Results 161 to 170 of about 25,424 (210)

In vitro spermatogenesis: Why meiotic checkpoints matter

2023
Successful in vitro spermatogenesis would generate functional haploid spermatids, and thus, form the basis for novel approaches to treat patients with impaired spermatogenesis or develop alternative strategies for male fertility preservation. Several culture strategies, including cell cultures using various stem cells and ex vivo cultures of testicular
Lei, Qijing, van Pelt, Ans M. M., Hamer, Geert   +2 more
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Prospects for spermatogenesis in vitro

Theriogenology, 2003
In recent years, extraordinary progress has been made in a broad range of reproductive technologies, including spermatogonial transplantation in the male. However, effective procedures for the complete recapitulation of spermatogenesis in vitro, including meiosis, have remained elusive.
J E, Parks, D R, Lee, S, Huang, M T, Kaproth   +3 more
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Spermatogenesis In Vitro: Searching for In Vivo Correlates

1987
The development of methodology for the study of spermatogenesis in vitro has been a major task that remained elusive (Wolff and Haffen, 1965; Steinberger, 1975). Significant advances have been reported in the culture of meiotic cells of lilaceous plants (Hotta et al., 1966), and spermatogenic cells of Xenopus (Risley, 1983) and Drosophila (Liebrich ...
A L, Kierszenbaum, M, Abdullah, H, Ueda, L L, Tres   +3 more
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Stage-specific approaches promote in vitro induction for spermatogenesis

In Vitro Cellular & Developmental Biology - Animal, 2018
Spermatogenesis in vitro has been demonstrated using spermatogonial stem cells (SSCs) in monolayer culture or testis tissue fragments in agarose-constructed three-dimensional (3-D) conditions. However, the low efficiency of gamete maturation and the lack of a novel induction platform have limited the progress of its use in further research and clinical
Hualin, Yi, Sa, Xiao, Yan, Zhang
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In Vitro Spermatogenesis Using an Organ Culture Technique

2012
Research on in vitro spermatogenesis has a long history and remained to be an unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm from primitive spermatogonia using an organ culture method. The fertility of the sperm or haploid spermatids was demonstrated by microinsemination.
Tetsuhiro, Yokonishi, Takuya, Sato, Kumiko, Katagiri, Takehiko, Ogawa   +3 more
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High glucose levels affect spermatogenesis: an in vitro approach

Reproduction, Fertility and Development, 2016
Besides known factors that may cause male infertility, systemic diseases such as diabetes mellitus may further exacerbate a decline in male fertility. This metabolic disease, clinically characterised by a hyperglycaemic phenotype, has devastating consequences in terms of human health, with reproductive dysfunction being one of the associated clinical ...
Renata S, Tavares, Joana M D, Portela, Maria I, Sousa, Paula C, Mota, João, Ramalho-Santos, Sandra, Amaral   +5 more
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SPERMATOGENESIS IN VITRO: COMPLETION OF MEIOSIS AND EARLY SPERMIOGENESIS

Endocrinology, 1983
In vitro formation of haploid spermatids has not been convincingly demonstrated in mammals. To investigate this problem we selected defined segments of rat seminiferous tubules containing late pachytene and diakinetic primary spermatocytes (Stages XII and XIII of the cycle) for culture in a chemically defined medium.
M, Parvinen, W W, Wright, D M, Phillips, J P, Mather, N A, Musto, C W, Bardin   +5 more
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