Results 91 to 100 of about 724,211 (317)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Fluoroscopic lumbar interlaminar epidural injections in managing chronic lumbar axial or discogenic pain

, 2012
Laxmaiah Manchikanti,1,2 Kimberly A Cash,1 Carla D McManus,1 Vidyasagar Pampati,1 Ramsin Benyamin3,41Pain Management Center of Paducah, Paducah, KY; 2University of Louisville, Louisville, KY; 3Millennium Pain Center, Bloomington, IL; 4University
McManus CD, Cash KA, Benyamin R, Manchikanti L, Pampati V   +4 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Poly-l-lactic acid for HIV-1 facial lipoatrophy: 48-week follow-up

, 2009
OBJECTIVES: Poly-l-lactic acid (PLA) injections modestly increase objectively assessed facial thickness but not facial soft tissue volume (FSTV) over 24 weeks. The durability of this response has not been well defined objectively.
Cooper, D.A., Petoumenos, K, Watson, J., Cooper, D., Carr, A, Baker, D., Carey, D, GD Rogers, Cooper, D. A., Petoumenos, K., Chuah, John, DA Cooper, Chuah, J, Carey, D., Rogers, GD, J Chuah, S Emery, Rogers, G.D., D Carey, K Petoumenos, D Baker, A Carr, Carr, A., Nolan, D., Baker, D, Emery, S, Rogers, Gary, Rogers, G. D., Watson, J, Emery, S., Cooper, DA, Chuah, J., J Watson   +32 more
core   +1 more source

Key data security issues in mobile apps

Информатика. Экономика. Управление
The article presents a comprehensive analysis of the key challenges in ensuring information security of mobile applications amid digital transformation in the context of Industry 4.0 and 5.0 paradigms.
А. А. Грейс, Д. Р. Идрисова, Д. М. Скрябин   +2 more
doaj   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Case of endophthalmitis following intravitreal injections of bevacizumab

, 2010
Shinya Komori1, Akira Sawada1, Tsutomu Oguni1, Kiyofumi Mochizuki1, Kiyofumi Ohkusu21Department of Ophthalmology, 2Department of Microbiology, Gifu University Graduate School of Medicine, Gifu, JapanPurpose: We report a case of infectious endophthalmitis
Akira Sawada, Tsutomu Oguni, Shinya Komori, et al   +3 more
core  

How are storm time injections different from nonstorm time injections?

, 2004
One of the key elements of storms and substorms is the injection of energetic particles into the region of near geosynchronous orbit, that is, the sudden flux enhancement in the energy range of tens to hundreds of KeV.
Nam, UW, Han, WY, Lee, DY, Min, KyoungWook, Lee, ES, Hwang, JA   +5 more
core   +1 more source

Bilateral Sterile Intraocular Inflammation Following Intravitreal Aflibercept 8 mg Injections: A Case Report

Reports
Background and Clinical Significance: To report a case of bilateral sterile intraocular inflammation following intravitreal aflibercept 8 mg (Eylea HD) injections.
Ram Cohen, Tomer Kerman, Omer Trivizki
doaj   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source