Results 11 to 20 of about 114,054,514 (226)
Models of in vitro spermatogenesis [PDF]
Spermatogenesis, 2012 Understanding the mechanisms that lead to the differentiation of male germ cells from their spermatogonial stem cells through meiosis to give rise to mature haploid spermatozoa has been a major quest for many decades. Unlike most other cell types this differentiation process is more or less completely dependent upon the cells being located within the ...
Hunter, D. +3 more
openaire +4 more sources
Spermatogenesis in vitro: myth or reality? [PDF]
, 2022 In vitro spermatogenesis has been investigated using embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) or spermatogonial stem cells (SSCs) by many laboratories worldwide. However, for human fertility preservation, patient-specific ESCs are not available and the safety of using these cells to generate gametes that transfer their genome
Lei, Qijing, Hamer, G.
core +11 more sources
Discovering in vitro spermatogenesis stimulating factors [PDF]
Cell Death & Disease, 2015 Spermatozoan development encompasses pre-meiotic, meiotic and post-meiotic cellular processes adapted to genetically diversify and then vertically transmit haploid genomes via fertilization. Defined culture systems supporting spermatogonial stem cell differentiation through meiosis will provide long-sought-after experimental platforms to study ...
Chaudhary, J, Hamra, F K
openaire +3 more sources
In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues [PDF]
Reproductive Medicine and Biology, 2019 Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at the interphase between culture medium and air.
Mitsuru Komeya +8 more
doaj +2 more sources
Combination of knockout serum replacement and plasma rich in growth factors does not support in vitro spermatogenesis in mice [PDF]
Scientific ReportsCancer treatments can lead to infertility, particularly in prepubertal boys who cannot preserve sperm before therapy. In vitro spermatogenesis offers a promising strategy for fertility preservation in this population by enabling the development of sperm ...
Seyyed Amir Moradian +3 more
doaj +2 more sources
Progress and future prospect of in vitro spermatogenesis [PDF]
Oncotarget, 2017 Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40-50% is due to "male factor" infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment.
Ibtisham, Fahar +7 more
openaire +4 more sources
In vivo and in vitro spermatogenesis in prepubertal mouse testes exposed to low gonadotoxic doses of cytarabine or Daunorubicin [PDF]
Scientific ReportsThe development of experimental models treated by chemotherapy is needed for elucidating the side effects of cancer treatments administered prior to puberty on male gonad function and the feasibility of restoring fertility from exposed testicular tissues.
Marion Delessard +7 more
doaj +2 more sources
Spermatogenesis, 2012 Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro, but no efficient system has been established yet that covers the entire process of mammalian spermatogenesis in a culture dish permanently. In this review, we report on the requirements of spermatogenesis and the current state of different culture methods using ...
Reuter, Karin +3 more
openaire +3 more sources
Recent Progress of Induced Spermatogenesis In Vitro
International Journal of Molecular SciencesSperm, a crucial gamete for reproduction in sexual reproduction, is generated through the proliferation, differentiation, and morphological transformations of spermatogonial stem cells within the specialized microenvironment of the testes. Replicating this environment artificially presents challenges. However, interdisciplinary advancements in physics,
Jiang Wu +2 more
exaly +3 more sources
Maturation-associated changes in Sertoli cells following in vitro culture of frozen-thawed prepubertal mouse testicular tissue [PDF]
Frontiers in Reproductive HealthTo preserve the fertility of prepubertal boys with cancer, testicular tissue can be cryopreserved in the hope of subsequently inducing their maturation after transplantation or in vitro culture for natural conception or medically assisted reproduction ...
Laura Moutard +18 more
doaj +2 more sources