Results 231 to 240 of about 2,085,056 (273)

Development of novel monoclonal antibodies for detection of pan-Lassa virus. [PDF]

PLoS Negl Trop Dis
Paroha R, Saito T, Yamada S, Click C, Paessler S, Maruyama J.   +5 more
europepmc   +1 more source

Indirect Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Cold Spring Harbor Protocols, 2017
The indirect competitive ELISA (indirect cELISA) pits plate-immobilized antigen against antigens in solution for binding to antigen-specific antibody. The antigens in solution are in the test sample and are first incubated with antigen-specific antibody.
Thomas O, Kohl, Carl A, Ascoli
openaire   +4 more sources

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Development of an indirect competitive ELISA for the detection of ABO blood group antigens

Legal Medicine, 2014
We developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the detection of ABO blood group antigens in human samples; in particular for blood stains. ABO blood group antigens conjugated to polyacrylamide were used for immobilized antigen.
Tomohiro Takayama
exaly   +3 more sources

Amperometric Immunoassay of Azinphos‐Methyl in Water and Honeybees Based on Indirect Competitive ELISA [PDF]

Analytical Letters, 2008
An electrochemical immunosensor based on indirect competitive ELISA technique has been developed and tested for the detection of azinphos-methyl in aqueous solutions and spiked honeybee extracts. The detection of the pesticide was based on competition for binding to monoclonal antibodies with an ovalbumin (OVA) conjugate, followed by the incubation ...
A. Ivanov, G. Evtugyn, H. Budnikov, GIROTTI, STEFANO, GHINI, SEVERINO, FERRI, ELIDA NORA, A. Montoya, J. V. Mercader   +7 more
core   +6 more sources

Direct and indirect competitive monoclonal antibody-based ELISA of Aflatoxin B1 in groundnut

Mycotoxin Research, 1993
Direct and indirect competitive enzyme-linked immunosorbent assays were optimized for the determination of aflatoxin B1 in groundnut utilizing a specific monoclonal antibody developed at the University of Strathclyde, UK. The monoclonal antibody was conjugated to horseradish peroxidase (HRP) for direct competitive assay, while a commercially available ...
N, Ramakrishna, V K, Mehan
exaly   +3 more sources

Development of an indirect competitive ELISA for the determination of papaverine

Talanta, 2005
Papaverine (1-(3,4-dimethoxybenzyl)-6,7-dimethoxyisoquinoline, PAP) is a member of the benzylisoquinoline sub-group of the opium alkaloids. It has been widely used for treating diseases like pulmonary arterial embolism and renal or biliary colic. In this paper, a specific conjugate of mono-demethylated papaverine-O-carboxylmethyl ether (MDMPAP-O-CME ...
Jin, Yan, Jian-Qiu, Mi, Jian-Tao, He, Zhen-Quan, Guo, Mei-Ping, Zhao, Wen-Bao, Chang   +5 more
openaire   +2 more sources

AN INDIRECT COMPETITIVE ELISA FOR DETERMINATION OF CITRININ

Journal of Food Safety, 2011
Citrinin (CIT)–protein artificial antigen was prepared by conjugating CIT with keyhole limpet hemocyanin by 1,4-butanediol diglycidyl ether. By immunization and fusion, a hybridoma cell line named K2-F3, which stably secreted the monoclonal antibody (McAb) against CIT was obtained.
YONGNING LI, YUANYUAN WANG, YANGHAO GUO
openaire   +1 more source

The preparation of polyclonal antibody against chlordimeform and establishment of detection by indirect competitive ELISA

Journal of Environmental Science and Health, Part B, 2022
Objective: Chlordimeform is a chemical pesticide that is highly carcinogenic and toxic. The purpose of this study was to establish an enzyme-linked immunosorbent assay (ELISA) method for the detection of chlordimeform in aquaculture and fish farming.Chlordimeform was coupled with bovine serum albumin (BSA) and ovalbumin (OVA) as carrier proteins.
Yang-Mei-Qing, Hu, Hua-Wei, Feng, Shuai, Liu, Chang, Liu, Peng-Yan, Zhao, Man, Zhang, Li, Zhang, Jian, Zhao, Jia-Zeng, Li, Xiao-Miao, Yu, Hong-Sheng, Liu, Jun-Feng, Zhu   +11 more
openaire   +2 more sources

Determination of Multiresidue of Avermectins in Bovine Liver by an Indirect Competitive ELISA

Journal of Agricultural and Food Chemistry, 2006
An ELISA was developed to detect multiresidues of avermectins (AVMs) including abamectin (ABM), ivermectin (IVM), and eprinomectin (EPR) in bovine liver. The modified ABM, 4'-O-succinoyl-ABM was conjugated to bovine serum albumin as the immunogen for the preparation of polyclonal antibodies to AVMs and conjugated to ovalbumin as the coating antigen for
Weimin, Shi, Jihong, He, Haiyang, Jiang, Xiaolin, Hou, Junhong, Yang, Jianzhong, Shen   +5 more
openaire   +2 more sources

Deoxynivalenol in commercial beer — screening for the toxin with an indirect competitive ELISA

Mycotoxin Research, 1993
Antibodies specific for deoxynivalenol (DON) were prepared by immunizing rabbits with a deoxynivalenol-hemiglutaryl-HSA conjugate. The antibody showed cross-reactivity with DON, 15a-acetyl-DON (15-Ac DON), and 3a-acotyl-DON (3-Ac DON) of 100%, 216%, and 260%, respectively. No cross-reactivity was observed against 20 further trichothecenes of the A-, B-,
L, Niessen, M, Böhm-Schrami, H, Vogel, S, Donhauser   +3 more
openaire   +2 more sources