Results 41 to 50 of about 114,054,514 (226)

Effect of FSH on testicular morphology and spermatogenesis in gonadotrophin-deficient hypogonadal mice lacking androgen receptors [PDF]

, 2010
Follicle stimulating hormone (FSH) and androgen act to stimulate and maintain spermatogenesis. FSH acts directly on the Sertoli cells to stimulate germ cell number and acts indirectly to increase androgen production by the Leydig cells.
Abel, M.H., Verhoeven, G., De Gendt, K., Monteiro, A., O'Shaughnessy, P.J.   +4 more
core   +1 more source

Meiotic Chromosome Synapsis and XY-Body Formation In Vitro

Frontiers in Endocrinology, 2021
To achieve spermatogenesis in vitro, one of the most challenging processes to mimic is meiosis. Meiotic problems, like incomplete synapsis of the homologous chromosomes, or impaired homologous recombination, can cause failure of crossover formation and ...
Qijing Lei, Eden Zhang, Ans M. M. van Pelt, Geert Hamer   +3 more
doaj   +1 more source

X chromosome inactivation during Drosophila spermatogenesis [PDF]

, 2007
Genes with male- and testis-enriched expression are under-represented on the Drosophila melanogaster X chromosome. There is also an excess of retrotransposed genes, many of which are expressed in testis, that have “escaped” the X chromosome and moved to ...
Baines, J., Hense, Winfried, Parsch, John, John Parsch, Baines, John F., Parsch John, Hense Winfried, Baines John F, Hense, W., John F Baines, Winfried Hense, Parsch, J.   +11 more
core   +2 more sources

Bridging the Gap: Animal Models in Next-Generation Reproductive Technologies for Male Fertility Preservation

Life, 2023
This review aims to explore advanced reproductive technologies for male fertility preservation, underscoring the essential role that animal models have played in shaping these techniques through historical contexts and into modern applications.
Pedro M. Aponte, Miguel A. Gutierrez-Reinoso, Manuel Garcia-Herreros   +2 more
doaj   +1 more source

Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro

Biology, 2023
The process by which spermatogonial stem cells (SSCs) continuously go through mitosis, meiosis, and differentiation to produce gametes that transmit genetic information is known as spermatogenesis.
Kaiyan Gu, Ya Zhang, Ying Zhong, Yuting Kan, Muhammad Jawad, Lang Gui, Mingchun Ren, Gangchun Xu, Dong Liu, Mingyou Li   +9 more
doaj   +1 more source

In vitro mouse spermatogenesis with an organ culture method in chemically defined medium. [PDF]

PLoS ONE, 2018
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or ...
Hiroyuki Sanjo, Mitsuru Komeya, Takuya Sato, Takeru Abe, Kumiko Katagiri, Hiroyuki Yamanaka, Yoko Ino, Noriaki Arakawa, Hisashi Hirano, Tatsuma Yao, Yuta Asayama, Akio Matsuhisa, Masahiro Yao, Takehiko Ogawa   +13 more
doaj   +1 more source

CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. [PDF]

PLoS ONE, 2015
Mammalian spermatogenesis is a highly regulated multistage process of sperm generation. It is hard to uncover the real function of a testis specific gene in vitro since the in vitro model is not yet mature.
Minyan Li, Rui Huang, Xue Jiang, Yuxi Chen, Zhen Zhang, Xiya Zhang, Puping Liang, Shaoquan Zhan, Shanbo Cao, Zhou Songyang, Junjiu Huang   +10 more
doaj   +1 more source

Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta)

Asian Journal of Andrology, 2015
In vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-dimensional (2D) culture systems; however, such cultures have not led to the development of complete spermatogenesis.
Mahmoud Huleihel, Seyedmehdi Nourashrafeddin, Tony M Plant   +2 more
doaj   +1 more source

Long-Term In Vitro Maintenance of Piglet Testicular Tissue: Effects of Tissue Fragment Size, Preparation Method, and Serum Source

Animals, 2022
Long-term culture of testicular tissue has important applications, including the preservation of fertility potential of prepubertal boys undergoing gonadotoxic cancer treatment.
Fahar Ibtisham, Tat-Chuan Cham, Mohammad Amin Fayaz, Ali Honaramooz   +3 more
doaj   +1 more source

Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Frontiers in Physiology, 2021
Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule ...
Guillaume Richer, Robin M. Hobbs, Katherine L. Loveland, Katherine L. Loveland, Ellen Goossens, Yoni Baert   +5 more
doaj   +1 more source