Results 161 to 170 of about 1,364,664 (267)

Therapeutic profiling of dihydroartemisinin using in vitro and in vivo models reveals anti-glioma potential. [PDF]

Cancer Cell Int
Sulava S, Jalli DR, Chawla S, Senapati S, Alone DP.   +4 more
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

In Vivo Models of Diabetes: Unravelling Molecular Pathways in Metabolic and Skeletal Complications. [PDF]

Biomedicines
Ahmad Hairi H, Mustafa NH, Shuid AN, Sadikan MZ.   +3 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Comparative efficacy of chitosan and calcium carbonate-based scaffolds for bone regeneration in preclinical in vivo models: a systematic review. [PDF]

Saudi Dent J
Marampa WR, Febrida R, Djustiana N.
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Multi-Target Neuroprotective Compound Exhibits EAAT2-Modulating and Alzheimer's Pathology-Attenuating Effects in In Vitro and In Vivo Models. [PDF]

ACS Chem Neurosci
Hacımüftüoğlu A, Saraçoğlu N, Saffour S, Abad N, Kesgun Y, Zegheb N, Gundeger E, Yeşilyurt F, Ataş MN, Bati-Ayaz G, Altunlu Ö, Çınar B, Yörük MA, Okkay U, Özkaraca M, Ateş O, Taghizadehghalehjoughi A, Lafzi F, Türkez H.   +18 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Validation of chemoresistance phenotypes in pleural mesothelioma across 2D, 3D, and in vivo models. [PDF]

Sci Rep
Shi H, Selvamani SP, Zelei R, Zhuang L, Wang D, Johnson B, Dharwal V, Xu D, Wang Y, Rutland T, Klebe S, Kao S, Linton A, Hosseini-Beheshti E, Cheng YY.   +14 more
europepmc   +1 more source