In vitro spermatogenesis: A century‐long research journey, still half way around [PDF]
Reproductive Medicine and Biology, 2018 Background Spermatogenesis is one of the most complicated cellular differentiation processes in a body. Researchers struggled to find and develop a micro‐environmental condition that can support the process in vitro.
Mitsuru Komeya +2 more
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In vitro spermatogenesis: In search of fully defined conditions [PDF]
Frontiers in Cell and Developmental Biology, 2023 A complete reconstruction of spermatogenesis in vitro under fully defined conditions still has not been achieved. However, many techniques have been proposed to get closer to that aim. Here we review the current progress in the field.
A. Yu. Kulibin, E. A. Malolina
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Development of the membrane ceiling method for in vitro spermatogenesis [PDF]
Scientific ReportsSpermatogenesis is one of the most complex processes of cell differentiation and its failure is a major cause of male infertility. Therefore, a proper model that recapitulates spermatogenesis in vitro has been long sought out for basic and clinical ...
Maki Kamoshita +11 more
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Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control [PDF]
The Journal of Reproduction and Development, 2023 Incorporation of bovine serum-derived albumin formulation (AlbuMAX) into a basic culture medium, MEMα, enables the completion of in vitro spermatogenesis through testicular tissue culture in mice.
Takehiko OGAWA +6 more
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Generation of rat offspring using spermatids produced through in vitro spermatogenesis [PDF]
Scientific Reports, 2023 An in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture method has generally not been effective in other animal species, we recently succeeded in improving ...
Takafumi Matsumura +11 more
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Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy [PDF]
Scientific Reports, 2022 The assessment of the impact of chemotherapies on in vitro spermatogenesis in experimental models is required before considering the application of this fertility restoration strategy to prepubertal boys who received these treatments before testicular ...
Marion Delessard +7 more
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A pumpless monolayer microfluidic device based on mesenchymal stem cell-conditioned medium promotes neonatal mouse in vitro spermatogenesis [PDF]
Stem Cell Research & Therapy, 2023 Background Childhood cancer treatment-induced gonadotoxicity causes permanent infertility/sub-infertility in nearly half of males. The current clinical and experimental approaches are limited to cryopreservation of prepubertal testicular strips and in ...
Selin Önen +6 more
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In Vitro Spermatogenesis in Explanted Adult Mouse Testis Tissues. [PDF]
PLoS ONE, 2015 Research on in vitro spermatogenesis is important for elucidating the spermatogenic mechanism. We previously developed an organ culture method which can support spermatogenesis from spermatogonial stem cells up to sperm formation using immature mouse ...
Takuya Sato +5 more
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Recent Developments in In Vitro Spermatogenesis and Future Directions
Reproductive Medicine, 2023 Recent developments in stem cell technologies have made significant advancements in the field of in vitro gametogenesis. In vitro gametogenesis (IVG) is a promising technology where functional gametes (sperm or egg cells) can be generated from stem cells.
In Ki Cho, Charles A. Easley
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In vitro spermatogenesis in isolated seminiferous tubules of immature mice. [PDF]
PLoS One, 2023 Mouse spermatogenesis, from spermatogonial stem cell proliferation to sperm formation, can be reproduced in vitro by culturing testis tissue masses of neonatal mice. However, it remains to be determined whether this method is also applicable when testis tissues are further divided into tiny fragments, such as segments of the seminiferous tubule (ST), a
Feng X +11 more
europepmc +5 more sources