Results 111 to 120 of about 174,544 (303)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Exploring fecal indicator bacteria in a constructed stormwater wetland

Water Science and Technology, 2011
Microbial pollution in surface waters is a concern throughout the world, with both public health and economic implications. One contributing source to such pollution is stormwater runoff, often treated using various types of stormwater control measures.
J M, Hathaway, W F, Hunt, A K, Graves, K L, Bass, A, Caldwell   +4 more
openaire   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Breeding of broad-spectrum bacteriocin-producing lactic acid bacteria in traditional fermented dairy products [PDF]

Zhongguo niangzao
To breed broad-spectrum bacteriocin-producing lactic acid bacteria, 96 strains of lactic acid bacteria isolated and purified from tradition fermented dairy products in Gansu province were screened by agar diffusion assay, and the bacteriocin-producing ...
LIU Caiyun, MA Heping, ZHANG Wenqi, WANG Jie, PENG Zhangpu, SHAO Jianning
doaj   +1 more source

Spatial and temporal heterogeneity of bacteria across an intertidal shellfish bed: Implications for regulatory monitoring of faecal indicator organisms

, 2015
Routine bacterial monitoring of shellfish beds using indicator species is a common global practice designed to prevent human consumption of contaminated shellfish products.
Wilson, James, Quilliam, Richard, Jones, David L, Clements, Katie, Malham, Shelagh K   +4 more
core   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

A novel indicator plant to test the hypersensitivity of phytopathogenic bacteria

Journal of Microbiological Methods, 2008
Hypersensitive response is an important, definitive test to separate plant pathogenic bacteria from saprophytes. The current standard protocol uses tobacco and four o'clock plants as indicators for Gram negative and Gram positive phytopathogenic bacteria, respectively, and inoculation is accomplished by infiltrating bacterial suspensions into intact ...
Umesha, S., Richardson, P. A., Kong, P., Hong, C. X.   +3 more
openaire   +3 more sources

Colony count of fecal contamination indicator bacteria per 100 ml sampled water of study area.

, 2020
Colony count of fecal contamination indicator bacteria per 100 ml sampled water of study area.
Sirak Robele Gari (8468751), Bezatu Mengistie Alemu (6894185), Negasa Eshete Soboksa (8468748), Abebe Beyene Hailu (8468754)   +3 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes

, 2013
A study was made of diurnal changes in the heterotrophic uptake kinetics of both ¹⁴C-glucose and ¹⁴C- glycolate in two small stratified lakes, Lake Rotomanuka in New Zealand and Lake Fryxell in Antarctica.
Rawley, Brian Andrew
core