Results 41 to 50 of about 27,005 (105)

Microfluidic and Static Organotypic Culture Systems to Support Ex Vivo Spermatogenesis From Prepubertal Porcine Testicular Tissue: A Comparative Study

Frontiers in Physiology, 2022
Background:In vitro maturation of immature testicular tissue (ITT) cryopreserved for fertility preservation is a promising fertility restoration strategy. Organotypic tissue culture proved successful in mice, leading to live births.
Marc Kanbar, Marc Kanbar, Francesca de Michele, Francesca de Michele, Jonathan Poels, Jonathan Poels, Stéphanie Van Loo, Maria Grazia Giudice, Maria Grazia Giudice, Tristan Gilet, Christine Wyns, Christine Wyns   +11 more
doaj   +1 more source

In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors

Stem Cell Research & Therapy
Background Culture medium enriched with Knockout serum replacement (KSR) can produce in vitro mouse sperm, but it is inefficient, strain-specific and contains bovine products, which limits its use in the human clinic.
Seyyed Amir Moradian, Mansoureh Movahedin   +1 more
doaj   +1 more source

Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro

Aquaculture Reports
Southern catfish (Silurus meridionalis) is widely distributed in the Yangtze River and its tributaries and is valued for its rapid growth, nutritional content, pleasant taste, absence of intermuscular bone, and disease resistance.
Jianeng Li, Changle Zhao, Lei Liu, Shuqing Zheng, Deshou Wang, Changwei Shao, Jing Wei   +6 more
doaj   +1 more source

Maturation-associated changes in Sertoli cells following in vitro culture of frozen-thawed prepubertal mouse testicular tissue

Frontiers in Reproductive Health
To preserve the fertility of prepubertal boys with cancer, testicular tissue can be cryopreserved in the hope of subsequently inducing their maturation after transplantation or in vitro culture for natural conception or medically assisted reproduction ...
Laura Moutard, Laura Moutard, Marion Dufour, Nathalie Rives, Nathalie Rives, Nathalie Rives, Magali Basille-Dugay, Magali Basille-Dugay, Eva Chemin, Eva Chemin, Valentin Rousseau, Valentin Rousseau, Aurélie Feraille, Aurélie Feraille, Aurélie Feraille, Christine Rondanino, Christine Rondanino, Ludovic Dumont, Ludovic Dumont   +18 more
doaj   +1 more source

Distinct roles of melatonin receptors in spermatogenesis in vitro in four-eyed sleeper (Bostrychus sinensis)

Aquaculture Reports
Melatonin is a master neuroendocrine hormone in regulation of circadian rhythm and seasonal breeding behaviors in animal kingdom. Recently, we used the scaffold material to establish the three-dimension (3D) culture method, by which we found that ...
Jiajun Yao, Haifeng Li, Han Yang, Meisheng Yi, Wei Liu   +4 more
doaj   +1 more source

Biology and manipulation technologies of male germline stem cells in mammals

Reproductive Medicine and Biology, 2018
Background Spermatogonial stem cells (SSCs) are the origin of sperm and defined by their functions of “colonization in the testis” and “spermatogenesis”.
Seiji Takashima
doaj   +1 more source

Ubiquitous Flame-Retardant Toxicants Impair Spermatogenesis in a Human Stem Cell Model

iScience, 2018
Summary: Sperm counts have rapidly declined in Western males over the past four decades. This rapid decline remains largely unexplained, but exposure to environmental toxicants provides one potential explanation for this decline.
Alyse N. Steves, Joshua M. Bradner, Kristen L. Fowler, Danielle Clarkson-Townsend, Brittany J. Gill, Adam C. Turry, W. Michael Caudle, Gary W. Miller, Anthony W.S. Chan, Charles A. Easley, IV   +9 more
doaj   +1 more source

Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis. [PDF]

Stem Cell Res Ther, 2023
Salem M, Khadivi F, Javanbakht P, Mojaverrostami S, Abbasi M, Feizollahi N, Abbasi Y, Heidarian E, Rezaei Yazdi F.   +8 more
europepmc   +1 more source

In vitro spermatogenesis in isolated seminiferous tubules of immature mice. [PDF]

PLoS One, 2023
Feng X, Matsumura T, Yamashita Y, Sato T, Hashimoto K, Odaka H, Makino Y, Okada Y, Nakamura H, Kimura H, Fujii T, Ogawa T.   +11 more
europepmc   +1 more source

The dynamic expression of YAP is essential for the development of male germ cells derived from human embryonic stem cells

Scientific Reports
YAP plays a vital role in controlling growth and differentiation in various cell lineages. Although the expression of YAP in mice testicular and spermatogenic cells suggests its role in mammalian spermatogenesis, the role of YAP in the development of ...
Sujittra Khampang, Chanchao Lorthongpanich, Chuti Laowtammathron, Phatchanat Klaihmon, Sukanya Meesa, Wichuda Suksomboon, Nittaya Jiamvoraphong, Pakpoom Kheolamai, Sudjit Luanpitpong, Charles A. Easley, Eisa Mahyari, Surapol Issaragrisil   +11 more
doaj   +1 more source