Results 171 to 180 of about 114,195,449 (202)

Route of Inoculation Determines Symptom Profile and Replication Dynamics After low Pathogenic Avian Influenza A(H7N9) Virus Infection in Ferrets. [PDF]

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Wong SS   +9 more
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Influenza A/B virus detection and influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR

Journal of Virological Methods, 2014
Infections of the novel avian influenza A H7N9 virus cause severe respiratory diseases and death. In this study, to develop highly sensitive methods for differentially detecting the H7N9 virus, multiplex and singular real-time reverse transcription polymerase chain reaction (RT-PCR) assays were established and examined by targeting the H7 and N9 genes ...
Rei-Lin, Kuo   +7 more
openaire   +2 more sources

Characterization of antibody response to an epitope spanning the haemagglutinin cleavage site of H7N9 subtype avian influenza virus for the differentiation of infected and vaccinated chickens

Avian Pathology, 2022
H7N9 subtype avian influenza virus (AIV) is endemic in poultry in China, and vaccination is used as the primary strategy for disease control. However, by current serological tests, monitoring H7N9 virus infection in vaccinated poultry is difficult because vaccine-induced antibodies are not readily distinguishable from field viruses.
Zenglei Hu   +4 more
openaire   +2 more sources

Development of a monoclonal antibody‐based antigen capture enzyme‐linked immunosorbent assay for detection of H7N9 subtype avian influenza virus

Journal of Medical Virology, 2020
AbstractTo establish a rapid detection method for H7N9 avian influenza virus (AIV), monoclonal antibodies (mAbs) against hemagglutinin (HA) of H7N9 were developed to establish an antigen‐capture enzyme‐linked immunosorbent assay (AC‐ELISA). AC‐ELISA achieved high specificity and sensitivity, with a detection limit of 3.9 ng/mL for H7N9 HA protein (A ...
Fan Yang   +5 more
openaire   +2 more sources

Rapid hemagglutinin subtyping of novel avian-origin influenza A(H7N9) virus using a diagnostic microarray

BioChip Journal, 2014
An epidemic of human H7N9 influenza virus infection has recently emerged in China, caused by the novel reassortant avian-origin influenza A virus subtype H7N9. The ability to readily identify novel strains will allow a more rapid response, in order to reduce the spread of the disease and minimize the chance of a worldwide flu pandemic.
Dong-Hun Lee   +10 more
openaire   +1 more source

OBTAINING MRNA ENCODING HEMAGGLUTININ OF INFLUENZA A VIRUS SUBTYPE H7N9 BY IN VITRO TRANSCRIPTION AND EVALUATION OF THE EFFICIENCY OF TRANSLATION OF THE OBTAINED MRNA IN THE EUKARYOTIC CELL LINE A549

X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023: Cб. тез., 2023
RNA-based vaccines are the newest promising alternative to traditional approaches to treat viral diseases. In this study, we obtained mRNA encoding the hemagglutinin of influenza A subtype H7N9 by in vitro transcription and evaluated the efficiency of its translation on the A549 cell line.
O.A. Dobrovolskaya   +2 more
openaire   +1 more source

Detection of PB2 627 K mutation in two highly pathogenic isolates of the H7N9 subtype Influenza a virus from chickens in Northern China

Journal of Infection, 2020
Abstract PB2 E627K mutation of influenza A virus has been considered relevant to increased mammalian virulence and adaptation. In H7N9, the substitution is naturally confined to most human isolates and few low pathogenic avian isolates. However, we isolated two highly pathogenic H7N9 variants harboring PB2 627K from chickens in northern China.
Jinyuan, Gu   +6 more
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[Characterization of a monoclonal antibody against the hemagglutinin stem of H7N9 subtype avian influenza virus].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2022
The conserved hemagglutinin (HA) stem region of avian influenza virus (AIV) is an important target for designing broad-spectrum vaccines, therapeutic antibodies and diagnostic reagents. Previously, we obtained a monoclonal antibody (mAb) (5D3-1B5) which was reactive with the HA stem epitope (aa 428-452) of H7N9 subtype AIV.
Jiangyan, Zhao   +4 more
openaire   +1 more source

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