embryos undergo numerous fate choices for division, motility, pluripotency maintenance and restriction culminating in various cell lineages.
Chang Ming Li   +11 more
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

open access: yesFEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee   +3 more
wiley   +1 more source

Laparoscopic ovum pick up in common marmoset (Callithrix jacchus)

open access: yesTheriogenology Wild
Ovum pick up (OPU) is a crucial assisted reproductive technique for transgenic research of non-human primates, and has commonly been performed via laparotomy in common marmosets (Callithrix jacchus).
Heejong Eom   +5 more
doaj   +1 more source

Excised rootstock roots cultured in vitro

open access: yesPlant Cell Reports, 1998
Root cultures have been established successfully for the Prunus rootstocks Adafuel and Adarcias (Prunus×amygdalopersica), A843 (P. armeniaca), Mariana 2624 (P. cerasifera×munsoniana) and Myrobalan 605AD (P. cerasifera), and for the apple rootstock Jork 9 (Malus×domestica).
Marín, María Luisa   +1 more
openaire   +3 more sources

Culture of human sebocytes in vitro [PDF]

open access: yesDermato-Endocrinology, 2009
Acne and seborrhoea are sebaceous gland-related diseases that are also exclusively human diseases. Therefore, fundamental research on human sebaceous cell function and control requires human models in vitro. The human sebocyte culture model was first introduced in 1989.
Longqing, Xia   +2 more
openaire   +2 more sources

Agent-based computational modeling of wounded epithelial cell monolayers [PDF]

open access: yes, 2004
Computational modeling of biological systems, or ‘in silico biology’ is an emerging tool for understanding structure and order in biological tissues. Computational models of the behavior of epithelial cells in monolayer cell culture have been developed ...
Hill, G.   +4 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

open access: yesFEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura   +2 more
wiley   +1 more source

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