Results 91 to 100 of about 115,467 (307)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Experimental plans with incomplete blocks in plant breeding

, 2016
Plant breeders can choose among different types of experimental designs. In early stages of breeding programmes a large amount of experimental genotypes need to be tested.
Zorić, Miroslav, Stojaković, Milisav, Mitrović, Bojan, Nastasić, Aleksandra, Bekavac, Goran, Stanisavljević, Dušan   +5 more
core  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

On the study of balanced incomplete block designs with repeated blocks and other incomplete block designs

, 2004
Not ...
Sharma, Rajeshkumar
core  

Balanced Incomplete Blocks to Control Individual Differences

, 1988
Balanced incomplete block designs are used when there are reasons that preclude the use of repeated measurements designs, particularly when there are at least two factors, each with several levels.
Becker, Judith A., Kimmel, Herbert D., Herbert D. Kimmel, Bevill, Michael J., Michael J. Bevill, Judith A. Becker   +5 more
core   +1 more source

Handling the Complexity of Computing Maximal Consistent Blocks

, 2023
The maximal consistent blocks technique, adopted from discrete mathematics, describes the maximal collection of objects, in which all objects are indiscernible in terms of available information.
Teresa Mroczek
core   +1 more source

Experimentos em parcelas subdivididas com tratamentos primários em blocos incompletos parcialmente balanceados I: uma solução das equações normais Split-plot experiments with main treatments in partially balanced incomplete blocks I: a solution of the normal equations

Scientia Agricola, 1995
O objetivo deste trabalho foi realizar um estudo para obtenção de uma solução adequada das equações normais de experimentos em parcelas subdivididas com tratamentos primários dispostos segundo uma estrutura de blocos incompletos parcialmente balanceados ...
A.R. de Morais, M.C Stolf Nogueira
doaj   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source