Results 101 to 110 of about 174,544 (303)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Physiological and ecological studies of mannitol utilizing marine bacteria

open access: yes, 1985
Bibliography: leaves 166-191Bacteria were isolated from the kelp beds on the West Coast of South Africa. Strains isolated from the water column and kelp fronds were classified as Pseudomonas, Vibrio, Acinetobacter and Flavobacterium species.
Davis, Claire Louise
core  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017

open access: yesEFSA Journal, 2019
The data on antimicrobial resistance in zoonotic and indicator bacteria in 2017, submitted by 28 EU Member States (MSs), were jointly analysed by EFSA and ECDC.
European Food Safety Authority   +1 more
doaj   +1 more source

Comparison of fecal indicators with pathogenic bacteria and rotavirus in groundwater

open access: yesScience of The Total Environment, 2012
Groundwater is routinely analyzed for fecal indicators but direct comparisons of fecal indicators to the presence of bacterial and viral pathogens are rare. This study was conducted in rural Bangladesh where the human population density is high, sanitation is poor, and groundwater pumped from shallow tubewells is often contaminated with fecal bacteria.
Andrew S, Ferguson   +18 more
openaire   +3 more sources

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

open access: yesFEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa   +2 more
wiley   +1 more source

DEVELOPMENT OF NEW MEDIUM TERMED ECOL FOR DETECTION OF ESCHERICHIA COLI [PDF]

open access: yesIranian Journal of Public Health, 1998
A new medium, termed Ecol was developed for the detection and enumeration of E. coli. The selective properties of Ecol medium for the detection of E.coli from mixed culture in wastewater was tested and compared with conventional coliform media at 37°C ...
M.K. Sharifi-Yazdi;C. Azimi
doaj   +2 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Bacterial Pathogen Occurrence and Persistence in Livestock Mortality Biopiles

open access: yesResources, 2017
Properly managed biopiles can be used for slaughterhouse-residual degradation and bacterial pathogen inactivation, which otherwise represent a major health risk in the environment.
Robert Michitsch   +3 more
doaj   +1 more source

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