Results 41 to 50 of about 25,424 (210)

Time-course microarray transcriptome data of in vitro cultured testes and age-matched in vivo testes

Data in Brief, 2020
In vitro spermatogenesis, which produces fertile spermatozoa, has been successfully performed using an organ culture method from murine tissue. Here, we provide a dataset of time-course microarray transcriptome data of in vitro cultured neonate murine ...
Takeru Abe, Hajime Nishimura, Takuya Sato, Harukazu Suzuki, Takehiko Ogawa, Takahiro Suzuki   +5 more
doaj   +1 more source

Meiotic Chromosome Synapsis and XY-Body Formation In Vitro

Frontiers in Endocrinology, 2021
To achieve spermatogenesis in vitro, one of the most challenging processes to mimic is meiosis. Meiotic problems, like incomplete synapsis of the homologous chromosomes, or impaired homologous recombination, can cause failure of crossover formation and ...
Qijing Lei, Eden Zhang, Ans M. M. van Pelt, Geert Hamer   +3 more
doaj   +1 more source

In Vitro Spermatogenesis; Past, Present, and Future [PDF]

, 2018
DMAB Dissanayake
openalex   +3 more sources

Bridging the Gap: Animal Models in Next-Generation Reproductive Technologies for Male Fertility Preservation

Life, 2023
This review aims to explore advanced reproductive technologies for male fertility preservation, underscoring the essential role that animal models have played in shaping these techniques through historical contexts and into modern applications.
Pedro M. Aponte, Miguel A. Gutierrez-Reinoso, Manuel Garcia-Herreros   +2 more
doaj   +1 more source

Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro

Biology, 2023
The process by which spermatogonial stem cells (SSCs) continuously go through mitosis, meiosis, and differentiation to produce gametes that transmit genetic information is known as spermatogenesis.
Kaiyan Gu, Ya Zhang, Ying Zhong, Yuting Kan, Muhammad Jawad, Lang Gui, Mingchun Ren, Gangchun Xu, Dong Liu, Mingyou Li   +9 more
doaj   +1 more source

CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. [PDF]

PLoS ONE, 2015
Mammalian spermatogenesis is a highly regulated multistage process of sperm generation. It is hard to uncover the real function of a testis specific gene in vitro since the in vitro model is not yet mature.
Minyan Li, Rui Huang, Xue Jiang, Yuxi Chen, Zhen Zhang, Xiya Zhang, Puping Liang, Shaoquan Zhan, Shanbo Cao, Zhou Songyang, Junjiu Huang   +10 more
doaj   +1 more source

Long-Term In Vitro Maintenance of Piglet Testicular Tissue: Effects of Tissue Fragment Size, Preparation Method, and Serum Source

Animals, 2022
Long-term culture of testicular tissue has important applications, including the preservation of fertility potential of prepubertal boys undergoing gonadotoxic cancer treatment.
Fahar Ibtisham, Tat-Chuan Cham, Mohammad Amin Fayaz, Ali Honaramooz   +3 more
doaj   +1 more source

Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta)

Asian Journal of Andrology, 2015
In vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-dimensional (2D) culture systems; however, such cultures have not led to the development of complete spermatogenesis.
Mahmoud Huleihel, Seyedmehdi Nourashrafeddin, Tony M Plant   +2 more
doaj   +1 more source

Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Frontiers in Physiology, 2021
Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule ...
Guillaume Richer, Robin M. Hobbs, Katherine L. Loveland, Katherine L. Loveland, Ellen Goossens, Yoni Baert   +5 more
doaj   +1 more source

Human immature testicular tissue organ culture: a step towards fertility preservation and restoration

Frontiers in Endocrinology, 2023
BackgroundCryopreservation of immature testicular tissue (ITT) is currently the only option to preserve fertility of prepubertal patients. Autologous transplantation of ITT may not be safe or appropriate for all patients. Therefore, methods to mature ITT
Nagham Younis, Nagham Younis, Andre L. Caldeira-Brant, Tianjiao Chu, Shtaywy Abdalla, Kyle E. Orwig   +5 more
doaj   +1 more source