Results 21 to 30 of about 27,005 (105)

Throughout in vitro first spermatogenic wave: Next-generation sequencing gene expression patterns of fresh and cryopreserved prepubertal mice testicular tissue explants

Frontiers in Endocrinology, 2023
IntroductionSuitable cryopreservation procedures of pre-pubertal testicular tissue associated with efficient culture conditions are crucial in the fields of fertility preservation and restoration.
Ludovic Dumont, Ludovic Dumont, Hélène Lopez Maestre, Hélène Lopez Maestre, Frédéric Chalmel, Louise Huber, Louise Huber, Aurélie Rives-Feraille, Aurélie Rives-Feraille, Laura Moutard, Laura Moutard, Frédérique Bateux, Frédérique Bateux, Christine Rondanino, Christine Rondanino, Nathalie Rives, Nathalie Rives, Nathalie Rives   +17 more
doaj   +1 more source

Advanced bioengineering of male germ stem cells to preserve fertility

Journal of Tissue Engineering, 2021
In modern life, several factors such as genetics, exposure to toxins, and aging have resulted in significant levels of male infertility, estimated to be approximately 18% worldwide.
Hossein Eyni, Sadegh Ghorbani, Hojjatollah Nazari, Marziyeh Hajialyani, Sajad Razavi Bazaz, Mahdi Mohaqiq, Majid Ebrahimi Warkiani, Duncan S Sutherland   +7 more
doaj   +1 more source

Assessment of the toxicity effects of nicotine on sperm and IVF and the potential protective role of silymarin—an experimental study in mice

Middle East Fertility Society Journal, 2020
Background Male infertility is usually caused via the inability to produce adequate quantities of healthy and active sperms. Nicotine (NIC) is an alkaloid organic compound, predominantly found in the leaves of the tobacco plant.
Mohammad Rahimi-Madiseh, Mohsen Mohammadi, Amin Hassanvand, Reza Ahmadi, Maasume Shahmohammadi, Ayoob Rostamzadeh   +5 more
doaj   +1 more source

Meiotic Chromosome Synapsis and XY-Body Formation In Vitro

Frontiers in Endocrinology, 2021
To achieve spermatogenesis in vitro, one of the most challenging processes to mimic is meiosis. Meiotic problems, like incomplete synapsis of the homologous chromosomes, or impaired homologous recombination, can cause failure of crossover formation and ...
Qijing Lei, Eden Zhang, Ans M. M. van Pelt, Geert Hamer   +3 more
doaj   +1 more source

Bridging the Gap: Animal Models in Next-Generation Reproductive Technologies for Male Fertility Preservation

Life, 2023
This review aims to explore advanced reproductive technologies for male fertility preservation, underscoring the essential role that animal models have played in shaping these techniques through historical contexts and into modern applications.
Pedro M. Aponte, Miguel A. Gutierrez-Reinoso, Manuel Garcia-Herreros   +2 more
doaj   +1 more source

Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro

Biology, 2023
The process by which spermatogonial stem cells (SSCs) continuously go through mitosis, meiosis, and differentiation to produce gametes that transmit genetic information is known as spermatogenesis.
Kaiyan Gu, Ya Zhang, Ying Zhong, Yuting Kan, Muhammad Jawad, Lang Gui, Mingchun Ren, Gangchun Xu, Dong Liu, Mingyou Li   +9 more
doaj   +1 more source

In vitro mouse spermatogenesis with an organ culture method in chemically defined medium. [PDF]

PLoS ONE, 2018
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or ...
Hiroyuki Sanjo, Mitsuru Komeya, Takuya Sato, Takeru Abe, Kumiko Katagiri, Hiroyuki Yamanaka, Yoko Ino, Noriaki Arakawa, Hisashi Hirano, Tatsuma Yao, Yuta Asayama, Akio Matsuhisa, Masahiro Yao, Takehiko Ogawa   +13 more
doaj   +1 more source

Long-Term In Vitro Maintenance of Piglet Testicular Tissue: Effects of Tissue Fragment Size, Preparation Method, and Serum Source

Animals, 2022
Long-term culture of testicular tissue has important applications, including the preservation of fertility potential of prepubertal boys undergoing gonadotoxic cancer treatment.
Fahar Ibtisham, Tat-Chuan Cham, Mohammad Amin Fayaz, Ali Honaramooz   +3 more
doaj   +1 more source

CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. [PDF]

PLoS ONE, 2015
Mammalian spermatogenesis is a highly regulated multistage process of sperm generation. It is hard to uncover the real function of a testis specific gene in vitro since the in vitro model is not yet mature.
Minyan Li, Rui Huang, Xue Jiang, Yuxi Chen, Zhen Zhang, Xiya Zhang, Puping Liang, Shaoquan Zhan, Shanbo Cao, Zhou Songyang, Junjiu Huang   +10 more
doaj   +1 more source

Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta)

Asian Journal of Andrology, 2015
In vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-dimensional (2D) culture systems; however, such cultures have not led to the development of complete spermatogenesis.
Mahmoud Huleihel, Seyedmehdi Nourashrafeddin, Tony M Plant   +2 more
doaj   +1 more source