Results 11 to 20 of about 51,909 (235)

In vitro spermatogenesis [PDF]

Spermatogenesis, 2012
The generation of functional sperm in vitro has been a goal for almost a century. Until recently, researchers have only succeeded in reproducing the early steps of spermatogenesis. This is not surprising given that spermatogenesis is a complicated process that requires the coordinated efforts of germ cells and several somatic cells within the tubular ...
Miles F Wilkinson
exaly   +3 more sources

Cryopreservation of testis tissues and in vitro spermatogenesis. [PDF]

Reprod Med Biol, 2016
AbstractCancer treatments, either chemo‐ or radiotherapy, may cause severe damage to gonads which could lead to the infertility of patients. In post‐pubertal male patients, semen cryopreservation is recommended to preserve the potential to have their own biological children in the future; however, it is not applicable to prepubertals.
Yokonishi T, Ogawa T.
europepmc   +4 more sources

Reverse transcriptase inhibitors enable the generation of fertile spermatids from fetal mouse testes in vitro [PDF]

Communications Biology
Organ culture systems enabling in vitro spermatogenesis from neonatal mouse testes exist, but differentiation from fetal testes shortly after sex determination remains unsuccessful.
Mayuka Nishida, Yukina Ono-Sunagare, Sayuri Kato, Yu Ishikawa-Yamauchi, Takafumi Matsumura, Mitsuru Komeya, Shogo Matoba, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura, Takehiko Ogawa, Takuya Sato   +11 more
doaj   +2 more sources

Discovering in vitro spermatogenesis stimulating factors. [PDF]

Cell Death Dis, 2015
Spermatozoan development encompasses pre-meiotic, meiotic and post-meiotic cellular processes adapted to genetically diversify and then vertically transmit haploid genomes via fertilization. Defined culture systems supporting spermatogonial stem cell differentiation through meiosis will provide long-sought-after experimental platforms to study ...
Chaudhary J, Hamra FK.
europepmc   +4 more sources

In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues [PDF]

Reproductive Medicine and Biology, 2019
Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at the interphase between culture medium and air.
Mitsuru Komeya, Hiroyuki Yamanaka, Hiroyuki Sanjo, Masahiro Yao, Hiroko Nakamura, Hiroshi Kimura, Teruo Fujii, Takuya Sato, Takehiko Ogawa   +8 more
doaj   +2 more sources

Combination of knockout serum replacement and plasma rich in growth factors does not support in vitro spermatogenesis in mice [PDF]

Scientific Reports
Cancer treatments can lead to infertility, particularly in prepubertal boys who cannot preserve sperm before therapy. In vitro spermatogenesis offers a promising strategy for fertility preservation in this population by enabling the development of sperm ...
Seyyed Amir Moradian, Sajed Khaledi, Zahra Amirkhani, Mansoureh Movahedin   +3 more
doaj   +2 more sources

Progress and future prospect of in vitro spermatogenesis. [PDF]

Oncotarget, 2017
Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40-50% is due to "male factor" infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment.
Ibtisham F, Wu J, Xiao M, An L, Banker Z, Nawab A, Zhao Y, Li G.   +7 more
europepmc   +4 more sources

Fact or fiction: In vitro spermatogenesis. [PDF]

Spermatogenesis, 2012
Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro, but no efficient system has been established yet that covers the entire process of mammalian spermatogenesis in a culture dish permanently. In this review, we report on the requirements of spermatogenesis and the current state of different culture methods using ...
Reuter K, Schlatt S, Ehmcke J, Wistuba J.   +3 more
europepmc   +4 more sources

In vivo and in vitro spermatogenesis in prepubertal mouse testes exposed to low gonadotoxic doses of cytarabine or Daunorubicin [PDF]

Scientific Reports
The development of experimental models treated by chemotherapy is needed for elucidating the side effects of cancer treatments administered prior to puberty on male gonad function and the feasibility of restoring fertility from exposed testicular tissues.
Marion Delessard, Laura Moutard, Coline Charnay, Nathalie Rives, Ludovic Dumont, Magali Basille-Dugay, Aurélie Feraille, Christine Rondanino   +7 more
doaj   +2 more sources

Advances in human In vitro spermatogenesis: A review

Molecular Aspects of Medicine
Recent advances surrounding in vitro spermatogenesis (IVS) have shown potential in creating a new paradigm of regenerative medicine in the future of fertility treatments for males experiencing non-obstructive azoospermia (NOA). Male infertility is a common condition affecting approximately 15% of couples, with azoospermia being present in 15% of ...
Anna-Lisa V Nguyen, Ryan Flannigan
exaly   +3 more sources