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In vitro maturation

The Obstetrician & Gynaecologist, 2013
Key content In vitro maturation is an alternative to in vitro fertilisation for young women with polycystic ovary syndrome and young ovulatory women. In vitro maturation accomplishes the maturation of immature oocytes in the laboratory, thus minimising or avoiding gonadotrophin stimulation and the associated cost, side‐effects and risks.
Wendy Vitek, Jared C Robins
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In vitro maturation of mammalian spermatozoa

Reviews of Reproduction, 1996
During epididymal transit, mammalian spermatozoa undergo maturation and acquire full fertilizing capacity. The contribution of factors from the epididymal epithelium appears to be essential for this process. Although complete in vitro maturation of epididymal spermatozoa has not been achieved, stages of maturation can be induced under various ...
H D, Moore, M A, Akhondi
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Maturation of the reticulocyte in vitro

Journal of Cell Science, 1984
ABSTRACT The maturation of reticulocytes into erythrocytes was demonstrated in vitro. Reticulocytosis was induced in rats by repeated bleeding or by phenylhydrazine injections. Whole blood samples were then incubated for 2 days at 37 °C.
G, Gronowicz, H, Swift, T L, Steck
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Oocyte maturation in vitro

Current Opinion in Obstetrics and Gynecology, 2002
In contrast to conventional assisted reproduction techniques in which metaphase II oocytes are retrieved for fertilization in vitro, during the in-vitro maturation of oocytes, immature germinal vesicle stage oocytes are retrieved and matured in the laboratory before fertilization and embryo transfer.
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In vitro maturation: a committee opinion

Fertility and Sterility, 2021
The results of in vitro maturation (IVM) investigations suggest the potential for wider clinical application. This document discusses the efficacy of IVM as reported in the published literature to date. This document replaces the document of the same name, last published in 2013.
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Maturation of maize pollen in vitro

Plant Cell Reports, 1992
Maturation of maize pollen was obtained in male reproductive structures cultured in vitro. Immature tassels containing microspores at the mid-uninucleate to late-binucleate stage of development were excised and spikelets, anthers, and/or isolated microspores were cultured on a medium capable of supporting pollen maturation.
D R, Pareddy, J F, Petolino
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Maturation of mouse sperm in vitro

Journal of Experimental Zoology, 1974
AbstractPreincubation of sperm in medium for three hours before mixing with ova increased the number of ova fertilized after one hour, thus indicating that sperm maturation occurs in vitro. The presence of cumulus cells increased the proportion of ova fertilized as observed at two and at three hours after the addition of sperm.
Hoppe, P C, Whitten, W K
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In vitro maturation of horse oocytes

Theriogenology, 1994
Ovaries collected from slaughtered mares of unknown reproductive history were transported to the laboratory, and their oocytes were recovered and cultured in modified TCM 199 supplemented with 20% horse serum and additional granulosa cells. To characterize the ovaries, the size and number of follicles were counted.
H, Alm, H, Torner
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In vitro maturation: a committee opinion

Fertility and Sterility, 2013
The initial results of in vitro maturation suggest the potential for clinical application. However, at this time in vitro maturation should be performed only as an experimental procedure evaluating both efficacy and safety in carefully selected patients.
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Maturation of Schistosome Eggs in vitro

Nature, 1962
EGG-LAYING in vitro by Schistosoma japonicum and S. mansoni in serum media was not described by early investigators1–4, and presumably did not occur. Later it was reported that a few eggs were laid when flukes were kept for up to two months in a glass apparatus with provision for daily change of medium (horse serum)5. A method of maintaining S. mansoni
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