Results 111 to 120 of about 7,556,090 (298)

Microfabricated Devices for Adherent Stem Cell Culture [PDF]

, 2015
This thesis details the development of a system of microfabricated devices for the adherent culture of stem cells. The multipotency and self renewal of stem cells make them a potentially abundant source of valuable human cells, for both drug screening ...
Macown, RJ
core  

NeuroSuite for Long‐Term Functional and Structural Studies of Air‐Liquid Interface Cerebral Organoids

Advanced Science, EarlyView.
NeuroSuite provides a modular hardware‐software platform integrating Neuroweb and NeuroMaps to enable long‐term, in situ electrophysiological interrogation of air–liquid interface organoid slices while preserving tissue architecture. Its components can be used together or independently to capture real‐time activity, spatial network dynamics, and ...
Belquis Haider, Sagnik Middya, Daniel J. Lloyd‐Davies‐Sánchez, Nino F. Läubli, Sulay Vora, Jakob Träuble, Rohan N. Krajeski, Yuqing Feng, Daniel Duncko, Josiah S. Riley, Rubén Ruiz‐Mateos Serrano, Ole Paulsen, Madeline A. Lancaster, George G. Malliaras, Gabriele S. Kaminski Schierle   +14 more
wiley   +1 more source

Innate Immunocompetent hiPSC‐Derived Neurospheroids Capture Early CNS Responses to rAAV

Advanced Science, EarlyView.
Knowledge of human CNS immune responses to AAV‐based gene therapies remains limited due to the lack of immune‐competent human models. Here, a hiPSC‐derived 3D neuroimmune platform integrating neurospheroids and microglia is established using stirred‐tank bioreactors.
Catarina M. Gomes, Gabriela Silva, Mafalda Aleixo, Marta Gomes, Daniel Simão, Stephan J. Holtkamp, Diana D. Lobo, Pradeep Harish, Rosalind Jenkins, Lekh N. Dahal, Rui J. Nobre, Luís de Pereira de Almeida, Mark Trautwein, Paula M. Alves, Catarina Brito   +14 more
wiley   +1 more source

Efficient Production of Hepatocyte-like Cells from Human-induced Pluripotent Stem Cells by Optimizing Growth Factors

International Journal of Organ Transplantation Medicine, 2018
Background: Generating hepatocytes with complete liver functions is still a challenge and developing more functional hepatocytes is needed. Objective: To compare various differentiation factors and protocols and introducing a preferable protocol to ...
Z Jaafarpour, M Soleimani, S Hosseinkhani, M H Karimi, P Yaghmaei, N Mobarra, B Geramizadeh   +6 more
doaj  

Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos

Stem Cells International, 2014
Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected ...
Jitong Guo, Baojiang Wu, Shuyu Li, Siqin Bao, Lixia Zhao, Shuxiang Hu, Wei Sun, Jie Su, Yanfeng Dai, Xihe Li   +9 more
doaj   +1 more source

Charting Endocrine Progenitors Across Species and Organs

Advanced Science, EarlyView.
Endocrine progenitors give rise to the hormone‐producing cells of the pancreas and intestine. Using single‐cell multiomics and proteomics, this study compares these progenitors across species, systems, and organs, mapping the conserved and species‐specific gene regulatory networks that guide their formation.
Changying Jing, Michael Sterr, Özüm Sehnaz Caliskan, Lama Saber, Nicole Katarina Rogers, Samuel Ogden, Kei Kozawa, Christos Karampelias, Jessica Jaki, Aimée Bastidas‐Ponce, Falk Janos Farkas, Oliver Czarnecki, Osvaldo Rodrigues Pereira, Perla Cota, Valerie Vandenbempt, Diego Balboa, Anika Böttcher, Natalie Krahmer, Mohammad Lotfollahi, Julie B. Sneddon, Heiko Lickert, Mostafa Bakhti   +21 more
wiley   +1 more source

Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells [PDF]

, 2012
Human mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically.
Vanleene, Maximilien, George Bou-Gharios (139817), Moschidou Dafni, Gemma N Jones, Maximilien Vanleene (139811), George Bou-Gharios, Puga-Iglesias, Tamara-Isabel, Katarzyna Kuleszewicz, Sandra J. Shefelbine (139814), David, AL, Shefelbine, Sandra J., Fisk, NM, David Anna L., Moschidou, Dafni, Shefelbine Sandra J., Guillot, Pascale V., Bou-Gharios George, Gemma N. Jones (139797), Maximilien Vanleene, Fisk Nicholas M., Nicholas M Fisk, Shefelbine, SJ, De Coppi, Paolo, Puga-Iglesias, TI, Puga-Iglesias Tamara-Isabel, Katarzyna Kuleszewicz (139808), Paolo De Coppi (118582), Jones Gemma N., Bou-Gharios, G, Pascale V. Guillot (139827), Kuleszewicz Katarzyna, Anna L David, Vanleene, M, Kuleszewicz, K, David, Anna L., Sandra J Shefelbine, Fisk, Nicholas M., Dafni Moschidou, Paolo De Coppi, Bou-Gharios, George, Tamara-Isabel Puga-Iglesias, Pascale V Guillot, Jones, GN, Tamara-Isabel Puga-Iglesias (139805), Guillot, PV, De Coppi Paolo, Fisk, NM ; https://orcid.org/, Jones, Gemma N., Dafni Moschidou (139801), Guillot Pascale V., Kuleszewicz, Katarzyna, Moschidou, D, Vanleene Maximilien, Nicholas M. Fisk (139820), De Coppi, P, Anna L. David (139823)   +55 more
core   +1 more source

Disrupted TFEB/GDNF–cAMP/ATP Coupling Underlies Astrocytic Dysfunction and Depression in LRRK2 G2019S Parkinson's Mice

Advanced Science, EarlyView.
ABSTRACT The LRRK2 G2019S mutation, a Parkinson's disease–linked variant, has been associated with depression‐like phenotypes, but mechanisms remain unclear. We chart age‐dependent behavioral changes and astrocyte reactivity in G2019S mice and define a pathway connecting LRRK2 to TFEB/GDNF signaling, cellular energetics, and inflammation.
Longping Yao, Maryam Hatami, Sumeyye Koc, Qing Jiang, Thomas Skutella, Yuanfeng Zhang   +5 more
wiley   +1 more source

Co‐Delivery of Sustained Release Chondroitinase ABC‐37 With Human iPSC‐Derived Neural Progenitors Promotes Transplant Survival and Functional Recovery in a Rodent Model of Stroke

Advanced Science, EarlyView.
A regenerative strategy for stroke combines human stem cell–derived neural progenitor cells with sustained release of a matrix‐modifying, thermostable chondroitinase ABC‐37 enzyme. In a rat model of stroke, co‐delivery enhanced transplanted cell survival and neuronal differentiation, degraded inhibitory extracellular matrix components, and improved ...
Nitzan Letko Khait, David Xinzheyang Li, Keyi Guo, Hong Cui, Quinton Sirianni, Dhana Abdo, Cindi M. Morshead, Molly S. Shoichet   +7 more
wiley   +1 more source

Engineering Approaches to Modify Immunomodulatory Functions of Mesenchymal Stromal Cells (MSCs): Tissue Regeneration and Clinical Application

Advanced Science, EarlyView.
Mesenchymal stromal cells (MSCs) show promise for treating immune‐related disorders through immunomodulation and tissue regeneration. This review gives a brief overview of current clinical approval of MSC therapies. It also discussed how bioengineering, including genetic modification, biomaterial delivery, extracellular vesicles, and iPSC‐derived MSCs,
Sichen Yang, Kejia Li, Ning Wang, Zhen Li, Zhiyong Zhang, Rocky S. Tuan, Yangzi Jiang   +6 more
wiley   +1 more source