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Preparation of a Polyclonal Antibody Based Indirect Competitive ELISA for Enrofloxacin Residue
Advanced Materials Research, 2012A rapid indirect competitive ELISA (icELISA) for the determination of enrofloxacin (ENR) residue has been developed. EDC method was employed to synthesize the artificial antigen of ENR-BSA, and anti-serum produced from rabbits was selected. Based on the square matrix titration, an icELISA method was developed with the polyclonal antibody.
Guo Ying Fan, Jin Qing Jiang
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Development of an Indirect Competitive ELISA for Analysis of Alternariol in Bread and Bran Samples
Food Analytical Methods, 2017Alternariol (AOH) is one of the major mycotoxins produced by various species of Alternaria fungi. Natural occurrences of AOH have been reported in various foods, including fruits; processed fruit products such as apple juice, tomato products; wheat and other grains; oilseeds and products thereof, such as sunflower seeds, oilseed rape meal, and flax ...
Gurmit Singh +5 more
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Use of the indirect competitive ELISA for the detection of Brazil nut in food products
Food Control, 2004Food related allergic reactions following inadvertent ingestion are increasingly common, with nuts, including Brazil nut, placed firmly in the top 10 food groups whose presence within a product should be declared. The presence of hidden allergens as a result of adulteration or contamination of ingredients presents a problem for both the food industry ...
Alfonso Clemente +4 more
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[Detection of microcystin-LR in waters using indirect competitive ELISA].
Huan jing ke xue= Huanjing kexue, 2008Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established to detect microcystin-LR in waters, with the concentration of the complete antigen was 5microg/mL, the dilution of the monoclonal antibody was 1:3,000, the dilution of the enzyme tracer (goat anti-rabbit IgG-peroxidase) was 1:3,000, the concentration range of microcystin ...
Jian-wu, Sheng +4 more
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Development of an Indirect Competitive ELISA Kit for the Rapid Detection of Benzopyrene Residues
Food Analytical Methods, 2015This paper presents the generation of monoclonal antibodies (mAbs) with high specificity against benzopyrene (BaP) through cell fusion procedures, and the development of mAb-based indirect competitive enzyme-linked immunoabsorbent kit (icELISA kit) to detect BaP using one of these hybridomas (clone 2E12).
Jun Xi, QiaoQiao Shi, QiYu Lu
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[A indirect competitive ELISA to detect domoic acid in seawater and shellfish].
Wei sheng yan jiu = Journal of hygiene research, 2012A competitive enzyme-linked immunosorbent assay (ELISA) was developed for measurent domoic acid (DA) in seawater and shellfish.DA was coupled to bovine serum albumin (BSA) and ovalbumin (OVA) by carbodiimide reaction. DA-BSA as immunogen was injected in BALB/c mice.
Renyan, Liu +3 more
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Determination of Cd-MT in Pteria penguin with indirect non-competitive ELISA
Wuhan University Journal of Natural Sciences, 2011Cadmium-metallothionein (Cd-MT) of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme-linked immunosorbent assay (non-competitive ELISA) was established to detect shellfish Cd-MT.
Xiaoping Wu +5 more
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