Results 151 to 160 of about 114,907,480 (246)

Implementation of immature oocytes by cryopreservation technique and subsquent in vitro maturation and fertilization post-thawing

, 2011
The aim of this study was to cryopreserve by vitrification by propylene glycol (PROH) and dimethylsulfoxide (DMSO) immature bovine oocytes in straws and to investigate the effects of vitrification on post-thaw oocyte maturation and fertilization. A total of 983 cumulus oocyte complexes were obtained by follicle aspiration from 263 ovaries of cows ...
openaire   +1 more source

Recent Advances and Emerging Applications in Embryo Vitrification for Livestock

Reproduction in Domestic Animals, Volume 61, Issue S3, October 2026.
ABSTRACT Vitrification has progressively supplanted conventional slow freezing as the method of choice for embryo cryopreservation in both human medicine and animal production, particularly for in vitro‐produced embryos and for species or developmental stages highly sensitive to conventional freezing.
Cristina Cuello, Marcos Torregrosa‐Fuentes, Manuela Garcia‐Canovas, Adelina Lopez‐Jara, Inmaculada Parrilla, Maria A. Gil, Emilio A. Martinez   +6 more
wiley   +1 more source

Sperm Recovery and Evaluation in Domestic Species: Challenges and Current Practices

Reproduction in Domestic Animals, Volume 61, Issue S3, October 2026.
ABSTRACT Semen collection is a key step in the development of assisted reproductive technologies (ARTs) in domestic mammals, as the collection method determines the functionality and fertility of the sperm. The artificial vagina or manual collection yields ejaculates similar to those obtained during natural mating, although they require prior training ...
A. J. Soler, O. García‐Álvarez, M. Iniesta‐Cuerda, P. Jiménez‐Rabadán, A. Maroto‐Morales, M. Neila‐Montero, V. Montoro, M. R. Fernández‐Santos, J. J. Garde   +8 more
wiley   +1 more source

Overweight/Obesity and Hyperinsulinemia Impair Oocyte Developmental Competence via Cumulus Cell Dysfunction and Oxidative Stress-Mediated Follicular Microenvironment Remodeling. [PDF]

Int J Mol Sci
Wu T, Zhou Y, Yao G, Ding Y, Hu S, Ding Z.   +5 more
europepmc   +1 more source

Differential Gene Expression of Mesenchymal Stromal Cell and Granulosa Cell Markers in Equine Follicular Aspirates

Reproduction in Domestic Animals, Volume 61, Issue S3, October 2026.
ABSTRACT Equine follicular aspirates obtained during ovum pick‐up contain a heterogeneous mixture of mesenchymal stromal cells (MSC) and granulosa cells (GC). This study evaluates how selective isolation and expansion of MSC decrease GC populations.
María del Prado Soriano‐Campos, Carmen Cristina Muñoz‐García, Marcos Luis‐Calero, Marian del Olmo‐Ortiz, Alejandro Gallardo‐Soler, Beatriz Macías‐García, Lauro González‐Fernández   +6 more
wiley   +1 more source

The use of ex-vivo in-vitro maturation of ovarian tissues oocytes (OTO-IVM) for fertility preservation in gynaecologic cancers. [PDF]

Gynecol Oncol Rep
Goh CSY, Lim CJW, Ng QJ, Lee SH, Chin FHX, Lim YH, Phoon JWL, Sim YJ, Lee JM, Durgahshree M, Hou MCL, Chong YS, Ee TX.   +12 more
europepmc   +1 more source

Functional and Fertility Outcomes of Two Milk‐Based Extenders for Short‐Term Storage of Ram Semen at 15°C

Reproduction in Domestic Animals, Volume 61, Issue S3, October 2026.
ABSTRACT The efficiency of AI in sheep depends partly on maintaining sperm function during storage. This study compared INRA 96 and a skim milk‐based formulation for storing ram semen at 15°C for 6 h, evaluating sperm quality and IVF and field fertility outcomes. INRA 96 showed higher VCL, ALH, and sperm viability after 6 h of storage.
Marta Neila‐Montero, María Iniesta‐Cuerda, Javier Hernández‐Serrano, Eva Díaz‐Hernández, Clara Vidal‐Garcia, Juan Ángel Laborda‐Gomariz, Marta Serrallé, Roberto Gallego, Óscar García‐García, José Julián Garde, Ana Josefa Soler   +10 more
wiley   +1 more source

Enhancing the Oocyte Ultrastructure and Antioxidant Defence System of In Vitro Matured Buffalo Oocytes via Resveratrol Supplementation. [PDF]

Reprod Domest Anim
El-Mahdy AMM, Bakeer L, El-Darawany AA, Shamiah SM, Abdallah EA, Al-Marakby KM, Shukry M, Abdelnour S.   +7 more
europepmc   +1 more source

Influence of Follicle Number and Reproductive Cyclicity on Mesenchymal Stromal Cell Yield Isolated From Equine Ovarian Follicular Aspirates

Reproduction in Domestic Animals, Volume 61, Issue S3, October 2026.
ABSTRACT Follicular aspirates have emerged as an alternative source of mesenchymal stromal cells (MSC) in mares; however, factors influencing successful MSC isolation remain unclear. This study evaluated whether reproductive cyclicity and follicle count affect MSC recovery from ovum pick‐up (OPU)‐derived follicular aspirates.
Carmen C. Muñoz‐García, María del Prado Soriano‐Campos, Marcos Luis‐Calero, Marian del Olmo‐Ortiz, Lauro González‐Fernández, Beatriz Macías‐García   +5 more
wiley   +1 more source

Intrafollicular immature oocyte transfer (IFIOT): technical advances, biological insights and applications in reproductive biotechnologies. [PDF]

Anim Reprod
Sprícigo JFW, Chaves JEV, Dode MAN.
europepmc   +1 more source