In vitro spermatogenesis: A century‐long research journey, still half way around [PDF]
Reproductive Medicine and Biology, 2018 Background Spermatogenesis is one of the most complicated cellular differentiation processes in a body. Researchers struggled to find and develop a micro‐environmental condition that can support the process in vitro.
Mitsuru Komeya +2 more
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Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control [PDF]
The Journal of Reproduction and Development, 2023 Incorporation of bovine serum-derived albumin formulation (AlbuMAX) into a basic culture medium, MEMα, enables the completion of in vitro spermatogenesis through testicular tissue culture in mice.
Takehiko OGAWA +6 more
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Generation of rat offspring using spermatids produced through in vitro spermatogenesis [PDF]
Scientific Reports, 2023 An in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture method has generally not been effective in other animal species, we recently succeeded in improving ...
Takafumi Matsumura +11 more
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Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy [PDF]
Scientific Reports, 2022 The assessment of the impact of chemotherapies on in vitro spermatogenesis in experimental models is required before considering the application of this fertility restoration strategy to prepubertal boys who received these treatments before testicular ...
Marion Delessard +7 more
doaj +2 more sources
A pumpless monolayer microfluidic device based on mesenchymal stem cell-conditioned medium promotes neonatal mouse in vitro spermatogenesis [PDF]
Stem Cell Research & Therapy, 2023 Background Childhood cancer treatment-induced gonadotoxicity causes permanent infertility/sub-infertility in nearly half of males. The current clinical and experimental approaches are limited to cryopreservation of prepubertal testicular strips and in ...
Selin Önen +6 more
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Recent Developments in In Vitro Spermatogenesis and Future Directions
Reproductive Medicine, 2023 Recent developments in stem cell technologies have made significant advancements in the field of in vitro gametogenesis. In vitro gametogenesis (IVG) is a promising technology where functional gametes (sperm or egg cells) can be generated from stem cells.
In Ki Cho, Charles A. Easley
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In vitro spermatogenesis: In search of fully defined conditions [PDF]
Frontiers in Cell and Developmental Biology, 2023 A complete reconstruction of spermatogenesis in vitro under fully defined conditions still has not been achieved. However, many techniques have been proposed to get closer to that aim. Here we review the current progress in the field.
A. Yu. Kulibin, E. A. Malolina
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In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues [PDF]
Reproductive Medicine and Biology, 2019 Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at the interphase between culture medium and air.
Mitsuru Komeya +8 more
doaj +2 more sources
Combination of knockout serum replacement and plasma rich in growth factors does not support in vitro spermatogenesis in mice [PDF]
Scientific ReportsCancer treatments can lead to infertility, particularly in prepubertal boys who cannot preserve sperm before therapy. In vitro spermatogenesis offers a promising strategy for fertility preservation in this population by enabling the development of sperm ...
Seyyed Amir Moradian +3 more
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In vivo and in vitro spermatogenesis in prepubertal mouse testes exposed to low gonadotoxic doses of cytarabine or Daunorubicin [PDF]
Scientific ReportsThe development of experimental models treated by chemotherapy is needed for elucidating the side effects of cancer treatments administered prior to puberty on male gonad function and the feasibility of restoring fertility from exposed testicular tissues.
Marion Delessard +7 more
doaj +2 more sources