Results 31 to 40 of about 27,005 (105)

Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Frontiers in Physiology, 2021
Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule ...
Guillaume Richer, Robin M. Hobbs, Katherine L. Loveland, Katherine L. Loveland, Ellen Goossens, Yoni Baert   +5 more
doaj   +1 more source

A Review on Application of Three Dimensional Culture and Testicular Scaffolds to Induction of in-Vitro Spermatogenesis

Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd, 2020
Introduction: Induction of in vitro spermatogenesis can be useful for infertility treatment in azoospermic patients and those undergoing chemotherapy. Different culture systems have been used to achieve this goal. This review study was performed with the
Nasrin Majidi Gharenaz, Mansoureh Movahedin, Samiyeh Majidi, Zohreh Mazaheri   +3 more
doaj  

Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro

Biology, 2022
Opsariichthys bidens belongs to the family Cyprinidae and is a small freshwater economic fish widely distributed in China. In recent years, the natural resources of O.
Xiao Chen, Yuting Kan, Ying Zhong, Muhammad Jawad, Wenbo Wei, Kaiyan Gu, Lang Gui, Mingyou Li   +7 more
doaj   +1 more source

Reverse transcriptase inhibitors enable the generation of fertile spermatids from fetal mouse testes in vitro

Communications Biology
Organ culture systems enabling in vitro spermatogenesis from neonatal mouse testes exist, but differentiation from fetal testes shortly after sex determination remains unsuccessful.
Mayuka Nishida, Yukina Ono-Sunagare, Sayuri Kato, Yu Ishikawa-Yamauchi, Takafumi Matsumura, Mitsuru Komeya, Shogo Matoba, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura, Takehiko Ogawa, Takuya Sato   +11 more
doaj   +1 more source

Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining

Journal of Animal Reproduction and Biotechnology, 2015
In the study for a differentiation and development of spermatogonial cells, the researchers should commonly require a simple, fast and reasonable method that could evaluate the developmental stage of male germ cells without any damage and also ...
Kyoung Guk Kim, Young Sik Park
doaj   +1 more source

Sex chromosome aneuploidies and fertility: 47,XXY, 47,XYY, 47,XXX and 45,X/47,XXX

Endocrine Connections, 2023
The overall incidence of sex chromosome aneuploidies is approximately 1 per 500 live-born infants, but far more common at conception. I shall review the fertility aspects of the sex chromosome trisomies, XXY, XYY, and XXX, with special reference to the ...
Alan D Rogol
doaj   +1 more source

Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids

Advanced Science
Testicular organoids (TOs) offer an opportunity to preserve fertility, but current TO protocols are limited by suboptimal maintenance of the organoid structure, meiotic defects, and incomplete in vitro spermatogenesis (IVS). Here, a strategy is developed
Jiachen Sun, Lifa Zhu, Yutong Li, Jinyan Tang, Tao Zhang, Hengjia Zhang, Wanchu Wang, Lufan Li, Shuhui Bian, Xin Wu   +9 more
doaj   +1 more source

A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular Niche

Advanced Science
Childhood cancer treatments often impair male fertility due to gonadotoxic effects. While in vitro culture of prepubertal testis tissue offers a potential solution to preserve fertility, producing sufficient sperm remains a major barrier. To address this,
Yu Xia, Xiaoxuan Zhang, Bohang Zhang, Minhui Lu, Qing Cheng, Li Liu, Lifang Shi, Yiqiang Cui, Dingdong Chen, Laihua Li, Xuejiang Guo, Jingtao Guo, Jiahao Sha, Yuanjin Zhao, Yan Yuan   +14 more
doaj   +1 more source

Induction of autophagy via the ROS-dependent AMPK-mTOR pathway protects copper-induced spermatogenesis disorder

Redox Biology, 2022
Copper (Cu) is a necessary micronutrient at lower concentration, while excessive Cu exposure or Cu homeostasis disorders can lead to toxicity. The mechanism of male reproductive toxicity induced by Cu is still unknown.
Hongrui Guo, Yujuan Ouyang, Heng Yin, Hengmin Cui, Huidan Deng, Huan Liu, Zhijie Jian, Jing Fang, Zhicai Zuo, Xun Wang, Ling Zhao, Yanqiu Zhu, Yi Geng, Ping Ouyang   +13 more
doaj   +1 more source

Human immature testicular tissue organ culture: a step towards fertility preservation and restoration

Frontiers in Endocrinology, 2023
BackgroundCryopreservation of immature testicular tissue (ITT) is currently the only option to preserve fertility of prepubertal patients. Autologous transplantation of ITT may not be safe or appropriate for all patients. Therefore, methods to mature ITT
Nagham Younis, Nagham Younis, Andre L. Caldeira-Brant, Tianjiao Chu, Shtaywy Abdalla, Kyle E. Orwig   +5 more
doaj   +1 more source