Results 11 to 20 of about 25,802 (201)

Development of the membrane ceiling method for in vitro spermatogenesis [PDF]

Scientific Reports
Spermatogenesis is one of the most complex processes of cell differentiation and its failure is a major cause of male infertility. Therefore, a proper model that recapitulates spermatogenesis in vitro has been long sought out for basic and clinical ...
Maki Kamoshita, Hiroki Shirai, Hiroko Nakamura, Tetsuya Kishimoto, Yuki Hatanaka, Daisuke Mashiko, Katsuhiro Esashika, Jingjing Yang, Satoshi Yamasaki, Takehiko Ogawa, Hiroshi Kimura, Masahito Ikawa   +11 more
doaj   +4 more sources

Combination of knockout serum replacement and plasma rich in growth factors does not support in vitro spermatogenesis in mice [PDF]

Scientific Reports
Cancer treatments can lead to infertility, particularly in prepubertal boys who cannot preserve sperm before therapy. In vitro spermatogenesis offers a promising strategy for fertility preservation in this population by enabling the development of sperm ...
Seyyed Amir Moradian, Sajed Khaledi, Zahra Amirkhani, Mansoureh Movahedin   +3 more
doaj   +2 more sources

In vivo and in vitro spermatogenesis in prepubertal mouse testes exposed to low gonadotoxic doses of cytarabine or Daunorubicin [PDF]

Scientific Reports
The development of experimental models treated by chemotherapy is needed for elucidating the side effects of cancer treatments administered prior to puberty on male gonad function and the feasibility of restoring fertility from exposed testicular tissues.
Marion Delessard, Laura Moutard, Coline Charnay, Nathalie Rives, Ludovic Dumont, Magali Basille-Dugay, Aurélie Feraille, Christine Rondanino   +7 more
doaj   +2 more sources

Maturation-associated changes in Sertoli cells following in vitro culture of frozen-thawed prepubertal mouse testicular tissue [PDF]

Frontiers in Reproductive Health
To preserve the fertility of prepubertal boys with cancer, testicular tissue can be cryopreserved in the hope of subsequently inducing their maturation after transplantation or in vitro culture for natural conception or medically assisted reproduction ...
Laura Moutard, Laura Moutard, Marion Dufour, Nathalie Rives, Nathalie Rives, Nathalie Rives, Magali Basille-Dugay, Magali Basille-Dugay, Eva Chemin, Eva Chemin, Valentin Rousseau, Valentin Rousseau, Aurélie Feraille, Aurélie Feraille, Aurélie Feraille, Christine Rondanino, Christine Rondanino, Ludovic Dumont, Ludovic Dumont   +18 more
doaj   +2 more sources

Understanding the Underlying Molecular Mechanisms of Meiotic Arrest during In Vitro Spermatogenesis in Rat Prepubertal Testicular Tissue [PDF]

International Journal of Molecular Sciences, 2022
Ludovic Dumont, Nathalie Rives, Marion Delessard   +2 more
exaly   +2 more sources

Artificial Gametogenesis and In Vitro Spermatogenesis: Emerging Strategies for the Treatment of Male Infertility [PDF]

International Journal of Molecular Sciences
Kaltsas A, Kyrgiafini MA, Markou E, Koumenis A, Mamuris Z, Dimitriadis F, Zachariou A, Chrisofos M, Sofikitis N.   +8 more
exaly   +2 more sources

In vitro spermatogenesis in isolated seminiferous tubules of immature mice. [PDF]

PLoS One, 2023
Mouse spermatogenesis, from spermatogonial stem cell proliferation to sperm formation, can be reproduced in vitro by culturing testis tissue masses of neonatal mice. However, it remains to be determined whether this method is also applicable when testis tissues are further divided into tiny fragments, such as segments of the seminiferous tubule (ST), a
Feng X, Matsumura T, Yamashita Y, Sato T, Hashimoto K, Odaka H, Makino Y, Okada Y, Nakamura H, Kimura H, Fujii T, Ogawa T.   +11 more
europepmc   +5 more sources

Cryopreservation of testis tissues and in vitro spermatogenesis. [PDF]

Reprod Med Biol, 2016
AbstractCancer treatments, either chemo‐ or radiotherapy, may cause severe damage to gonads which could lead to the infertility of patients. In post‐pubertal male patients, semen cryopreservation is recommended to preserve the potential to have their own biological children in the future; however, it is not applicable to prepubertals.
Yokonishi T, Ogawa T.
europepmc   +4 more sources

Discovering in vitro spermatogenesis stimulating factors. [PDF]

Cell Death Dis, 2015
Spermatozoan development encompasses pre-meiotic, meiotic and post-meiotic cellular processes adapted to genetically diversify and then vertically transmit haploid genomes via fertilization. Defined culture systems supporting spermatogonial stem cell differentiation through meiosis will provide long-sought-after experimental platforms to study ...
Chaudhary J, Hamra FK.
europepmc   +4 more sources

Models of in vitro spermatogenesis [PDF]

Spermatogenesis, 2012
Understanding the mechanisms that lead to the differentiation of male germ cells from their spermatogonial stem cells through meiosis to give rise to mature haploid spermatozoa has been a major quest for many decades. Unlike most other cell types this differentiation process is more or less completely dependent upon the cells being located within the ...
Hunter, D., Anand Ivell, R., Danner, S., Ivell, R.   +3 more
openaire   +3 more sources