Results 51 to 60 of about 120,147,022 (302)

Structural and biochemical analysis of a B12 superbinder

open access: yesFEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices   +3 more
wiley   +1 more source

Reconstruction of destruction – in vitro reconstitution methods in autophagy research

open access: yes, 2018
International audienceAutophagy is one of the most elaborative membrane remodeling systems in eukaryotic cells. Its major function is to recycle cytoplasmic material by delivering it to lysosomes for degradation.
Wollert, Thomas, Moparthi, Satish Babu
core   +1 more source

Micropropagação do porta-enxerto de macieira 'Seleção 69' tolerante à podridão do colo (Phytophthora cactorum) Micropropagation of 'Seleção 69' apple rootstock tolerant the rotting (Phytophthora cactorum)

open access: yesCiência Rural, 2001
A utilização de porta-enxertos anões, tolerantes a problemas fitossanitários da cultura da macieira, como a podridão do colo (Phytophthora cactorum), pode aumentar o rendimento e reduzir os custos de produção.
Claudete Santa-Catarina   +3 more
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

open access: yesFEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav   +2 more
wiley   +1 more source

Production of Arbuscular Mycorrhizal Fungi using In vitro Root Organ Culture and Phenolic Compounds

open access: yes, 2019
Arbuscular mycorrhizal fungi form obligate symbiotic associations with most of plant families. This limits their in vitro culturing and large-scale production.
Eman Abdullah M. Ali   +3 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

In Vitro Cell. Dev. Biol.-Plant

open access: yes, 2007
Echinacea, better known as purple coneflower, has received a global attention because of its increasing medicinal value. There is enormous potential for the discovery of new medicinal compounds in this species and an immediate need for techniques to ...
Abbasi, Bilal Haider   +3 more
core   +1 more source

Laparoscopic ovum pick up in common marmoset (Callithrix jacchus)

open access: yesTheriogenology Wild
Ovum pick up (OPU) is a crucial assisted reproductive technique for transgenic research of non-human primates, and has commonly been performed via laparotomy in common marmosets (Callithrix jacchus).
Heejong Eom   +5 more
doaj   +1 more source

Physalis angulata L. propagation in vitro

open access: yesRevista Ceres, 2019
Physalis angulata L. is a plant with great fruit production potential, mainly in family farms, due to its versatility and valuable products such as jellies and juice concentrates.
João Antônio Ramos de Oliveira   +4 more
doaj   +1 more source

In vitro fabric culture methods to develop the initial material for triticale breeding in the Volga region

open access: yesЗерновое хозяйство России, 2020
The current paper presents the estimation efficiency results of in vitro fabric culture methods used to develop the initial material for triticale breeding in the Povolzhie.
V. N. Akinina   +3 more
doaj   +1 more source

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