Results 211 to 220 of about 48,268 (246)
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Archives of Virology, 2014
Swine influenza (SI) is an acute, highly contagious respiratory disease caused by swine influenza A viruses (SwIVs), and it poses a potential global threat to human health. Classical H1N1 (cH1N1) SwIVs are still circulating and remain the predominant subtype in the swine population in China.
Fu-Ru Yang +7 more
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Swine influenza (SI) is an acute, highly contagious respiratory disease caused by swine influenza A viruses (SwIVs), and it poses a potential global threat to human health. Classical H1N1 (cH1N1) SwIVs are still circulating and remain the predominant subtype in the swine population in China.
Fu-Ru Yang +7 more
openaire +3 more sources
Journal of Interferon & Cytokine Research, 2011
Interferons (IFNs) are critically important in the control of influenza A virus infections. To better understand the pathogenic characteristics of the pandemic 2009 H1N1 influenza virus (pH1N1) from an innate immunity viewpoint, we investigated whether in vitro pH1N1 is sensitive to the antiviral activity of IFN beta, leukocyte IFN alpha, and several ...
SCAGNOLARI, CAROLINA +9 more
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Interferons (IFNs) are critically important in the control of influenza A virus infections. To better understand the pathogenic characteristics of the pandemic 2009 H1N1 influenza virus (pH1N1) from an innate immunity viewpoint, we investigated whether in vitro pH1N1 is sensitive to the antiviral activity of IFN beta, leukocyte IFN alpha, and several ...
SCAGNOLARI, CAROLINA +9 more
openaire +3 more sources
Journal of Virological Methods, 2012
In Europe, three major swine influenza viral (SIV) subtypes (H1N1, H1N2 and H3N2) have been isolated in pigs. Developing a test that is able to detect and identify the subtype of the circulating strain rapidly during an outbreak of respiratory disease in the pig population is of essential importance.
Emanuela Foni +4 more
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In Europe, three major swine influenza viral (SIV) subtypes (H1N1, H1N2 and H3N2) have been isolated in pigs. Developing a test that is able to detect and identify the subtype of the circulating strain rapidly during an outbreak of respiratory disease in the pig population is of essential importance.
Emanuela Foni +4 more
openaire +3 more sources
Vaccine, 2003
A novel swine influenza virus, H1N2, circulates in European swine populations together with H1N1 and H3N2 viruses. This study examines whether post-infection immunity to H1N1 and/or H3N2 viruses provides cross-protection against H1N2 infection. Pigs (n=51) were inoculated intranasally with either Sw/Belgium/1/98 (H1N1) or Sw/Flanders/1/98 (H3N2), or ...
Kristien Van Reeth +3 more
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A novel swine influenza virus, H1N2, circulates in European swine populations together with H1N1 and H3N2 viruses. This study examines whether post-infection immunity to H1N1 and/or H3N2 viruses provides cross-protection against H1N2 infection. Pigs (n=51) were inoculated intranasally with either Sw/Belgium/1/98 (H1N1) or Sw/Flanders/1/98 (H3N2), or ...
Kristien Van Reeth +3 more
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Journal of Virological Methods, 2006
This study describes the development of a simple RT-PCR method to amplify the whole genome of the influenza A virus based on the amplification of full-length gene segments. Primers were designed based on the conserved regions of both the 5'-end and the 3'-end of each gene segment.
Keh-Liang Lin +7 more
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This study describes the development of a simple RT-PCR method to amplify the whole genome of the influenza A virus based on the amplification of full-length gene segments. Primers were designed based on the conserved regions of both the 5'-end and the 3'-end of each gene segment.
Keh-Liang Lin +7 more
openaire +3 more sources
Applied Microbiology and Biotechnology, 2011
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza type B virus and influenza A virus subtypes H5N1, H3N2, and H1N1 has been described. The method exhibited a high specificity and sensitivity of approximately 10(1)-10(2) copies per microliter or 10(-3)-10(-2) TCID50/L for each
Fang, Shisong +11 more
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Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza type B virus and influenza A virus subtypes H5N1, H3N2, and H1N1 has been described. The method exhibited a high specificity and sensitivity of approximately 10(1)-10(2) copies per microliter or 10(-3)-10(-2) TCID50/L for each
Fang, Shisong +11 more
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Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2016
Surface enhanced Raman spectroscopy technology (SERS), using gold nanoparticles as a base, was developed for rapid and sensitive detection of virus strains. SERS can be used as a rapid and reliable method to distinguish the titers of viral replication.
Xiaoxiao Jia +6 more
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Surface enhanced Raman spectroscopy technology (SERS), using gold nanoparticles as a base, was developed for rapid and sensitive detection of virus strains. SERS can be used as a rapid and reliable method to distinguish the titers of viral replication.
Xiaoxiao Jia +6 more
openaire +3 more sources
European Journal of Clinical Microbiology & Infectious Diseases, 2011
The high mutation rate of influenza virus, combined with the increasing worldwide use of influenza virus-specific drugs, allows the selection of viruses that are resistant to the currently available antiviral medications. Therefore, reliable tests for the rapid detection of drug-resistant influenza virus strains are required.
Robert Strassl +3 more
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The high mutation rate of influenza virus, combined with the increasing worldwide use of influenza virus-specific drugs, allows the selection of viruses that are resistant to the currently available antiviral medications. Therefore, reliable tests for the rapid detection of drug-resistant influenza virus strains are required.
Robert Strassl +3 more
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[The rescue of H1N1 subtype swine influenza virus].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2009The swine influenza virus (SIV) strain A/Swine/TianJin/01/2004(H1N1) (A/S/TJ/04) was rescued successfully by an eight-plasmid rescue system. The cDNAs of SIV 8 gene segments were synthesized by RT-PCR and cloned into the RNA polymerase I/II bidirection expression vector PHW2000 independently, resulting in 8 recombinant plasmids.
Yaping, Peng +3 more
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2014
The Spanish flu first occurred in 1918 and killed about 50 million people in the world. In 2005, by using gene decoding process, Robert B. Belshe identified that the Spanish flu was occurred by H1N1, which is highly pathogenic influenza A virus. Influenza A virus has been mutated consistently and unexpectedly; H5N1, H5N2, and H7N9 which used to be ...
Dae Young Kim +3 more
openaire +2 more sources
The Spanish flu first occurred in 1918 and killed about 50 million people in the world. In 2005, by using gene decoding process, Robert B. Belshe identified that the Spanish flu was occurred by H1N1, which is highly pathogenic influenza A virus. Influenza A virus has been mutated consistently and unexpectedly; H5N1, H5N2, and H7N9 which used to be ...
Dae Young Kim +3 more
openaire +2 more sources