Results 201 to 210 of about 32,598 (225)

Simultaneous detection of influenza virus type B and influenza A virus subtypes H1N1, H3N2, and H5N1 using multiplex real-time RT-PCR

Applied Microbiology and Biotechnology, 2011
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza type B virus and influenza A virus subtypes H5N1, H3N2, and H1N1 has been described. The method exhibited a high specificity and sensitivity of approximately 10(1)-10(2) copies per microliter or 10(-3)-10(-2) TCID50/L for each
Fang, Shisong, Li, Jianxiong, Cheng, Xiaowen, Zhao, Cunyou, Wang, Ting, Lv, Xing, Wang, Xin, Wu, Chunli, Zhang, Renli, Cheng, Jinquan, Hong, Xue, Yu, Muhua   +11 more
openaire   +3 more sources

Multiplex RT-PCR assay for differentiating European swine influenza virus subtypes H1N1, H1N2 and H3N2

Journal of Virological Methods, 2012
In Europe, three major swine influenza viral (SIV) subtypes (H1N1, H1N2 and H3N2) have been isolated in pigs. Developing a test that is able to detect and identify the subtype of the circulating strain rapidly during an outbreak of respiratory disease in the pig population is of essential importance.
Chiara, Chiapponi, Ana, Moreno, Ilaria, Barbieri, Marianna, Merenda, Emanuela, Foni   +4 more
openaire   +2 more sources

Protection against a European H1N2 swine influenza virus in pigs previously infected with H1N1 and/or H3N2 subtypes

Vaccine, 2003
A novel swine influenza virus, H1N2, circulates in European swine populations together with H1N1 and H3N2 viruses. This study examines whether post-infection immunity to H1N1 and/or H3N2 viruses provides cross-protection against H1N2 infection. Pigs (n=51) were inoculated intranasally with either Sw/Belgium/1/98 (H1N1) or Sw/Flanders/1/98 (H3N2), or ...
Kristien, Van Reeth, Vicki, Gregory, Alan, Hay, Maurice, Pensaert   +3 more
openaire   +2 more sources

Amplification of the entire genome of influenza A virus H1N1 and H3N2 subtypes by reverse-transcription polymerase chain reaction

Journal of Virological Methods, 2006
This study describes the development of a simple RT-PCR method to amplify the whole genome of the influenza A virus based on the amplification of full-length gene segments. Primers were designed based on the conserved regions of both the 5'-end and the 3'-end of each gene segment.
Chi-Ho, Chan, Keh-Liang, Lin, You, Chan, Ya-Li, Wang, Yu-Tsai, Chi, Hsiao-Li, Tu, Happy-K, Shieh, Wu-Tse, Liu   +7 more
openaire   +2 more sources

[Evolution of the hemagglutinin gene of influenza A virus H1N1-subtype (1977-1983].

Molekuliarnaia genetika, mikrobiologiia i virusologiia, 1987
A scheme for evolutionary interrelations of the H1-subunits of influenza hemagglutinin genes is proposed for the natural variants of influenza A virus of the H1N1-subtype. It is based on experimental data obtained by the authors and those reported in the literature.
S V, Netesov, N A, Petrov, V M, Blinov, V A, Karginov   +3 more
openaire   +1 more source

Digest: Reassortment-based evolution of H1N1 subtype swine influenza virus in China

Evolution
Abstract In a new study, Zhao et al. (2025) obtain 959 whole genome sequences of H1N1 subtype swine influenza virus (SIV) isolated from China. Their analysis of the sequences, isolated between 1977 and 2020, reveals how H1N1 lineages have co-evolved and contributed to instances of zoonotic transmission within the region.
openaire   +2 more sources

Rapid identification of neuraminidase inhibitor resistance mutations in seasonal influenza virus A(H1N1), A(H1N1)2009, and A(H3N2) subtypes by melting point analysis

European Journal of Clinical Microbiology & Infectious Diseases, 2011
The high mutation rate of influenza virus, combined with the increasing worldwide use of influenza virus-specific drugs, allows the selection of viruses that are resistant to the currently available antiviral medications. Therefore, reliable tests for the rapid detection of drug-resistant influenza virus strains are required.
M, Redlberger-Fritz, S W, Aberle, R, Strassl, T, Popow-Kraupp   +3 more
openaire   +2 more sources

[Isolation, identification and genetic analysis of an H1N1 subtype isolate of swine influenza virus].

Bing du xue bao = Chinese journal of virology, 2010
In 2006, a swine influenza virus (SIV) isolate was isolated from 30 nasal swabs samples collected from pigs with clinical syndromes of swine influenza in a pig farm of Liaoning Province. The virus isolate was studied and identified by the growth in 9-11 days old chicken embryo, hemagglutination (HA) assay, hemagglutination inhibition (HI) assay ...
Wei, Lu, Xiu-hua, Zhang, Xiu-dong, Wang, Hua, Wu   +3 more
openaire   +1 more source

Comparison of neuraminidase activity of influenza A virus subtype H5N1 and H1N1 using reverse genetics virus.

The Southeast Asian journal of tropical medicine and public health, 2010
Neuraminidase (NA) is an envelope surface glycoprotein of influenza A viruses. It cleaves alpha-(2,3) or alpha-(2,6) glycosidic linkage between a terminal sialic acid residue of the host cell receptor and hemagglutinin of the viral envelope, thus releasing viral progeny from the infected cell.
Anchalee, Rawangkhan, Donruedee, Sanguansermsri, Narutchala, Suwannakhon, Sutatip, Pongcharoen, Purinthra, Pensuwan, Chanpen, Chamnanpood, Pornchai, Chamnanpood, Phanchana, Sanguansermsri   +7 more
openaire   +1 more source

Comparison of Hemagglutinin and Neruaminidase of Influenza A Virus Subtype H1N1, H5N1, H5N2, and H7N9 Using Apriori Algorithm

2014
The Spanish flu first occurred in 1918 and killed about 50 million people in the world. In 2005, by using gene decoding process, Robert B. Belshe identified that the Spanish flu was occurred by H1N1, which is highly pathogenic influenza A virus. Influenza A virus has been mutated consistently and unexpectedly; H5N1, H5N2, and H7N9 which used to be ...
Dae Young Kim, Hye-Jun Kim, Junhyeok Bae, Taeseon Yoon   +3 more
openaire   +1 more source