Results 111 to 120 of about 225,831 (315)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

INFERTILITY AROUND THE GLOBE: NEW THINKING ON CHILDLESSNESS, GENDER, AND REPRODUCTIVE TECHNOLOGIES [PDF]

, 2002
Pt. I. Discourses and debates -- Ch. 1. Introduction. Interpreting infertility: a view from the social sciences / Frank van Balen and Marcia C. Inhorn -- Ch. 2.

core  

Infertility, Assisted Reproductive Technologies and Hormone Assays [PDF]

, 2019
Infertility is a major public health concern and medical condition that afflicts millions globally. As such, many people seek reproductive care with the goal of receiving a proper diagnosis and a successful outcome using assisted reproductive technology (

core   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Distribution of infertility factors among infertile couples in Yemen [PDF]

, 2016
Background: Infertility is a global health issue and determines as a one of the most prevalent health disorders among young adult couples. It is well known that 50-80 million people are facing the problem of getting an integrated family.
Kaid, Belqees, Allow, Ahmed Kaid Naji, Bracamonte, Maryam, Abdulmogny A, Sadek S. M.   +3 more
core   +1 more source

Intracytoplasmic sperm injection versus conventional in vitro insemination in couples with non-male infertility factor in the ‘real-world’ setting: analysis of the HFEA registry

Journal of Translational Medicine
Background In assisted reproductive technology (ART), the choice between intracytoplasmic sperm injection (ICSI) and conventional in vitro insemination (IVF) remains a pivotal decision for couples with female or unexplained infertility.
Alessio Paffoni, Amerigo Vitagliano, Laura Corti, Edgardo Somigliana, Paola Viganò   +4 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

The Palgrave Handbook of Infertility in History [PDF]

, 2017
This ground-breaking, interdisciplinary volume provides an overdue assessment of how infertility has been understood, treated and experienced in different times and places.

core   +1 more source

Physician Management of Non-Cavity Distorting Intramural Fibroids and Infertility: A Survey of REI Providers

International Journal of Women's Health
Megan K Wasson,1 Daniela Diego,1,2 John C Petrozza,3,4 Jennifer F Kawwass,1,2 Michael F Neblett 2nd5 1School of Medicine, Emory University, Atlanta, GA, USA; 2Department of Gynecology & Obstetrics, Division of Reproductive Endocrinology & ...
Wasson MK, Diego D, Petrozza JC, Kawwass JF, Neblett MF 2nd   +4 more
doaj  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source